C 4 Methylsterol Oxidase (MSMO1) (NM_006745) Human Untagged Clone

CAT#: SC319845

MSMO1 (untagged)-Human sterol-C4-methyl oxidase-like (SC4MOL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: C 4 Methylsterol Oxidase
• Synonyms: DESP4; ERG25; MCCPD; SC4MOL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006745.3 GGGGGGAACACCTGGCGAGTCCTCGGTGTCGGTGGCCGGCAGTCATCTCGCGGCCGTTCA
  GAATTATAAGGCTGTCTGCAGAGATTTGAAAAATGGCAACAAATGAAAGTGTCAGCATCT
  TTAGTTCAGCATCCTTGGCTGTGGAATATGTAGATTCACTTTTACCTGAGAATCCTCTGC
  AAGAACCATTTAAAAATGCTTGGAACTATATGTTGAATAATTATACAAAGTTCCAGATTG
  CAACATGGGGATCCCTTATAGTTCATGAAGCCCTTTATTTCTTATTCTGTTTACCTGGAT
  TTTTATTTCAATTTATACCTTATATGAAAAAATACAAAATTCAAAAGGATAAGCCAGAGA
  CATGGGAAAACCAATGGAAGTGTTTCAAAGTTCTTCTCTTTAATCACTTCTGTATCCAGC
  TGCCTTTGATTTGTGGAACCTATTATTTTACAGAGTATTTCAATATTCCTTATGATTGGG
  AAAGAATGCCAAGATGGTATTTTCTTTTGGCAAGATGCTTTGGTTGTGCAGTCATTGAAG
  ATACTTGGCACTATTTTCTGCATAGACTCTTACACCACAAAAGAATATACAAGTATATTC
  ATAAAGTTCATCATGAGTTTCAGGCTCCATTTGGAATGGAAGCTGAATATGCACATCCTT
  TGGAGACTCTAATTCTTGGAACTGGATTTTTCATTGGAATCGTGCTTTTGTGTGATCATG
  TAATTCTTCTTTGGGCATGGGTGACCATTCGTTTATTAGAAACTATTGATGTCCATAGTG
  GTTATGATATTCCTCTCAACCCTTTAAATCTGATCCCTTTCTATGCTGGTTCTCGGCATC
  ATGATTTCCACCACATGAACTTCATTGGAAACTATGCTTCAACATTTACATGGTGGGATC
  GAATTTTTGGAACAGACTCTCAGTATAATGCCTATAATGAAAAGAGGAAGAAGTTTGAGA
  AAAAGACTGAATAAATATCTCACGTAAACCTTCCTGAAAGATAAACGTTTTCCTGAATTC
  AGAAACTAGTAGCTAACATTGCTTCTGGAGAGCAGAAATAAGCATGTCTTCTGGCTACTA
  AGTGATAAGAAGAACATTAACAACCTTTAATTACCTTCCTAGTGGGAACTTTTTCTACTT
  TACCTACAAGTTCTATATATGTAGAAATGAATAAATATATATTTAAGTACAGTTTTCATG
  AGGAAGTTTTAAAAGGCCATGTTCCTAAGCTTCCAAGAAGGTTTTGGATACTAGAAGTAT
  TAATCTATGGCTTTTCTCCCAGTAAAACCATAGGCCTGAAGTTGACATTGGTCTTTAAAT
  CTTTTAGATATATAGTGGTCATTTCAGAAAATTCTTCATAGTGGTATTGGCCTTATATTT
  AACTTTTTTTTTACTTTTTTTTTTGAGACGAAGCCACACTCTGTCTCCTTGGCTGGAGTG
  TGGTGGCACAGTCTCAGCTCACTGCAACCTCTGCCTCCCAGTTCAAGCAATTCTTCTGCC
  TCAGCCTCCCAAGTAGCTGGGATTACAGGCACCCGCCACCACGCCCAGCTAATTTTTGTA
  TTTTTGTAGAGATGGGGTTTCACGATGTTGGCCAGGCTGGTCTCAAACTTCTGACCTCAA
  GTGATCTGCCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTAAGCCACTGCACCC
  GGCCTTTTTAACTTTAAACATGTTTTAGAATTCGCCTAAAGATCAAAATATCATGGATTG
  AACCTCATCAATTGATAGCAGTGAGTGACTGAAGCTTCCAAATCAAGAAAAGCCAGCACC
  AAGAACTTCCATTCTAATCTAGAGCTGACCAGTTTGAGCTGATTCTCTCTTTGAAGAGTC
  CTTCTTGATTGCAGTGCAGTACTGGCATTTCTGAATGGATGTAAGTGGAGTATTTTAGTC
  TAAAGGCTTTTCAAATTACTTGAATTTTTTTAAAAATTGAGGAGCTTTATTTCTATTTAC
  CCTTCCATTTTTGTATATCAAATTTCCATTGTCATTAAAAACTGTATCTTGAAACTTTGT
  GAACTGACTTGCTGTATTTGCACTTTGAGCTCTTGAAATAAATGTGATTTTTGTGTGATT
  ATCTGGTTTCCAGTTTTAAACATTAACTGTCACCTTTTATTCTTAAACTTGAAAGTACAG
  AAATCATTAAATTATTAAGTTGTACAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006745
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006745.3, NP_006736.1
• RefSeq Size: 2214 bp
• RefSeq ORF: 882 bp
• Locus ID: 6307
• UniProt ID: Q15800
• Cytogenetics: 4q32.3
• Domains: Sterol_desat
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: Sterol-C4-mehtyl oxidase-like protein was isolated based on its similarity to the yeast ERG25 protein. It contains a set of putative metal binding motifs with similarity to that seen in a family of membrane desaturases-hydroxylases. The protein is localized to the endoplasmic reticulum membrane and is believed to function in cholesterol biosynthesis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).