DDX31 Rabbit Polyclonal Antibody

CAT#: TA375388

DDX31 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of A-549 cells, using DDX31 antibody (TA375388) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 3min.

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  Immunofluorescence analysis of HeLa cells using DDX31 antibody (TA375388) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using DDX31 antibody (TA375388) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 652-851 of human DDX31 (NP_073616.6).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 34kDa/64kDa/85kDa/94kDa
• Gene Name: DEAD-box helicase 31
• Database Link:
  Entrez Gene 64794 Human
  UniProt ID Q9H8H2
• Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms.
• Synonyms: FLJ13633; FLJ14578; FLJ23349; helicain