HIF3 alpha (HIF3A) (NM_152795) Human Untagged Clone

CAT#: SC318135

HIF3A (untagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIF3 alpha
• Synonyms: bHLHe17; HIF-3A; HIF3-alpha-1; IPAS; MOP7; PASD7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_152795 edited
  CCGAGGGCTCCGGAGCGGCGACTGGCGAGCCATGGCGCTGGGGCTGCAGCGCGCAAGGTC
  GACCACGGAGCTGCGCAAGGAAAAGTCCCGGGATGCGGCCCGCAGCCGGCGCAGCCAGGA
  GACCGAGGTGCTGTACCAGCTGGCTCACACGCTGCCCTTCGCCCGCGGCGTCAGCGCCCA
  CCTGGACAAGGCCTCTATCATGCGCCTCACCATCAGCTACCTGCGCATGCACCGCCTCTG
  CGCCGCAGGGGAGTGGAACCAGGTGGGAGCAGGGGGAGAACCACTGGATGCCTGCTACCT
  GAAGGCCCTGGAGGGCTTCGTCATGGTGCTCACCGCCGAGGGAGACATGGCTTACCTGTC
  GGAGAATGTCAGCAAACACCTGGGCCTCAGTCAGCTGGAGCTCATTGGACACAGCATCTT
  TGATTTCATCCACCCCTGTGACCAAGAGGAGCTTCAGGACGCCCTGACCCCCCAGCAGAC
  CCTGTCCAGGAGGAAGGTGGAGGCCCCCACGGAGCGGTGCTTCTCCTTGCGCATGAAGAG
  TACGCTCACCAGCCGCGGGCGCACCCTCAACCTCAAGGCGGCCACCTGGAAGGTGCTGAA
  CTGCTCTGGACATATGAGGGCCTACAAGCCACCTGCGCAGACTTCTCCAGCTGGGAGCCC
  TGACTCAGAGCCCCCGCTGCAGTGCCTGGTGCTCATCTGCGAAGCCATCCCCCACCCAGG
  CAGCCTGGAGCCCCCACTGGGCCGAGGGGCCTTCCTCAGCCGCCACAGCCTGGACATGAA
  GTTCACCTACTGTGACGACAGGATTGCAGAAGTGGCTGGCTATAGTCCCGATGACCTGAT
  CGGCTGTTCCGCCTACGAGTACATCCACGCGCTGGACTCCGATGCGGTCAGCAAGAGCAT
  CCACACCTTGCTGAGCAAGGGCCAGGCAGTAACAGGGCAGTATCGCTTCCTGGCCCGGAG
  TGGTGGCTACCTGTGGACCCAGACCCAGGCCACAGTGGTGTCAGGGGGACGGGGCCCCCA
  GTCGGAGAGTATCGTCTGTGTCCATTTTTTAATCAGCCAGGTGGAAGAGACCGGAGTGGT
  GCTGTCCCTGGAGCAAACGGAGCAACACTCTCGCAGACCCATTCAGCGGGGCGCCCCCTC
  TCAGAAGGACACCCCTAACCCTGGGGACAGCCTTGACACCCCTGGCCCCCGGATCCTTGC
  CTTCCTGCACCCGCCTTCCCTGAGCGAGGCTGCCCTGGCCGCTGACCCCCGCCGTTTCTG
  CAGCCCTGACCTCCGTCGCCTCCTGGGACCCATCCTGGATGGGGCTTCAGTAGCAGCCAC
  TCCCAGCACCCCGCTGGCCACACGGCACCCCCAAAGTCCTCTTTCGGCTGATCTCCCAGA
  TGAACTACCTGTGGGCACCGAGAATGTGCACAGACTCTTCACCTCCGGGAAAGACACTGA
  GGCAGTGGAGACAGATTTAGATATAGCTCAGGATGCTGATGCTCTGGATTTGGAGATGCT
  GGCCCCCTACATCTCCATGGATGATGACTTCCAGCTCAACGCCAGCGAGCAGCTACCCAG
  GGCCTACCACAGACCTCTGGGGGCTGTCCCCCGGCCCCGTGCTCGGAGCTTCCATGGCCT
  GTCACCTCCAGCCCTTGAGCCCTCCCTGCTACCCCGCTGGGGGAGTGACCCCCGGCTGAG
  CTGCTCCAGCCCTTCCAGAGGGGACCCCTCAGCATCCTCTCCCATGGCTGGGGCTCGGAA
  GAGGACCCTGGCCCAGAGCTCAGAGGACGAGGACGAGGGAGTGGAGCTGCTGGGAGTGAG
  ACCTCCCAAAAGGTCCCCCAGCCCAGAACACGAAAACTTTCTGCTCTTTCCTCTCAGCCT
  GAGTTTCCTTCTGACAGGAGGACCAGCCCCAGGGAGCCTGCAGGACCCCAGCACCCCACT
  CCTGAACCTGAATGAGCCCCTGGGCCTGGGCCCCTCACTGCTCTCTCCGTACTCAGACGA
  GGACACTACCCAGCCCGGGGGCCCCTTCCAGCCAAGGGCAGGCTCAGCCCAGGCTGACTG
  AGCCGGCTCCTCTCCCCATCTGCCTTCTCCTCCCCCAGAAAGGACCTCAACCACACTCCA
  CGCCGGCAGCCAACGCACAGGATGGGGGCGCCAGGAGAGGGGCCCCTCTCTCCTCTATGT
  ACCCCCTGCCCACCTCGGGCCTACCTCAGCCCTCACCCCTCTGCCTGCTCCCAATCTGGG
  GGCTCTCTGGGGTGGTCTCAGCTCAGTGACCTCTGGGAGGTGGTCCCTGGCCCCCTCCTC
  CTTCTCTCAGGATTTCTCTTGGGGTTCTCAATACTTGGTTACCTCATTATCCCTTTCTCT
