DPYD (NM_000110) Human Untagged Clone

CAT#: SC316297

DPYD (untagged)-Human dihydropyrimidine dehydrogenase (DPYD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DPYD
• Synonyms: DHP; DHPDHASE; DPD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000110 edited
  TGAGGACGCAAGGAGGGTTTGTCACTGGCAGACTCGAGACTGTAGGCACTGCCATGGCCC
  CTGTGCTCAGTAAGGACTCGGCGGACATCGAGAGTATCCTGGCTTTAAATCCTCGAACAC
  AAACTCATGCAACTCTGTGTTCCACTTCGGCCAAGAAATTAGACAAGAAACATTGGAAAA
  GAAATCCTGATAAGAACTGCTTTAATTGTGAGAAGCTGGAGAATAATTTTGATGACATCA
  AGCACACGACTCTTGGTGAGCGAGGAGCTCTCCGAGAAGCAATGAGATGCCTGAAATGTG
  CAGATGCCCCGTGTCAGAAGAGCTGTCCAACTAATCTTGATATTAAATCATTCATCACAA
  GTATTGCAAACAAGAACTATTATGGAGCTGCTAAGATGATATTTTCTGACAACCCACTTG
  GTCTGACTTGTGGAATGGTATGTCCAACCTCTGATCTTTGTGTAGGTGGATGCAATTTAT
  ATGCCACTGAAGAGGGACCCATTAATATTGGTGGATTGCAGCAATTTGCTACTGAGGTAT
  TCAAAGCAATGAGTATCCCACAGATCAGAAATCCTTCGCTGCCTCCCCCAGAAAAAATGT
  CTGAAGCCTATTCTGCAAAGATTGCTCTTTTTGGTGCTGGGCCTGCAAGTATAAGTTGTG
  CTTCCTTTTTGGCTCGATTGGGGTACTCTGACATCACTATATTTGAAAAACAAGAATATG
  TTGGTGGTTTAAGTACTTCTGAAATTCCTCAGTTCCGGCTGCCGTATGATGTAGTGAATT
  TTGAGATTGAGCTAATGAAGGACCTTGGTGTAAAGATAATTTGCGGTAAAAGCCTTTCAG
  TGAATGAAATGACTCTTAGCACTTTGAAAGAAAAAGGCTACAAAGCTGCTTTCATTGGAA
  TAGGTTTGCCAGAACCCAATAAAGATGCCATCTTCCAAGGCCTGACGCAGGACCAGGGGT
  TTTATACATCCAAAGACTTTTTGCCACTTGTAGCCAAAGGCAGTAAAGCAGGAATGTGCG
  CCTGTCACTCTCCATTGCCATCGATACGGGGAGTCGTGATTGTACTTGGAGCTGGAGACA
  CTGCCTTTGACTGTGCAACATCTGCTCTACGTTGTGGAGCTCGCCGTGTGTTCATCGTCT
  TCAGAAAAGGCTTTGTTAATATAAGAGCTGTCCCTGAGGAGATGGAACTTGCTAAGGAAG
  AAAAGTGTGAATTTCTGCCATTCCTGTCCCCACGGAAGGTTATAGTAAAAGGTGGGAGAA
  TTGTTGCTATGCAGTTTGTTCGGACAGAGCAAGATGAAACTGGAAAATGGAATGAAGATG
  AAGATCAGATGGTCCATCTGAAAGCCGATGTGGTCATCAGTGCCTTTGGTTCAGTTCTGA
  GTGATCCTAAAGTAAAAGAAGCCTTGAGCCCTATAAAATTTAACAGATGGGGTCTCCCAG
  AAGTAGATCCAGAAACTATGCAAACTAGTGAAGCATGGGTATTTGCAGGTGGTGATGTCG
  TTGGTTTGGCTAACACTACAGTGGAATCGGTGAATGATGGAAAGCAAGCTTCTTGGTACA
  TTCACAAATACGTACAGTCACAATATGGAGCTTCCGTTTCTGCCAAGCCTGAACTACCCC
  TCTTTTACACTCCTATTGATCTGGTGGACATTAGTGTAGAAATGGCCGGATTGAAGTTTG
  TAAATCCTTTTGGTCTTGCTAGCGCAACTCCAGCCACCAGCACATCAATGATTCGAAGAG
  CTTTTGAAGCTGGATGGGGTTTTGCCCTCACCAAAACTTTCTCTCTTGATAAGGACATTG
  TGACAAATGTTTCCCCCAGAATCATCCGGGGAACCACCTCTGGCCCCATGTATGGCCCTG
  GACAAAGCTCCTTTCTGAATATTGAGCTCATCAGTGAGAAAACGGCTGCATATTGGTGTC
  AAAGTGTCACTGAACTAAAGGCTGACTTTCCAGACAACATTGTGATTGCTAGCATTATGT
  GCAGTTACAATAAAAATGACTGGACGGAACTTGCCAAGAAGTCTGAGGATTCTGGAGCAG
  ATGCCCTGGAGTTAAATTTATCATGTCCACATGGCATGGGAGAAAGAGGAATGGGCCTGG
  CCTGTGGGCAGGATCCAGAGCTGGTGCGGAACATCTGCCGCTGGGTTAGGCAAGCTGTTC
  AGATTCCTTTTTTTGCCAAGCTGACCCCAAATGTCACTGATATTGTGAGCATCGCAAGAG
  CTGCAAAGGAAGGTGGTGCCAATGGCGTTACAGCCACCAACACTGTCTCAGGTCTGATGG
  GATTAAAATCTGATGGCACACCTTGGCCAGCAGTGGGGATTGCAAAGCGAACTACATATG
