DDX31 (NM_022779) Human Untagged Clone

CAT#: SC314815

DDX31 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX31
• Synonyms: PPP1R25
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022779 edited
  ATTGAGCTCCAGAGCCTGTTAGGGAGCTACCCAGACAGGCTTTAGTTCCTAAAGTGCTGA
  GGAAACGCACGGGGCGGTAAAGGGGAGGCTCGTCGGTGAGATGGCGCCGGATCTCGCTTC
  TCAGAGGCATTCAGAGAGCTTCCCCAGCGTGAACTCTCGGCCGAATGTAATTTTGCCAGG
  CCGCGAGGGACGCAGAGAGGGGCTGCCACCAGGCGGGGGCACGCGCGGTAGTCTTGTCCC
  TACCCGCCCGGTGCCGCCGTCCCCGGCTCCCCTCGGGACGAGTCCGTACAGCTGGAGCCG
  TTCCGGACCCGGAAGGGGCGGTGGCGCAGGGTCCTCGCGCGTCCCACGTGGGGTCCCGGG
  CCCAGCAGTCTGCGCACCGGGCTCGCTGCTGCACCACGCGTCACCCACGCAGACCATGGC
  AGCCGCCGACGGTTCGCTCTTCGACAACCCCAGGACGTTCTCCAGACGTCCCCCAGCCCA
  GGCGAGTCGGCAAGCAAAGGCTACGAAAAGAAAATACCAAGCGTCCAGTGAGGCTCCCCC
  AGCGAAACGGAGGAACGAAACTTCATTTCTCCCAGCCAAGAAAACTAGTGTTAAAGAAAC
  TCAGAGGACTTTTAAGGGGAACGCACAAAAAATGTTTTCTCCAAAGAAGCATTCGGTTAG
  CACAAGTGATAGAAACCAGGAGGAGAGACAGTGCATTAAGACTTCATCACTGTTTAAAAA
  CAACCCTGACATTCCAGAACTCCACAGACCTGTGGTAAAGCAGGTGCAAGAAAAAGTGTT
  TACTTCAGCTGCTTTTCATGAGCTGGGCCTCCACCCACATTTAATTTCCACAATAAATAC
  GGTCTTAAAAATGTCTAGTATGACCAGTGTTCAGAAGCAAAGTATTCCTGTGTTGCTGGA
  AGGCAGAGATGCTCTCGTGAGATCCCAGACGGGCTCAGGTAAAACTCTTGCCTATTGCAT
  CCCTGTGGTCCAGTCCCTTCAAGCAATGGAGTCAAAAATACAGCGCAGTGATGGCCCCTA
  TGCCCTGGTGCTCGTGCCAACGAGAGAGCTAGCTCTACAAAGCTTTGACACTGTCCAGAA
  ACTGCTTAAGCCTTTCACCTGGATTGTGCCTGGAGTGTTAATGGGAGGAGAGAAGAGAAA
  ATCAGAAAAGGCCAGACTCCGCAAAGGAATAAATATCCTTATCTCAACTCCTGGACGCCT
  GGTGGATCATATAAAATCCACAAAGAACATTCATTTTAGTCGGCTGCGGTGGTTGGTGTT
  TGATGAAGCAGACAGAATCTTGGATTTGGGTTTTGAAAAGGACATCACAGTGATACTTAA
  TGCTGTAAATGCTGAATGCCAAAAACGACAGAATGTCTTGCTATCAGCGACACTCACAGA
  AGGTGTAACGCGGCTAGCTGATATCAGTTTGCATGATCCAGTCAGTATTTCTGTCCTGGA
  CAAGAGCCATGACCAGTTGAACCCAAAGGACAAAGCGGTCCAGGAGGTCTGTCCTCCACC
  AGCTGGCGACAAGCTGGACAGCTTTGCAATACCAGAGAGTCTCAAGCAGCATGTGACTGT
  GGTTCCCAGCAAACTGAGGCTTGTCTGCCTAGCGGCCTTCATCCTTCAGAAATGCAAGTT
  TGAGGAAGACCAGAAGATGGTTGTCTTTTTCTCAAGTTGCGAGCTGGTGGAGTTCCACTA
  CAGCCTCTTCCTACAGACCCTGCTGAGCAGCTCAGGGGCGCCGGCATCAGGGCAGTTGCC
  ATCTGCCTCCATGCGATTAAAATTCCTACGGCTGCATGGCGGCATGGAGCAGGAGGAAAG
  AACAGCAGTGTTTCAGGAATTTTCACATTCCAGAAGAGGCGTCCTTCTTTGCACGGATGT
  TGCAGCTCGGGGCTTAGATCTCCCTCAAGTCACGTGGATTGTTCAGTACAACGCTCCATC
  TTCACCTGCAGAATACATCCACCGGATTGGAAGAACCGCCCGGATTGGCTGCCATGGGAG
  CAGCCTGCTCATTTTGGCTCCTTCGGAGGCAGAATATGTCAACTCGTTGGCTTCTCACAA
  AATCAACGTTTCTGAGATTAAGATGGAAGATATTTTGTGTGTTCTGACAAGAGATGATTG
