PTCH1 (NM_001083604) Human Untagged Clone

CAT#: SC316080

PTCH1 (untagged)-Human patched 1 (PTCH1), transcript variant 1c

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTCH1
• Synonyms: BCNS; NBCCS; PTC; PTC1; PTCH
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001083604, the custom clone sequence may differ by one or more nucleotides
  ATGTTTAATCCTCAACTCATGATACAGACCCCTAAAGAAGAAGGTGCTAATGTCCTGACC
  ACAGAAGCGCTCCTACAACACCTGGACTCGGCACTCCAGGCCAGCCGTGTCCATGTATAC
  ATGTACAACAGGCAGTGGAAATTGGAACATTTGTGTTACAAATCAGGAGAGCTTATCACA
  GAAACAGGTTACATGGATCAGATAATAGAATATCTTTACCCTTGTTTGATTATTACACCT
  TTGGACTGCTTCTGGGAAGGGGCGAAATTACAGTCTGGGACAGCATACCTCCTAGGTAAA
  CCTCCTTTGCGGTGGACAAACTTCGACCCTTTGGAATTCCTGGAAGAGTTAAAGAAAATA
  AACTATCAAGTGGACAGCTGGGAGGAAATGCTGAATAAGGCTGAGGTTGGTCATGGTTAC
  ATGGACCGCCCCTGCCTCAATCCGGCCGATCCAGACTGCCCCGCCACAGCCCCCAACAAA
  AATTCAACCAAACCTCTTGATATGGCCCTTGTTTTGAATGGTGGATGTCATGGCTTATCC
  AGAAAGTATATGCACTGGCAGGAGGAGTTGATTGTGGGTGGCACAGTCAAGAACAGCACT
  GGAAAACTCGTCAGCGCCCATGCCCTGCAGACCATGTTCCAGTTAATGACTCCCAAGCAA
  ATGTACGAGCACTTCAAGGGGTACGAGTATGTCTCACACATCAACTGGAACGAGGACAAA
  GCGGCAGCCATCCTGGAGGCCTGGCAGAGGACATATGTGGAGGTGGTTCATCAGAGTGTC
  GCACAGAACTCCACTCAAAAGGTGCTTTCCTTCACCACCACGACCCTGGACGACATCCTG
  AAATCCTTCTCTGACGTCAGTGTCATCCGCGTGGCCAGCGGCTACTTACTCATGCTCGCC
  TATGCCTGTCTAACCATGCTGCGCTGGGACTGCTCCAAGTCCCAGGGTGCCGTGGGGCTG
  GCTGGCGTCCTGCTGGTTGCACTGTCAGTGGCTGCAGGACTGGGCCTGTGCTCATTGATC
  GGAATTTCCTTTAACGCTGCAACAACTCAGGTTTTGCCATTTCTCGCTCTTGGTGTTGGT
  GTGGATGATGTTTTTCTTCTGGCCCACGCCTTCAGTGAAACAGGACAGAATAAAAGAATC
  CCTTTTGAGGACAGGACCGGGGAGTGCCTGAAGCGCACAGGAGCCAGCGTGGCCCTCACG
  TCCATCAGCAATGTCACAGCCTTCTTCATGGCCGCGTTAATCCCAATTCCCGCTCTGCGG
  GCGTTCTCCCTCCAGGCAGCGGTAGTAGTGGTGTTCAATTTTGCCATGGTTCTGCTCATT
  TTTCCTGCAATTCTCAGCATGGATTTATATCGACGCGAGGACAGGAGACTGGATATTTTC
  TGCTGTTTTACAAGCCCCTGCGTCAGCAGAGTGATTCAGGTTGAACCTCAGGCCTACACC
  GACACACACGACAATACCCGCTACAGCCCCCCACCTCCCTACAGCAGCCACAGCTTTGCC
  CATGAAACGCAGATTACCATGCAGTCCACTGTCCAGCTCCGCACGGAGTACGACCCCCAC
  ACGCACGTGTACTACACCACCGCTGAGCCGCGCTCCGAGATCTCTGTGCAGCCCGTCACC
  GTGACACAGGACACCCTCAGCTGCCAGAGCCCAGAGAGCACCAGCTCCACAAGGGACCTG
  CTCTCCCAGTTCTCCGACTCCAGCCTCCACTGCCTCGAGCCCCCCTGTACGAAGTGGACA
  CTCTCATCTTTTGCTGAGAAGCACTATGCTCCTTTCCTCTTGAAACCAAAAGCCAAGGTA
  GTGGTGATCTTCCTTTTTCTGGGCTTGCTGGGGGTCAGCCTTTATGGCACCACCCGAGTG
  AGAGACGGGCTGGACCTTACGGACATTGTACCTCGGGAAACCAGAGAATATGACTTTATT
  GCTGCACAATTCAAATACTTTTCTTTCTACAACATGTATATAGTCACCCAGAAAGCAGAC
  TACCCGAATATCCAGCACTTACTTTACGACCTACACAGGAGTTTCAGTAACGTGAAGTAT
  GTCATGTTGGAAGAAAACAAACAGCTTCCCAAAATGTGGCTGCACTACTTCAGAGACTGG