  GAGGAGTGTCTGGGACAGCAATCAGACCTATTGCTTTGAGAGCTGTGACCTCCATTGCTC
  GTGCTCTGCCTGGATTTCCCATTTTGGCTACTGGTGGAATTGACTCTGCTGAAAGTGGTC
  TTCAGTTTCTCCATAGTGGTGCTTCCGTCCTCCAGGTATGCAGTGCCATTCAGAATCAGG
  ATTTCACTGTGATCGAAGACTACTGCACTGGCCTCAAAGCCCTGCTTTATCTGAAAAGCA
  TTGAAGAACTACAAGACTGGGATGGACAGAGTCCAGCTACTGTGAGTCACCAGAAAGGGA
  AACCAGTTCCACGTATAGCTGAACTCATGGACAAGAAACTGCCAAGTTTTGGACCTTATC
  TGGAACAGCGCAAGAAAATCATAGCAGAAAACAAGATTAGACTGAAAGAACAAAATGTAG
  CTTTTTCACCACTTAAGAGAAACTGTTTTATCCCCAAAAGGCCTATTCCTACCATCAAGG
  ATGTAATAGGAAAAGCACTGCAGTACCTTGGAACATTTGGTGAATTGAGCAACGTAGAGC
  AAGTTGTGGCTATGATTGATGAAGAAATGTGTATCAACTGTGGTAAATGCTACATGACCT
  GTAATGATTCTGGCTACCAGGCTATACAGTTTGATCCAGAAACCCACCTGCCCACCATAA
  CCGACACTTGTACAGGCTGTACTCTGTGTCTCAGTGTTTGCCCTATTGTCGACTGCATCA
  AAATGGTTTCCAGGACAACACCTTATGAACCAAAGAGAGGCGTACCCTTATCTGTGAATC
  CGGTGTGTTAAGGTGATTTGTGAAACAGTTGCTGTGAACTTTCATGTCACCTACATATGC
  TGATCTTTTAAAATCATGATCCTTGTGTTCAGCTCTTTCCAAATTAAAACAAATATACAT
  TTTCTAAATAAAAATATGTAATTTCAAAATACATTTGTAAGTGTAAAAAATGTCTCATGT
  CAATGACCATTCAATTAGTGGTCATAAAATAGAATAATTCTTTTCTGAGGATAGTAGTTA
  AATAACTGTGTGGCAGTTAATTGGATGTTCACTGCCAGTTGTCTTATGTGAAAAATTAAC
  TTTTTTGTGGCAATTAGTGTGACAGTTTCCAAATTGCCCTATGCTGTGCTCCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000110 unedited
  GAGGATTGTATACGACTCCTATAGGGCGGCCGCGATATTCGCCCTTTGAGGACGCAAGGA
  GGGTTTGTCACTGGCAGACTCGAGACTGTAGGCACTGCCATGGCCCCTGTGCTCAGTAAG
  GACTCGGCGGACATCGAGAGTATCCTGGCTTTAAATCCTCGAACACAAACTCATGCAACT
  CTGTGTTCCACTTCGGCCAAGAAATTAGACAAGAAACATTGGAAAAGAAATCCTGATAAG
  AACTGCTTTAATTGTGAGAAGCTGGAGAATAATTTTGATGACATCAAGCACACGACTCTT
  GGTGAGCGAGGAGCTCTCCGAGAAGCAATGAGATGCCTGAAATGTGCAGATGCCCCGTGT
  CAGAAGAGCTGTCCAACTAATCTTGATATTAAATCATTCATCACAAGTATTGCAAACAAG
  AACTATTATGGAGCTGCTAAGATGATATTTTCTGACAACCCACTTGGTCTGACTTGTGGA
  ATGGTATGTCCAACCTCTGATCTTTGTGTAGGTGGATGCAATTTATATGCCACTGAAGAG
  GGACCCATTAATATTGGTGGATTGCAGCAATTTGCTACTGAGGTATTCAAAGCAATGAGT
  ATCCCACAGATCAGAAATCCTTCGCTGCCTCCCCCAGAAAAAATGTCTGAAGCCTATTCT
  GCAAAGATTGCTCTTTTTGGTGCTGGGCCTGCAAGTATAAGTTGTGCTTCCTTTTTGGCT
  CGATTGGGGTACTCTGACATCACTATATTTGAAAAACAAGAATATGTTGGTGGTTTAAGT
  ACTTCTGAAATTCCTCAGTTCCGCTGCCGTATGATGTAGTGAATTTTGAGATTGAGCTAA
  TGAAGACCTTGGTGTAAAGATAATTTGCGTAAAAGCCTTTCAGTGAATGAAATGACTTCT
  TAGCACTTTGAAAGAAAAAGGCTAACAAAG
  
• Restriction Sites: Please inquire
• ACCN: NM_000110
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000110.3, NP_000101.2
• RefSeq Size: 4451 bp
• RefSeq ORF: 3078 bp
• Locus ID: 1806
• UniProt ID: Q12882
• Cytogenetics: 1p21.3
• Domains: DHOdehase, fer4
• Protein Families: Druggable Genome
• Protein Pathways: beta-Alanine metabolism, Drug metabolism - other enzymes, Metabolic pathways, Pantothenate and CoA biosynthesis, Pyrimidine metabolism
• Gene Summary: The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).