  TTTTAAAGGGAAACGATGGGGAGCCCAGAAATCCCATGCTGTTGGCCCCCAGGAAATCCG
  AGAGCGAGCCACAGTCTTGCAGACGGTATTTGAAGATTACGTGCACTCCAGTGAGAGGAG
  GGTCTCCTGGGCAAAGAAAGCTCTGCAGTCCTTCATCCAAGCCTACGCCACCTACCCCAG
  GGAGCTGAAGCACATCTTCCACGTCCGATCCCTCCACCTTGGGCATGTGGCGAAGAGCTT
  CGGACTAAGAGATGCCCCCAGGAATCTTAGTGCCTTGACTAGAAAGAAGAGGAAAGCACA
  CGTGAAAAGGCCTGACCTTCATAAGAAGACCCAGAGTAAACACAGCCTCGCTGAAATCCT
  ACGTTCGGAATACTCAAGCGGCATGGAGGCCGACGTCGCCAAGGTCAAAAAGCAAAACGC
  ACCTGGAGAGCCTGGTGGCCGGCCCCTGCAGCACAGTCTGCAGCCGACACCCTGCTTTGG
  CCGTGGGAAAACATTAAAATGGAGAAAAACCCAAAAAGGTGTACAGCGGGACAGCAAGAC
  TTCCCAGAAAGTTTAAAATCTCTCTGGGTCTTCGAGTGGAACCTGGAAGCCCCGGGTGGC
  AGTGGATTGATGTCCAGTTCTTGTCAGAGGAGCCTTGTGAAAAATAACTGCGCCTTTGCC
  TGAATACCACGTCCCCAAGAATCAGAGGGGCCACAGCCCTTCTCCTCCATGGGAGGCCCT
  GGAGCCTGATAGAAATCAGTGGTGTGGGGCACTGTAGAGACTAGCCAGCTCCCGACGGAA
  GGGGTCTGTGTCTTGTATTGATCCCATCAGTGCCCGGCACTCCCTTCACCCAGGTGCTGA
  CGTGCCGGGGTTGAGCTGGCACCCCGGGATGTGATGTAAGAACGCCCTGCGCGTCAGCCT
  GGAACGGGCTTGATTGCTGCCCTTAACTGTGTTCTGAAGTTTCACAGCTTGGCTTCAGCC
  TATCAGCGCATACATTGGCATGAATTTTGTGAATTAGGGTGTTAAAAACTGGAATTGAAT
  TTGTACAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022779 unedited
  TCGCATTTTGAATACGACTCACTATAGGGCGGCCGNAATTATTGAGCTCCAGAGCCTGTT
  AGGGAGCTACCCAGACAGGCTTTAGTTCCTAAAGTGCTGAGGAAACGCACGGGGCGGTAA
  AGGGGAGGCTCGTCGGTGAGATGGCGCCGGATCTCGCTTCTCAGAGGCATTCAGAGAGCT
  TCCCCAGCGTGAACTCTCGGCCGAATGTAATTTTGCCAGGCCGCGAGGGACGCAGAGAGG
  GGCTGCCACCAGGCGGGGGCACGCGCGGTAGTCTTGTCCCTACCCGCCCGGTGCCGCCGT
  CCCCGGCTCCCCTCGGGACGAGTCCGTACAGCTGGAGCCGTTCCGGACCCGGAAGGGGCG
  GTGGCGCAGGGTCCTCGCGCGTCCCACGTGGGGTCCCGGGCCCAGCAGTCTGCGCACCGG
  GCTCGCTGCTGCACCACGCGTCACCCACGCAGACCATGGCAGCCGCCGACGGTTCGCTCT
  TCGACAACCCCAGGACGTTCTCCAGACGTCCCCCAGCCCAGGCGAGTCGGCAAGCAAAGG
  CTACGAAAAGAAAATACCAAGCGTCCAGTTGAGCTCCCCCAGCGAAACCGGAGAACGAAA
  CTTCATTTTTCTCCACCCAAGAAAACTAATGGTTAAGGAAACTCAAAAGGACTTTTAGGG
  GGAACGCACAAAAAATGTTTTCTCCAAAGAAGCATTCGGGTAACACAAGTGATGGAAACC
  CGGGGGAAAAGACGGTGCATTAAAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_022779
• Insert Size: 3140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a good match to the protein associated to this reference except for one SNP.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022779.7, NP_073616.6
• RefSeq Size: 3285 bp
• RefSeq ORF: 2556 bp
• Locus ID: 64794
• UniProt ID: Q9H8H2
• Cytogenetics: 9q34.13
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2016]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.