LSM14A (NM_001114093) Human Untagged Clone

CAT#: SC318896

LSM14A (untagged)-Human LSM14A, SCD6 homolog A (S. cerevisiae) (LSM14A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LSM14A
• Synonyms: C19orf13; FAM61A; RAP55; RAP55A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318896 representing NM_001114093.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGCGGGGGCACCCCTTACATCGGCAGCAAGATCAGCCTCATCTCCAAGGCGGAGATCCGCTACGAG
  GGCATCCTCTACACCATCGACACCGAAAACTCCACCGTAGCCCTTGCCAAAGTTCGATCCTTTGGTACA
  GAAGACAGACCGACAGATCGTCCAATACCACCTCGAGATGAAGTCTTTGAATACATTATATTCCGTGGG
  AGTGACATTAAAGACCTTACTGTTTGTGAGCCACCAAAACCACAGTGTTCTTTGCCTCAAGACCCAGCT
  ATTGTTCAGTCCTCACTAGGCTCATCGACTTCTTCATTCCAGTCCATGGGTTCTTATGGACCTTTCGGC
  AGGATGCCCACATACAGTCAGTTCAGTCCGAGTTCCTTAGTTGGGCAGCAGTTTGGTGCTGTTGGTGTT
  GCTGGAAGCTCTTTGACATCCTTTGGAACAGAAACATCAAACAGTGGTACCTTACCCCAAAGTAGTGCG
  GTTGGTTCTGCCTTTACACAGGATACAAGATCTCTAAAAACACAGTTATCTCAAGGTCGCTCAAGCCCT
  CAGTTAGACCCTTTGAGAAAAAGCCCAACCATGGAACAAGCAGTGCAGACCGCCTCAGCCCACTTACCT
  GCTCCAGCAGCTGTTGGGAGAAGGAGTCCTGTATCAACCAGGCCTTTGCCATCTGCCAGCCAAAAGGCA
  GGAGAGAATCAGGAGCACAGGCGAGCTGAAGTACACAAAGTTTCAAGGCCAGAAAATGAGCAACTCAGA
  AATGATAACAAGAGACAAGTAGCTCCAGGTGCTCCTTCAGCTCCAAGGAGAGGGCGTGGGGGTCATCGG
  GGTGGCAGGGGAAGATTTGGTATTCGGCGAGATGGGCCAATGAAATTTGAGAAAGACTTTGACTTTGAA
  AGTGCAAATGCACAATTCAACAAGGAAGAGATTGACAGAGAGTTTCATAATAAACTTAAATTAAAAGAA
  GATAAACTTGAGAAACAGGAGAAGCCTGTAAATGGTGAAGATAAAGGAGACTCAGGAGTTGATACCCAA
  AACAGTGAAGGAAATGCCGATGAAGAAGATCCACTTGGACCTAATTGCTATTATGACAAAACTAAATCC
  TTCTTTGATAATATTTCTTGTGATGACAATAGAGAACGGAGACCAACCTGGGCTGAAGAAAGAAGATTA
  AATGCTGAAACATTTGGAATCCCACTTCGTCCAAACCGTGGCCGTGGGGGATACAGAGGCAGAGGAGGT
  CTTGGTTTCCGTGGTGGCAGAGGGCGTGGTGGTGGCAGAGGTGGTACCTTCACTGCCCCTCGAGGATTT
  CGCGGTGGATTCAGAGGAGGTCGTGGGGGCCGGGAGTTTGCGGATTTTGAATATAGGAAAACCACAGCT
  TTTGGACCCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001114093
• Insert Size: 1392 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114093.1
• RefSeq Size: 3772 bp
• RefSeq ORF: 1392 bp
• Locus ID: 26065
• UniProt ID: Q8ND56
• Cytogenetics: 19q13.11
• MW: 50.5 kDa
• Gene Summary: Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes isoform a. While isoforms a and b are of the same length, their C-termini are different. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.