  CTTCAGGGACTTCAGGATGCATTTGACAGTGACTGGGAAACCGGGAAAATCATGCCAAAC
  AATTACAAGAATGGATCAGACGATGGAGTCCTTGCCTACAAACTCCTGGTGCAAACCGGC
  AGCCGCGATAAGCCCATCGACATCAGCCAGTTGACTAAACAGCGTCTGGTGGATGCAGAT
  GGCATCATTAATCCCAGCGCTTTCTACATCTACCTGACGGCTTGGGTCAGCAACGACCCC
  GTCGCGTATGCTGCCTCCCAGGCCAACATCCGGCCACACCGACCAGAATGGGTCCACGAC
  AAAGCCGACTACATGCCTGAAACAAGGCTGAGAATCCCGGCAGCAGAGCCCATCGAGTAT
  GCCCAGTTCCCTTTCTACCTCAACGGCTTGCGGGACACCTCAGACTTTGTGGAGGCAATT
  GAAAAAGTAAGGACCATCTGCAGCAACTATACGAGCCTGGGGCTGTCCAGTTACCCCAAC
  GGCTACCCCTTCCTCTTCTGGGAGCAGTACATCGGCCTCCGCCACTGGCTGCTGCTGTTC
  ATCAGCGTGGTGTTGGCCTGCACATTCCTCGTGTGCGCTGTCTTCCTTCTGAACCCCTGG
  ACGGCCGGGATCATTGTGATGGTCCTGGCGCTGATGACGGTCGAGCTGTTCGGCATGATG
  GGCCTCATCGGAATCAAGCTCAGTGCCGTGCCCGTGGTCATCCTGATCGCTTCTGTTGGC
  ATAGGAGTGGAGTTCACCGTTCACGTTGCTTTGGCCTTTCTGACGGCCATCGGCGACAAG
  AACCGCAGGGCTGTGCTTGCCCTGGAGCACATGTTTGCACCCGTCCTGGATGGCGCCGTG
  TCCACTCTGCTGGGAGTGCTGATGCTGGCGGGATCTGAGTTCGACTTCATTGTCAGGTAT
  TTCTTTGCTGTGCTGGCGATCCTCACCATCCTCGGCGTTCTCAATGGGCTGGTTTTGCTT
  CCCGTGCTTTTGTCTTTCTTTGGACCATATCCTGAGGTGTCTCCAGCCAACGGCTTGAAC
  CGCCTGCCCACACCCTCCCCTGAGCCACCCCCCAGCGTGGTCCGCTTCGCCATGCCGCCC
  GGCCACACGCACAGCGGGTCTGATTCCTCCGACTCGGAGTATAGTTCCCAGACGACAGTG
  TCAGGCCTCAGCGAGGAGCTTCGGCACTACGAGGCCCAGCAGGGCGCGGGAGGCCCTGCC
  CACCAAGTGATCGTGGAAGCCACAGAAAACCCCGTCTTCGCCCACTCCACTGTGGTCCAT
  CCCGAATCCAGGCATCACCCACCCTCGAACCCGAGACAGCAGCCCCACCTGGACTCAGGG
  TCCCTGCCTCCCGGACGGCAAGGCCAGCAGCCCCGCAGGGACCCCCCCAGAGAAGGCTTG
  TGGCCACCCCCCTACAGACCGCGCAGAGACGCTTTTGAAATTTCTACTGAAGGGCATTCT
  GGCCCTAGCAATAGGGCCCGCTGGGGCCCTCGCGGGGCCCGTTCTCACAACCCTCGGAAC
  CCAGCGTCCACTGCCATGGGCAGCTCCGTGCCCGGCTACTGCCAGCCCATCACCACTGTG
  ACGGCTTCTGCCTCCGTGACTGTCGCCGTGCACCCGCCGCCTGTCCCTGGGCCTGGGCGG
  AACCCCCGAGGGGGACTCTGCCCAGGCTACCCTGAGACTGACCACGGCCTGTTTGAGGAC
  CCCCACGTGCCTTTCCACGTCCGGTGTGAGAGGAGGGATTCGAAGGTGGAAGTCATTGAG
  CTGCAGGACGTGGAATGCGAGGAGAGGCCCCGGGGAAGCAGCTCCAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001083604
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083604.1, NP_001077073.1
• RefSeq Size: 7675 bp
• RefSeq ORF: 3891 bp
• Locus ID: 5727
• UniProt ID: Q13635
• Cytogenetics: 9q22.32
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
• Gene Summary: This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (1c) differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences cause translation initiation from a downstream ATG and an isoform (S) with a shorter N-terminus, compared to isoform L. Variants 1c, 1c', 1d, and 1e encode the same isoform (S).