MRPS34 (NM_023936) Human Untagged Clone

CAT#: SC319412

MRPS34 (untagged)-Human mitochondrial ribosomal protein S34 (MRPS34), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPS34
• Synonyms: COXPD32; MRP-S12; MRP-S34; MRPS12
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_023936.1 GCTGAGGACGCGGACCCGCCATGGCGCGGAAGAAGGTGCGTCCGCGGCTGATCGCGGAGC
  TGGCCCGCCGCGTGCGCGCCCTGCGGGAGCAACTGAACAGGCCGCGCGACTCCCAGCTCT
  ACGCGGTGGACTACGAGACCTTGACGCGGCCGTTCTCTGGACGCCGGCTGCCGGTCCGGG
  CCTGGGCCGACGTGCGCCGCGAGAGCCGCCTCTTGCAGCTGCTCGGCCGCCTCCCGCTCT
  TCGGCCTGGGCCGCCTGGTCACGCGCAAGTCCTGGCTGTGGCAGCACGACGAGCCGTGCT
  ACTGGCGCCTCACGCGGGTGCGGCCCGACTACACGGCGCAGAACTTGGACCACGGGAAGG
  CCTGGGGCATCCTGACCTTCAAAGGGAAGACTGAGAGCGAGGCGCGGGAGATCGAACACG
  TCATGTACCATGACTGGCGGCTGGTGCCCAAGCACGAGGAGGAGGCCTTCACCGCGTTCA
  CGCCGGCGCCGGAAGACAGCCTGGCCTCCGTGCCGTACCCGCCTCTCCTCCGGGCCATGA
  TTATCGCAGAACGACAGAAAAATGGAGACACAAGCACCGAGGAGCCCATGCTGAATGTGC
  AGAGGATACGCATGGAACCCTGGGATTACCCTGCAAAACAGGAAGACAAAGGAAGGGCCA
  AGGGCACCCCCGTCTAGAATGCCAGAACCAGCGGGTGGCCTTAGGGGCTGTGAGGCAGTG
  GGGACCTTATTGATGAAAGAAACCGTCTTTGCGTTACACCCGAGTCTGCCTCTCGGAGCA
  GGGAGCTCACCTTCCGCGACGTGTTCTGAGGGTCTGCATCTTAGGGGGGAGGGCTGGGGC
  AAATCGCCACCTGTGCCTTTCCTCTGGCCCTGCTGCCCCCACACCCAACTCCGAGGGCCC
  ACGCTGGGGAAAGCGGGAAGCGCTCGCTCCCTTTCCCCCATTAGTGCTCTCTCTGCCTGG
  ATCCCGGCAGAAGCTATGAAAGGGAATAAAGAGAAAAGAAGTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_023936
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023936.1, NP_076425.1
• RefSeq Size: 1020 bp
• RefSeq ORF: 657 bp
• Locus ID: 65993
• UniProt ID: P82930
• Cytogenetics: 16p13.3
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.