ELMO2 (NM_182764) Human Untagged Clone

CAT#: SC314534

ELMO2 (untagged)-Human engulfment and cell motility 2 (ELMO2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELMO2
• Synonyms: CED-12; Ced-12A; CED12; ELMO-2; VMPI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC314534 representing NM_182764.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCACCACCGTCAGACATTGTCAAAGTGGCCATTGAGTGGCCAGGTGCTAACGCCCAGCTCCTTGAA
  ATCGACCAGAAACGGCCCCTGGCATCCATTATCAAGGAAGTTTGTGATGGGTGGTCGTTGCCAAACCCA
  GAGTATTATACCCTCCGTTATGCAGATGGTCCTCAGCTGTACATCACCGAACAGACTCGCAGTGACATT
  AAGAATGGGACAATCTTACAACTGGCTATCTCCCCGTCCCGGGCTGCACGCCAGCTGATGGAGAGGACC
  CAGTCATCCAACATGGAGACCCGGCTGGATGCCATGAAGGAGCTGGCCAAGCTCTCTGCCGACGTGACT
  TTCGCTACTGAGTTCATCAACATGGATGGCATCATTGTGCTGACAAGGCTCGTGGAAAGTGGAACCAAG
  CTCTTGTCCCACTACAGTGAGATGCTGGCATTCACCCTGACTGCCTTCCTAGAGCTCATGGACCATGGC
  ATTGTCTCCTGGGACATGGTTTCAATCACCTTTATTAAGCAGATTGCAGGGTATGTGAGCCAGCCCATG
  GTGGACGTGTCAATCCTTCAGAGGTCCCTGGCCATCCTGGAGAGCATGGTCTTGAACAGCCAGAGTCTG
  TACCAGAAGATAGCCGAGGAAATCACCGTGGGACAGCTCATCTCACACCTCCAGGTCTCCAACCAGGAG
  ATTCAGACCTACGCCATTGCACTGATTAATGCACTTTTTCTGAAGGCTCCTGAGGACAAACGACAGGAT
  ATGGCAAATGCATTTGCACAGAAGCATCTCCGGTCTATAATCCTGAATCATGTGATCCGAGGGAACCGC
  CCCATCAAAACTGAGATGGCCCATCAGCTATATGTCCTTCAAGTCCTAACCTTTAACCTTCTGGAAGAA
  AGGATGATGACCAAGATGGACCCCAATGACCAGGCTCAAAGGGACATCATATTTGAACTGAGGAGGATT
  GCATTTGACGCAGAGTCTGATCCTAGCAATGCCCCTGGGAGTGGGACCGAAAAACGCAAAGCCATGTAC
  ACAAAGGACTACAAAATGCTGGGATTTACCAACCACATCAATCCAGCCATGGACTTTACCCAGACTCCT
  CCTGGAATGCTGGCCTTGGACAACATGCTGTACTTGGCTAAAGTCCACCAGGACACCTACATCCGGATT
  GTCTTGGAGAACAGTAGCCGGGAAGACAAACATGAATGCCCCTTTGGCCGCAGTGCCATTGAGCTCACC
  AAAATGCTCTGTGAAATCCTGCAGGTTGGGGAACTACCAAATGAAGGACGCAATGACTACCACCCGATG
  TTCTTTACCCATGACCGAGCCTTTGAAGAGCTCTTTGGAATCTGCATCCAGCTGTTGAACAAGACCTGG
  AAGGAGATGAGGGCAACAGCAGAGGACTTCAACAAGGTTATGCAAGTCGTCCGAGAGCAAATCACTCGA
  GCTTTGCCCTCCAAACCCAACTCTTTGGATCAGTTCAAGAGCAAATTGCGTAGCCTGAGTTACTCTGAG
  ATTCTACGACTGCGCCAGTCTGAGAGGATGAGTCAGGATGACTTCCAGTCCCCGCCAATTGTGGAGCTG
  AGGGAGAAGATCCAGCCCGAGATCCTTGAGCTGATCAAGCAGCAGCGCCTGAACCGGCTCTGTGAGGGC
  AGCAGCTTCCGAAAGATTGGGAACCGCCGAAGGCAAGAACGGTTCTGGTACTGCCGGTTGGCACTGAAC
  CACAAGGTCCTTCACTATGGTGACTTGGATGACAACCCACAAGGGGAGGTGACATTTGAATCCCTGCAG
  GAGAAAATTCCTGTTGCAGACATTAAGGCCATTGTCACTGGGAAAGATTGTCCCCACATGAAAGAGAAA
  AGTGCTCTGAAACAGAACAAGGAGGTGTTGGAATTGGCCTTCTCCATCCTGTATGACCCTGATGAGACC
  TTAAACTTCATCGCACCTAATAAATATGAGTACTGCATCTGGATTGATGGCCTCAGTGCCCTTCTGGGG
  AAGGACATGTCCAGTGAGCTGACCAAGAGTGACCTGGACACCCTGCTGAGCATGGAGATGAAGCTGCGG
  CTCCTGGACCTGGAGAACATCCAGATTCCCGAAGCCCCACCCCCCATCCCCAAGGAGCCCAGCAGCTAT
  GACTTTGTCTATCACTATGGCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_182764
• Insert Size: 2163 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182764.2
• RefSeq Size: 4021 bp
• RefSeq ORF: 2163 bp
• Locus ID: 63916
• UniProt ID: Q96JJ3
• Cytogenetics: 20q13.12
• MW: 82.6 kDa
• Gene Summary: The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein. Similarity to a C. elegans protein suggests that this protein may function in phagocytosis of apoptotic cells and in cell migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.