ORAI1 (NM_032790) Human Untagged Clone

CAT#: SC316298

ORAI1 (untagged)-Human ORAI calcium release-activated calcium modulator 1 (ORAI1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ORAI1
• Synonyms: CRACM1; IMD9; ORAT1; TAM2; TMEM142A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032790 edited
  CCGGCGGAGGTGCCTCGCGGCGCCCGGGCCGGCCCGCGCCTCGGCGGCGTGCTCCATGCA
  TCCGGAGCCCGCCCCGCCCCCGAGCCGTAGCAGTCCCGAGCTTCCCCCAAGCGGCGGCAG
  CACCACCAGCGGCAGCCGCCGGAGCCGCCGCCGCAGCGGGGACGGGGAGCCCCCGGGGGC
  CCCGCCACCGCCGCCGTCCGCCGTCACCTACCCGGACTGGATCGGCCAGAGTTACTCCGA
  GGTGATGAGCCTCAACGAGCACTCCATGCAGGCGCTGTCCTGGCGCAAGCTCTACTTGAG
  CCGCGCCAAGCTTAAAGCCTCCAGCCGGACCTCGGCTCTGCTCTCCGGCTTCGCCATGGT
  GGCAATGGTGGAGGTGCAGCTGGACGCTGACCACGACTACCCACCGGGGCTGCTCATCGC
  CTTCAGTGCCTGCACCACAGTGCTGGTGGCTGTGCACCTGTTTGCGCTCATGATCAGCAC
  CTGCATCCTGCCCAACATCGAGGCGGTGAGCAACGTGCACAATCTCAACTCGGTCAAGGA
  GTCCCCCCATGAGCGCATGCACCGCCACATCGAGCTGGCCTGGGCCTTCTCCACCGTCAT
  TGGCACGCTGCTCTTCCTAGCTGAGGTGGTGCTGCTCTGCTGGGTCAAGTTCTTGCCCCT
  CAAGAAGCAGCCAGGCCAGCCAAGGCCCACCAGCAAGCCCCCCGCCAGTGGCGCAGCAGC
  CAACGTCAGCACCAGCGGCATCACCCCGGGCCAGGCAGCTGCCATCGCCTCGACCACCAT
  CATGGTGCCCTTCGGCCTGATCTTTATCGTCTTCGCCGTCCACTTCTACCGCTCACTGGT
  TAGCCATAAGACCGACCGACAGTTCCAGGAGCTCAACGAGCTGGCGGAGTTTGCCCGCTT
  ACAGGACCAGCTGGACCACAGAGGGGACCACCCCCTGACGCCCGGCAGCCACTATGCCTA
  GGCCCATGTGGTCTGGGCCCTTCCAGTGCTTTGGCCTTACGCCCTTCCCCTTGACCTTGT
  CCTGCCCCAGCCTCACGGACAGCCTGCGCAGGGGGCTGGGCTTCAGCAAGGGGCAGAGCA
  TGGAGGGAAGAGGATTTTTTATAAGAGAAATTTCTGCACTTTGAAAATGTCCTCTAAGAG
  AATAAGCATTTCCTGTTCTTCCAGCTCCAGGTCCACCTCCTGTTGGGAGGCGGTGGGGGG
  CCAAAGTGGGGCCACACACTCGCTGTGTCCCCTCTCCTCCCCTGTGCCAGTGCCACCTGG
  GTGCCTCCTCCTGTCCTGTCCGTCTCAACCTCCCTCCCGTCCAGCATTGAGTGTGTACAT
  GTGTGTGTGACACATAAATATACTCATAAGGACACCTCAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032790 unedited
  TGCGACTTGTATACGACTCCTATAGGGCGGCCGCGTCTCATCAGTATTCTGGTACCGGTC
  CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCCGGCGGAGGTGCCTCGCGGCGCCC
  GGGCCGGCCCGCGCCTCGGCGGCGTGCTCCATGCATCCGGAGCCCGCCCCGCCCCCGAGC
  CGTAGCAGTCCCGAGCTTCCCCCAAGCGGCGGCAGCACCACCAGCGGCAGCCGCCGGAGC
  CGCCGCCGCAGCGGGGACGGGGAGCCCCCGGGGGCCCCGCCACCGCCGCCGTCCGCCGTC
  ACCTACCCGGACTGGATCGGCCAGAGTTACTCCGAGGTGATGAGCCTCAACGAGCACTCC
  ATGCAGGCGCTGTCCTGGCGCAAGCTCTACTTGAGCCGCGCCAAGCTTAAAGCCTCCAGC
  CGGACCTCGGCTCTGCTCTCCGGCTTCGCCATGGTGGCAATGGTGGAGGTGCAGCTGGAC
  GCTGACCACGACTACCCACCGGGGCTGCTCATCGCCTTCAGTGCCTGCACCACAGTGCTG
  GTGGCTGTGCACCTGTTTGCGCTCATGATCAGCACCTGCATCCTGCCCAACATCGAGGCG
  GTGAGCAACGTGCACAATCTCAACTCGGTCAAGGAGTCCCCCCATGAGCGCATGCACCGC
  CACATCGAGCTGGCCTGGGCCTTCTCCACCGTCATTGGCACGCTGCTCTTCCTAGCTGAG
  GTGGTGCTGCTCTGCTGGGTCAAGTTCTTGCCCCTCAAGAAGCAGCCAGGCCAGCCAAGG
  CCCACCAGCAAGCCCCCCGCCAGTGGCGCAGCAGCCAACGTCAGCACCAGCGGCATCACC
  CCGGGCCAGGCAGCTGCCATCGCCTCGACCACCATCATGGTGCCCTTCGGCCTGATTTTT
  TATCGTCTTCGC
  
• Restriction Sites: Please inquire
• ACCN: NM_032790
• Insert Size: 1400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032790.2, NP_116179.2
• RefSeq Size: 1497 bp
• RefSeq ORF: 906 bp
• Locus ID: 84876
• UniProt ID: Q96D31
• Cytogenetics: 12q24.31
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1). [provided by RefSeq, Sep 2011]