CDIPT (NM_006319) Human Untagged Clone

CAT#: SC320184

CDIPT (untagged)-Human CDP-diacylglycerol--inositol 3-phosphatidyltransferase (CDIPT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDIPT
• Synonyms: PIS; PIS1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006319.3 CGGAGCGCCTGCGGGGCTGGCAGAGGCGAGGGAGGTTGCGGGTAGGAAGGGCGGACTGCG
  CGCGCCCCCTGCGTCCCGCGCACCTCGGGGCCGGTCCATGCTCCCGACGGCTGCGGGCTT
  CAGCATCTGGGGCCAGGTTGGGGCGGCGGGGTCCAGGGCGCAGGTGGTGCGGCCGATGCG
  CCGGGCCGGAGGCTGAGGCCGCGCTGCGGGGCTGGGACAGCACTGGCATCTCCAGAGCAG
  GCCCGGGGCAGCAAGGGAGGCGCCGCGATGCCAGACGAAAATATCTTCCTGTTCGTGCCC
  AACCTCATCGGTTATGCCCGGATTGTCTTCGCCATCATTTCTTTCTACTTCATGCCCTGC
  TGCCCCCTCACGGCCTCCTCCTTCTACCTGCTCAGCGGCCTGCTGGACGCTTTCGATGGA
  CACGCTGCTCGCGCTCTTAATCAAGGAACCCGGTTTGGGGCCATGCTGGACATGCTGACG
  GACCGCTGCTCCACCATGTGCCTGTTGGTCAACCTGGCCCTGCTGTACCCTGGAGCCACG
  CTGTTCTTCCAAATCAGCATGAGTTTGGATGTGGCCAGTCACTGGCTGCACCTCCACAGT
  TCTGTGGTCCGAGGCAGTGAGAGTCACAAGATGATCGACTTGTCCGGGAATCCGGTGCTT
  CGGATCTACTACACCTCGAGGCCTGCTCTGTTCACCTTGTGTGCTGGGAATGAGCTCTTC
  TACTGCCTCCTCTACCTGTTCCATTTCTCTGAGGGACCTTTAGTTGGCTCTGTGGGACTG
  TTCCGGATGGGCCTCTGGGTCACTGCCCCCATCGCCTTGCTGAAGTCGCTCATCAGCGTC
  ATCCACCTGATCACGGCCGCCCGCAACATGGCTGCCCTGGACGCAGCAGACCGCGCCAAG
  AAGAAGTGACGCTGGAGCCCCGGGTCCTGGCTGCCCACCTGCCCTGGGAGTCTTGCTGTG
  CCACACAGCTCCCCACCCCCTGCTAGGAGGTCCCAGTCTCACGCCTTCCTCATGTGTTGT
  TCTACCTGCTGGGATGGGGGTCAGCCTCTCTTTGGTGACGTCACGTTCTCTGGGATCCTG
  AGGACCCGGGCCTCAAATCAGGGAGGATACGCGGGAGGCCCCCTCCATCCAGGCGGTGCT
  CCTGGGGTGCCGGGACCGGGCAGTGTCACACCCTGCCTGCTCAGTCCTGGGGTCCGAGAT
  GCTAGGGACGCTTGAGTGAGGGAGGTGGTGTGAGGGCCAGGTTTCCTGAAAGGCGGGAGT
  CAGACCTCCGCCCCCAGCCAGAGCAAGCTTGGGGCACCATGCCCAGGAGGGAAGAAGCCA
  TCCACAGCCTTCCCTGTCACCGGCTCCTCTGTCCTGCCTACCCTGGTCCTGGCGGGACTT
  CACTATTTGACTTGGTTTCCTTTCAGATATTCTTGGCTCAGGGCCTGGGTTGAGGGAGCT
  TAGGGAAGGACGTCCGTCTGGGTGCTTTTCCTCCAGTTTGCTGGCTGGCTTCTCCGTCTA
  CCCACAGTGACCTCACAGAGAGGCCCTCCTGCCACCCATGCTCATGTGGTGTCCCCACCG
  CCCACTTGTTTGATGTCACTGACTGTCTACATGTATTTATATTCTTGATATTTTCTACCC
  TCACTAGAATGTAAACTCCATGAAGGCACAGACTTTTCTTGTTCTCTTCTCTATCCCTAG
  AGTAAGACCAACTTGAACCTGGCATATAGTAGCTGCTTAATAAATACTCGTCTGTCAATG
  AAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006319
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006319.3, NP_006310.1
• RefSeq Size: 1874 bp
• RefSeq ORF: 642 bp
• Locus ID: 10423
• UniProt ID: O14735
• Cytogenetics: 16p11.2
• Domains: CDP-OH_P_transf
• Protein Families: Transmembrane
• Protein Pathways: Glycerophospholipid metabolism, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: Phosphatidylinositol breakdown products are ubiquitous second messengers that function downstream of many G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. Two enzymes, CDP-diacylglycerol synthase and phosphatidylinositol synthase, are involved in the biosynthesis of phosphatidylinositol. Phosphatidylinositol synthase, a member of the CDP-alcohol phosphatidyl transferase class-I family, is an integral membrane protein found on the cytoplasmic side of the endoplasmic reticulum and the Golgi apparatus. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).