ATF1 (NM_005171) Human Untagged Clone

CAT#: SC319835

ATF1 (untagged)-Human activating transcription factor 1 (ATF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATF1
• Synonyms: EWS-ATF1; FUS/ATF-1; TREB36
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005171.2 GAGGCGGCAGCCACAGTTGATTATGGAAGATTCCCACAAGAGTACCACGTCAGAGACAGC
  ACCTCAACCTGGTTCAGCAGTTCAGGGAGCTCACATTTCTCATATTGCTCAACAGGTATC
  ATCTTTATCAGAAAGTGAGGAGTCCCAGGACTCATCCGACAGCATAGGCTCCTCACAGAA
  AGCCCACGGGATCCTAGCACGGCGCCCATCTTACAGAAAAATTTTGAAAGACTTATCTTC
  TGAAGATACACGGGGCAGAAAAGGAGACGGAGAAAATTCTGGAGTTTCTGCTGCTGTCAC
  TTCTATGTCTGTTCCAACTCCCATCTATCAGACTAGCAGCGGACAGTATATTGCCATTGC
  CCCAAATGGAGCCTTACAGTTGGCAAGTCCAGGCACAGATGGAGTACAGGGACTTCAGAC
  ATTAACCATGACAAATTCAGGCAGTACTCAGCAAGGTACAACTATTCTTCAGTATGCACA
  GACCTCTGATGGACAGCAGATACTTGTGCCCAGCAATCAGGTGGTCGTACAAACTGCATC
  AGGAGATATGCAAACATATCAGATCCGAACTACACCTTCAGCTACTTCTCTGCCACAAAC
  TGTGGTGATGACATCTCCTGTGACTCTCACCTCTCAGACAACTAAGACAGATGACCCCCA
  ATTGAAAAGAGAAATAAGGTTAATGAAAAACAGAGAAGCTGCTCGAGAATGTCGCAGAAA
  GAAGAAAGAATATGTGAAATGCCTGGAAAACCGAGTTGCAGTCCTGGAAAATCAAAATAA
  AACTCTAATAGAAGAGTTAAAAACTTTGAAGGATCTTTATTCCAATAAAAGTGTTTGATT
  CCTAAGAAAGAAAATATTTTTGTGGACATGCATAAAAATTAAATGGATTTCCTAGTGGAG
  TTTTATAAATTAAAAGGTCAAAACTGAAGCTTTTTATTTAGGCTTTTCCAAATCAAGGAT
  AAATATCTTACGCACGATATCTAGTGACAGAGGAGAAAGTGGAAAATGACCTCAAGGAAG
  CTACGGGCACAACTGGAAGCCTTGTAGAAATTAAACATATTCAAGGAGCAAGAAATGAAC
  TTTCAGCAGTCTAAATTTTCTAAATAACCAATAGTTGCCAATCTAAAGTGGCAGAGAAGA
  TGAAATTTGATAAACTGAATTTTTTTTAAAAATCCATTTACCCTACAGGTTTGCATTTGT
  TTGCTGAAATTTACCTTTTTTTAGTTATATATATGTGTGTGTGTGTGTGTAATTTCTGCC
  AATAAATTCTAAATTACAAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005171
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005171.2, NP_005162.1
• RefSeq Size: 1568 bp
• RefSeq ORF: 816 bp
• Locus ID: 466
• UniProt ID: P18846
• Cytogenetics: 12q13.12
• Domains: pKID, BRLZ
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]