PML Protein (PML) (NM_033250) Human Untagged Clone

CAT#: SC319404

PML (untagged)-Human promyelocytic leukemia (PML), transcript variant 11

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PML Protein
• Synonyms: MYL; PP8675; RNF71; TRIM19
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033250.2 CTTCAGCTTCTCTTCACGCACTCCAAGATCTAAACCGAGAATCGAAACTAAGCTGGGGTC
  CATGGAGCCTGCACCCGCCCGATCTCCGAGGCCCCAGCAGGACCCCGCCCGGCCCCAGGA
  GCCCACCATGCCTCCCCCCGAGACCCCCTCTGAAGGCCGCCAGCCCAGCCCCAGCCCCAG
  CCCTACAGAGCGAGCCCCCGCTTCGGAGGAGGAGTTCCAGTTTCTGCGCTGCCAGCAATG
  CCAGGCGGAAGCCAAGTGCCCGAAGCTGCTGCCTTGTCTGCACACGCTGTGCTCAGGATG
  CCTGGAGGCGTCGGGCATGCAGTGCCCCATCTGCCAGGCGCCCTGGCCCCTAGGTGCAGA
  CACACCCGCCCTGGATAACGTCTTTTTCGAGAGTCTGCAGCGGCGCCTGTCGGTGTACCG
  GCAGATTGTGGATGCGCAGGCTGTGTGCACCCGCTGCAAAGAGTCGGCCGACTTCTGGTG
  CTTTGAGTGCGAGCAGCTCCTCTGCGCCAAGTGCTTCGAGGCACACCAGTGGTTCCTCAA
  GCACGAGGCCCGGCCCCTAGCAGAGCTGCGCAACCAGTCGGTGCGTGAGTTCCTGGACGG
  CACCCGCAAGACCAACAACATCTTCTGCTCCAACCCCAACCACCGCACCCCTACGCTGAC
  CAGCATCTACTGCCGAGGATGTTCCAAGCCGCTGTGCTGCTCGTGCGCGCTCCTTGACAG
  CAGCCACAGTGATCTCAAGTGCGACATCAGCGCAGAGATCCAGCAGCGACAGGAGGAGCT
  GGACGCCATGACGCAGGCGCTGCAGGAGCAGGATAGTGCCTTTGGCGCGGTTCACGCGCA
  GATGCACGCGGCTGTCGGCCAGCTGGGCCGCGCGCGTGCCGAGACCGAGGAGCTGATCCG
  CGAGCGCGTGCGCCAGGTGGTAGCTCACGTGCGGGCTCAGGAGCGCGAGCTGCTGGAGGC
  TGTGGACGCGCGGTACCAGCGCGACTACGAGGAGATGGCCAGTCGGCTGGGCCGCCTGGA
  TGCTGTGCTGCAGCGCATCCGCACGGGCAGCGCGCTGGTGCAGAGGATGAAGTGCTACGC
  CTCGGACCAGGAGGTGCTGGACATGCACGGTTTCCTGCGCCAGGCGCTCTGCCGCCTGCG
  CCAGGAGGAGCCCCAGAGCCTGCAAGCTGCCGTGCGCACCGATGGCTTCGACGAGTTCAA
  GGTGCGCCTGCAGGACCTCAGCTCTTGCATCACCCAGGGGAAAGATGCAGCTGTATCCAA
  GAAAGCCAGCCCAGAGGCTGCCAGCACTCCCAGGGACCCTATTGACGTTGACCTGGATGT
  CTCCAATACAACGACAGCCCAGAAGAGGAAGTGCAGCCAGACCCAGTGCCCCAGGAAGGT
  CATCAAGATGGAGTCTGAGGAGGGGAAGGAGGCAAGGTTGGCTCGGAGCTCCCCGGAGCA
  GCCCAGGCCCAGCACCTCCAAGGCAGTCTCACCACCCCACCTGGATGGACCGCCTAGCCC
  CAGGAGCCCCGTCATAGGAAGTGAGGTCTTCCTGCCCAACAGCAACCACGTGGCCAGTGG
  CGCCGGGGAGGCAGAGGAACGCGTTGTGGTGATCAGCAGCTCGGAAGACTCAGATGCCGA
  AAACTCGTGCATGGAGCCCATGGAGACCGCCGAGCCACAGTCCTCGCCAGCCCACTCCTC
  GCCAGCCCACTCCTCGCCAGCCCACTCCTCGCCAGTCCAGTCTCTGCTGAGAGCACAAGG
  AGCCTCCAGCCTGCCCTGTGGCACATACCACCCCCCAGCTTGGCCTCCCCACCAGCCCGC
  TGAGCAGGCTGCCACCCCCGATGCTGAGCCTCACAGCGAGCCTCCTGATCACCAGGAGCG
  CCCTGCCGTCCACCGTGGGATCCGCTACCTGTTGTACAGAGCACAGAGAGCCATCCGCCT
  TCGCCATGCCCTCCGCTTGCACCCTCAATTGCATCGGGCCCCTATTCGGACTTGGTCTCC
  CCATGTGGTCCAAGCCAGCACTCCTGCCATCACAGGGCCCCTCAACCATCCTGCCAATGC
  CCAGGAACATCCTGCCCAGCTGCAAAGGGGCATCAGCCCACCCCACCGGATACGAGGGGC
  TGTGCGATCCCGCAGCCGCTCCCTCCGGGGCTCCTCCCATTTATCCCAGTGGCTCAACAA
  CTTTTTTGCCCTCCCCTTCTCCTCCATGGCTTCCCAGCTTGACATGTCTTCCGTGGTGGG
  GGCAGGGGAAGGCAGAGCCCAGACTCTTGGAGCAGTTGTTCCCCCTGGGGACTCTGTCAG
  AGGCTCCATGGAGGCCTCTCAAGTCCAAGTGCCTCTGGAAGCCTCTCCAATTACATTCCC
  ACCACCCTGTGCCCCAGAAAGGCCCCCCATCAGCCCAGTCCCAGGCGCCCGTCAAGCAGG
  CCTCTGAGAGTGCTACCCTTCTCTTGTAACCTTGCAGCCAACACCCCTGCCCGGCCCCTG
  AGCTGCCTCCTCCAGCCCATGCTCTTACAGGCCCTGCACAGAGTAGCACTCATTAATTCT
  TGGTTAAGGAATGAATCAACGAATGAATGGCTATGCATGGACCTCTGGGCAGGGAGACCT
  GGGTCTTCTCTGGCTGAGAGGGGAAGGCTAAGGCATGGCTGAGATTCAAGCCACCATTCC
  AGGCCTCTTTGCCCAAGAAAGAAACTTCTGTCACCCTTGCACTCTCCTGTGTTCTGAGTC
  CCTGGCCAATAGCACAGCCTTCCATGCCCCGACCCCCACCCCAAGCCTCTCCACTAGGCC
  TCTGCCAGGATCTAAGCCCATGAGCACAGGGACTGGCTATCCCAAGACCTGGCAGATGTG
  GCTGCTCAATAAACACTTGTTGAACCATCAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_033250
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033250.2, NP_150253.2
• RefSeq Size: 2944 bp
• RefSeq ORF: 2346 bp
• Locus ID: 5371
• UniProt ID: P29590
• Cytogenetics: 15q24.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer, Ubiquitin mediated proteolysis
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (11) has multiple differences in the coding region compared to variant 1, one of which results in translational frame-shift. The resulting isoform (11, also known as PML-2A and PML-IIA) has a distinct C-terminus and is shorter than isoform 1.