HIF3 alpha (HIF3A) (NM_152795) Human Tagged ORF Clone

CAT#: RC210255

HIF3A (Myc-DDK-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 3

Product Images

Circular map for RC210255

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HIF3A (Cat# RC210255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIF3A(Cat# TA800720). Positive lysates LY407285 (100ug) and LC407285 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY407285) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210255 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells were transfected with the pCMV6-HIF1A (Cat# RC202461), pCMV6-EPAS1 (Cat# RC208604) and pCMV6-HIF3A (Cat# RC210255) (1:2000).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HIF3 alpha
• Synonyms: bHLHe17; HIF-3A; HIF3-alpha-1; IPAS; MOP7; PASD7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210255 representing NM_152795
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGGGGCTGCAGCGCGCAAGGTCGACCACGGAGCTGCGCAAGGAAAAGTCCCGGGATGCGGCCC
  GCAGCCGGCGCAGCCAGGAGACCGAGGTGCTGTACCAGCTGGCTCACACGCTGCCCTTCGCCCGCGGCGT
  CAGCGCCCACCTGGACAAGGCCTCTATCATGCGCCTCACCATCAGCTACCTGCGCATGCACCGCCTCTGC
  GCCGCAGGGGAGTGGAACCAGGTGGGAGCAGGGGGAGAACCACTGGATGCCTGCTACCTGAAGGCCCTGG
  AGGGCTTCGTCATGGTGCTCACCGCCGAGGGAGACATGGCTTACCTGTCGGAGAATGTCAGCAAACACCT
  GGGCCTCAGTCAGCTGGAGCTCATTGGACACAGCATCTTTGATTTCATCCACCCCTGTGACCAAGAGGAG
  CTTCAGGACGCCCTGACCCCCCAGCAGACCCTGTCCAGGAGGAAGGTGGAGGCCCCCACGGAGCGGTGCT
  TCTCCTTGCGCATGAAGAGTACGCTCACCAGCCGCGGGCGCACCCTCAACCTCAAGGCGGCCACCTGGAA
  GGTGCTGAACTGCTCTGGACATATGAGGGCCTACAAGCCACCTGCGCAGACTTCTCCAGCTGGGAGCCCT
  GACTCAGAGCCCCCGCTGCAGTGCCTGGTGCTCATCTGCGAAGCCATCCCCCACCCAGGCAGCCTGGAGC
  CCCCACTGGGCCGAGGGGCCTTCCTCAGCCGCCACAGCCTGGACATGAAGTTCACCTACTGTGACGACAG
  GATTGCAGAAGTGGCTGGCTATAGTCCCGATGACCTGATCGGCTGTTCCGCCTACGAGTACATCCACGCG
  CTGGACTCCGATGCGGTCAGCAAGAGCATCCACACCTTGCTGAGCAAGGGCCAGGCAGTAACAGGGCAGT
  ATCGCTTCCTGGCCCGGAGTGGTGGCTACCTGTGGACCCAGACCCAGGCCACAGTGGTGTCAGGGGGACG
  GGGCCCCCAGTCGGAGAGTATCGTCTGTGTCCATTTTTTAATCAGCCAGGTGGAAGAGACCGGAGTGGTG
  CTGTCCCTGGAGCAAACGGAGCAACACTCTCGCAGACCCATTCAGCGGGGCGCCCCCTCTCAGAAGGACA
  CCCCTAACCCTGGGGACAGCCTTGACACCCCTGGCCCCCGGATCCTTGCCTTCCTGCACCCGCCTTCCCT
  GAGCGAGGCTGCCCTGGCCGCTGACCCCCGCCGTTTCTGCAGCCCTGACCTCCGTCGCCTCCTGGGACCC
  ATCCTGGATGGGGCTTCAGTAGCAGCCACTCCCAGCACCCCGCTGGCCACACGGCACCCCCAAAGTCCTC
  TTTCGGCTGATCTCCCAGATGAACTACCTGTGGGCACCGAGAATGTGCACAGACTCTTCACCTCCGGGAA
  AGACACTGAGGCAGTGGAGACAGATTTAGATATAGCTCAGGATGCTGATGCTCTGGATTTGGAGATGCTG
  GCCCCCTACATCTCCATGGATGATGACTTCCAGCTCAACGCCAGCGAGCAGCTACCCAGGGCCTACCACA
  GACCTCTGGGGGCTGTCCCCCGGCCCCGTGCTCGGAGCTTCCATGGCCTGTCACCTCCAGCCCTTGAGCC
  CTCCCTGCTACCCCGCTGGGGGAGTGACCCCCGGCTGAGCTGCTCCAGCCCTTCCAGAGGGGACCCCTCA
  GCATCCTCTCCCATGGCTGGGGCTCGGAAGAGGACCCTGGCCCAGAGCTCAGAGGACGAGGACGAGGGAG
  TGGAGCTGCTGGGAGTGAGACCTCCCAAAAGGTCCCCCAGCCCAGAACACGAAAACTTTCTGCTCTTTCC
  TCTCAGCCTGAGTTTCCTTCTGACAGGAGGACCAGCCCCAGGGAGCCTGCAGGACCCCAGCACCCCACTC
  CTGAACCTGAATGAGCCCCTGGGCCTGGGCCCCTCACTGCTCTCTCCGTACTCAGACGAGGACACTACCC
  AGCCCGGGGGCCCCTTCCAGCCAAGGGCAGGCTCAGCCCAGGCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210255 representing NM_152795
  Red=Cloning site Green=Tags(s)
  
  MALGLQRARSTTELRKEKSRDAARSRRSQETEVLYQLAHTLPFARGVSAHLDKASIMRLTISYLRMHRLC
  AAGEWNQVGAGGEPLDACYLKALEGFVMVLTAEGDMAYLSENVSKHLGLSQLELIGHSIFDFIHPCDQEE
  LQDALTPQQTLSRRKVEAPTERCFSLRMKSTLTSRGRTLNLKAATWKVLNCSGHMRAYKPPAQTSPAGSP
  DSEPPLQCLVLICEAIPHPGSLEPPLGRGAFLSRHSLDMKFTYCDDRIAEVAGYSPDDLIGCSAYEYIHA
  LDSDAVSKSIHTLLSKGQAVTGQYRFLARSGGYLWTQTQATVVSGGRGPQSESIVCVHFLISQVEETGVV
  LSLEQTEQHSRRPIQRGAPSQKDTPNPGDSLDTPGPRILAFLHPPSLSEAALAADPRRFCSPDLRRLLGP
  ILDGASVAATPSTPLATRHPQSPLSADLPDELPVGTENVHRLFTSGKDTEAVETDLDIAQDADALDLEML
  APYISMDDDFQLNASEQLPRAYHRPLGAVPRPRARSFHGLSPPALEPSLLPRWGSDPRLSCSSPSRGDPS
  ASSPMAGARKRTLAQSSEDEDEGVELLGVRPPKRSPSPEHENFLLFPLSLSFLLTGGPAPGSLQDPSTPL
  LNLNEPLGLGPSLLSPYSDEDTTQPGGPFQPRAGSAQAD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152795
• ORF Size: 2007 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152795.4
• RefSeq Size: 5850 bp
• RefSeq ORF: 2010 bp
• Locus ID: 64344
• UniProt ID: Q9Y2N7
• Cytogenetics: 19q13.32
• Domains: PAS, HLH
• Protein Families: Druggable Genome, Transcription Factors
• MW: 72.3 kDa
• Gene Summary: The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]