  GCCTCTCTTGGCTTTATTTTGGGGAATCAGGGGTGAGGAGGGTTGGGGGGGTCATATCTG
  TGTTTCCAGGTTCTGGGGAGAACAATGATCCACGGGTCAACGTGATCACATTTCCTTCTA
  AAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152795 unedited
  GCATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGCCGAGGGCTC
  CGGAGCGGCGACTGGCGAGCCATGGCGCTGGGGCTGCAGCGCGCAAGGTCGACCACGGAG
  CTGCGCAAGGAAAAGTCCCGGGATGCGGCCCGCAGCCGGCGCAGCCAGGAGACCGAGGTG
  CTGTACCAGCTGGCTCACACGCTGCCCTTCGCCCGCGGCGTCAGCGCCCACCTGGACAAG
  GCCTCTATCATGCGCCTCACCATCAGCTACCTGCGCATGCACCGCCTCTGCGCCGCAGGG
  GAGTGGAACCAGGTGGGAGCAGGGGGAGAACCACTGGATGCCTGCTACCTGAAGGCCCTG
  GAGGGCTTCGTCATGGTGCTCACCGCCGAGGGAGACATGGCTTACCTGTCGGAGAATGTC
  AGCAAACACCTGGGCCTCAGTCAGCTGGAGCTCATTGGACACAGCATCTTTGATTTCATC
  CACCCCTGTGACCAAGAGGAGCTTCAGGACGCCCTGACCCCCCAGCAGACCCTGTCCAGG
  AGGAAGGTGGAGGCCCCCACGGAGCGGTGCTTCTCCTTGCGCATGAAGAGTACGCTCACC
  AGCCGCGGGCGCACCCTCAACCTCAAGGCGGCCACCTGGAAGGTGCTGAACTGCTCTGGA
  CATATGAGGGCCTACAAGCCACCTGCGCAGACTTCTCCAGCTGGGAGCCCTGACTCAGAG
  CCCCCGCTGCAGTGCCTGGTGCTCATCTGCGAAGCCATCCCCCACCCAGGCAGCCTGGAG
  CCCCCACTGGGGCCGAGGGCCTTCCTCAGCCGCCCAGCCTGACATGAAGTCACCTACTGT
  GACACAGGATTGCAGAAGTGCTGGCTTAGTCCGATGACTGATCGCTGNTCGNCTACAGTA
  CTNCCGCGCTGACTCGATGCGTCAGCAGACATTCACACTGCTGACAGGGCCAGCATACAG
  GCATATCGTTCTGGCCGGAGTGTGGTACTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152795 unedited
  ATGGACCCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTAGAAGGAAATG
  TGATCACGTTGACCCGTGGATCATTGTTCTCCCCANAACCTGGAAACACAGATATGACCC
  CCCCAACCCTCCTCACCCCTGATTCCCCAAAATAAAGCCAAGAGAGGCAGAGAAAGGGAT
  AATGAGGTAACCAAGTATTGAGAACCCCAAGAGAAATCCTGAGAGAAGGAGGAGGGGGCC
  AGGGACCACCTCCCAGAGGTCACTGAGCTGAGACCACCCCAGAGAGCCCCCAGATTGGGA
  GCAGGCAGAGGGGTGAGGGCTGAGGTAGGCCCGAGGTGGGCAGGGGGTACATAGAGGAGA
  GAGGGGCCCCTCTCCTGGCGCCCCCATCCTGTGCGTTGGCTGCCGGCGTGGAGTGTGGTT
  GAGGTCCTTTCTGGGGGAGGAGAAGGCAGATGGGGAGAGGAGCCGGCTCAGTCAGCCTGG
  GCTGAGCCTGCCCTTGGCTGGAAGGGGCCCCCGGGCTGGGTAGTGTCCTCGTCTGAGTAC
  GGAGAGAGCAGTGAGGGGCCCAGGCCCAGGGGCTCATTCAGGTTCAGGAGTGGGGTGCTG
  GGGTCCTGCAGGCTCCCTGGGGCTGGTCCTCCTGTCAGAAGGAAACTCAGGCTGAGAGGA
  AAGAGCAGAAAGTTTTCGTGTTCTGGGCTGGGGGACCTTTTGGGAGGTCTCACTCCCAGC
  AGCTCCACTCCCTCGTCCTCGTCCTCTGAGCTCTGGGCCAGGGTCCTCTTCCGAGCCCCA
  NCCATGGGAGAGGATGCTGAGGGGTCCCCTCTGGGAGGNCTGGAGCAGCTCANCCCGGGG
  TCACTCCCCAGCGGGGTAGCAGGGNAGGGCTCAGGGGCTGNAGTGACAAGCCATGGAAGC
  TCCGAGCACGGGGCGGGGGGACAGCCCCANAGGTCTGTGGGAAGCCCTGGGTAGCTGCTC
  NCTTGCCTTGAGCTGGAAGTCTCATTCATGGAGATTAGGGGGGCCGCATCTCC
  
• Restriction Sites: Please inquire
• ACCN: NM_152795
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152795.2, NP_690008.2
• RefSeq Size: 5850 bp
• RefSeq ORF: 2010 bp
• Locus ID: 64344
• UniProt ID: Q9Y2N7
• Cytogenetics: 19q13.32
• Domains: PAS, HLH
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (3) encodes the longest isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.