Cathepsin F (CTSF) (NM_003793) Human Untagged Clone

CAT#: SC319324

CTSF (untagged)-Human cathepsin F (CTSF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cathepsin F
• Synonyms: CATSF; CLN13
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003793.3 CTCAGGCCCCGCTGGCCGCGGGCTCGGTACCCGGTGGGTCGGTGGAGCGTCTGTTGGGTC
  CGGGCCGCCGGCTTCGCCCTCGCCATGGCGCCCTGGCTGCAGCTCCTGTCGCTGCTGGGG
  CTGCTCCCGGGCGCAGTGGCCGCCCCCGCCCAGCCCCGAGCCGCCAGCTTTCAGGCCTGG
  GGGCCGCCGTCCCCGGAGCTGCTGGCGCCCACCCGCTTCGCGCTGGAGATGTTCAACCGC
  GGCCGGGCTGCGGGGACGCGGGCCGTGCTGGGCCTTGTGCGCGGCCGCGTCCGCCGGGCG
  GGCCAGGGGTCGCTGTACTCCCTGGAGGCCACCCTGGAGGAGCCACCCTGCAACGACCCC
  ATGGTGTGCCGGCTCCCCGTGTCCAAGAAAACCCTGCTCTGCAGCTTCCAAGTCCTGGAT
  GAGCTCGGAAGACACGTGCTGCTGCGGAAGGACTGTGGCCCAGTGGACACCAAGGTTCCA
  GGTGCTGGGGAGCCCAAGTCAGCCTTCACTCAGGGCTCAGCCATGATTTCTTCTCTGTCC
  CAAAACCATCCAGACAACAGAAACGAGACTTTCAGCTCAGTCATTTCCCTGTTGAATGAG
  GATCCCCTGTCCCAGGACTTGCCTGTGAAGATGGCTTCAATCTTCAAGAACTTTGTCATT
  ACCTATAACCGGACATATGAGTCAAAGGAAGAAGCCCGGTGGCGCCTGTCCGTCTTTGTC
  AATAACATGGTGCGAGCACAGAAGATCCAGGCCCTGGACCGTGGCACAGCTCAGTATGGA
  GTCACCAAGTTCAGTGATCTCACAGAGGAGGAGTTCCGCACTATCTACCTGAATACTCTC
  CTGAGAAAAGAGCCTGGCAACAAGATGAAGCAAGCCAAGTCTGTGGGTGACCTCGCCCCA
  CCTGAATGGGACTGGAGGAGTAAGGGGGCTGTCACAAAAGTCAAAGACCAGGGCATGTGT
  GGCTCCTGCTGGGCCTTCTCAGTCACAGGCAATGTGGAGGGCCAGTGGTTTCTCAACCAG
  GGGACCCTGCTCTCCCTCTCTGAACAGGAGCTCTTGGACTGTGACAAGATGGACAAGGCC
  TGCATGGGCGGCTTGCCCTCCAATGCCTACTCGGCCATAAAGAATTTGGGAGGGCTGGAG
  ACAGAGGATGACTACAGCTACCAGGGTCACATGCAGTCCTGCAACTTCTCAGCAGAGAAG
  GCCAAGGTCTACATCAATGACTCCGTGGAGCTGAGCCAGAACGAGCAGAAGCTGGCAGCC
  TGGCTGGCCAAGAGAGGCCCAATCTCCGTGGCCATCAATGCCTTTGGCATGCAGTTTTAC
  CGCCACGGGATCTCCCGCCCTCTCCGGCCCCTCTGCAGCCCTTGGCTCATTGACCATGCG
  GTGTTGCTTGTGGGCTACGGCAACCGCTCTGACGTTCCCTTTTGGGCCATCAAGAACAGC
  TGGGGCACTGACTGGGGTGAGAAGGGTTACTACTACTTGCATCGCGGGTCCGGGGCCTGT
  GGCGTGAACACCATGGCCAGCTCGGCGGTGGTGGACTGAAGAGGGGCCCCCAGCTCGGGA
  CCTGGTGCTGATCAGAGTGGCTGCTGCCCCAGCCTGACATGTGTCCAGGCCCCTCCCCGG
  GAGGTACAGCTGGCAGAGGGAAAGGCACTGGGTACCTCAGGGTGAGCAGAGGGCACTGGG
  CTGGGGCACAGCCCCTGCTTCCCTGCACCCCATTCCCACCCTGAAGTTCTGCACCTGCAC
  CTTTGTTGAATTGTGGTAGCTTAGGAGGATGTCAGGGTGAAGGGTGGTATCTTGGCAGTT
  GAAGCTGGGGCAAGAACTCTGGGCTTGGGTAATGAGCAGGAAGAAAATTTTCTGATCTTA
  AGCCCAGCTCTGTTCTGCCCCCGCTTTCCTCTGTTTGATACTATAAATTTTCTGGTTCCC
  TTGGATTTAGGGATAGTGTCCCCCTCCATGTCCAGGAAACTTGTAACCACCCTTTTCTAA
  CAGCAATAAAGAGGTGTCCTTGTCCCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003793
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003793.3, NP_003784.2
• RefSeq Size: 2032 bp
• RefSeq ORF: 1455 bp
• Locus ID: 8722
• UniProt ID: Q9UBX1
• Cytogenetics: 11q13.2
• Domains: Pept_C1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome
• Gene Summary: Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W. [provided by RefSeq, Jul 2008]