Calpastatin (CAST) (NM_001750) Human Untagged Clone

CAT#: SC314485

CAST (untagged)-Human calpastatin (CAST), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Calpastatin
• Synonyms: BS-17; PLACK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001750 edited
  ATGTCCCAGCCCGGCCAGAAGCCCGCCGCCTCCCCGCGGCCCCGGCGAGCAGCCGCCGCC
  CGCCGCACCCATGAGCATGTCAGTGAAAAAACCAGTGAATCGCCTTCCAAACCAGGAGAA
  AAGAAAGGATCAGATGAGAAAAAAGCAGCAAGCCTCGGCAGCAGTCAATCCTCCAGAACC
  TATGCTGGTGGAACAGCCTCGGCCACCAAGGTGTCAGCTTCCTCTGGTGCAACCAGCAAG
  TCTTCCAGTATGAATCCCACAGAAACCAAGGCCATTCCAGTCAGCCAACAGATGGAAGGA
  CCACATCTTCCTAACAAGAAAAAACACAAAAAACAGGCTGTAAAAACAGAACCTGAGAAG
  AAGTCACAGTCAACCAAGCTGTCTGTGGTTCATGAGAAAAAATCCCAAGAAGGAAAGCCA
  AAAGAACACACAGAGCCAAAAAGCCTACCCAAGCAGGCATCAGATACAGGAAGTAACGAT
  GCTCACAATAAAAAAGCAGTTTCCAGATCAGCTGAACAGCAGCCATCAGAGAAATCAACA
  GAACCAAAGACTAAACCACAAGACATGATTTCTGCTGGTGGAGAGAGTGTTGCTGGTATC
  ACTGCAATATCTGGCAAGCCGGGTGACAAGAAAAAAGAAAAGAAATCATTAACCCCAGCT
  GTGCCAGTTGAATCTAAACCGGATAAACCATCGGGAAAGTCAGGCATGGATGCTGCTTTG
  GATGACTTAATAGATACTTTAGGAGGACCTGAAGAAACTGAAGAAGAAAATACAACGTAT
  ACTGGACCAGAAGTTTCAGATCCAATGAGTTCCACCTACATAGAGGAATTGGGTAAAAGA
  GAAGTCACAATTCCTCCAAAATATAGGGAACTATTGGCTAAAAAGGAAGGGATCACAGGG
  CCTCCTGCAGACTCTTCGAAACCCATAGGGCCAGATGATGCTATAGACGCCTTGTCATCT
  GACTTCACCTGTGGGTCGCCTACAGCTGCTGGAAAGAAAACTGAAAAAGAGGAATCTACA
  GAAGTTTTAAAAGCTCAGTCATCAGGGACAGTCAGAAGTGCTGCTCCACCCCAAGAGAAG
  AAAAGAAAGGTGGAGAAGGATACAATGAGTGATCAAGCACTCGAGGCTCTGTCGGCTTCA
  CTGGGCACCCGGCAAGCAGAACCTGAGCTCGACCTCCGCTCAATTAAGGAAGTCGATGAG
  GCAAAAGCTAAAGAAGAAAAACTAGAGAAGTGTGGTGAGGATGATGAAACAATCCCATCT
  GAGTACAGATTAAAACCAGCCACGGATAAAGATGGAAAACCACTATTGCCAGAGCCTGAA
  GAAAAACCCAAGCCTCGGAGTGAATCAGAACTCATTGATGAACTTTCAGAAGATTTTGAC
  CGGTCTGAATGTAAAGAGAAACCATCTAAGCCAACTGAAAAGACAGAAGAATCTAAGGCC
  GCTGCTCCAGCTCCTGTGTCGGAGGCTGTGTGTCGGACCTCCATGTGTAGTATACAGTCA
  GCACCCCCTGAGCCGGCTACCTTGAAGGGCACAGTGCCAGATGATGCTGTAGAAGCCTTG
  GCTGATAGCCTGGGGAAAAAGGAAGCAGATCCAGAAGATGGAAAACCTGTGATGGATAAA
  GTCAAGGAGAAGGCCAAAGAAGAAGACCGTGAAAAGCTTGGTGAAAAAGAAGAAACAATT
  CCTCCTGATTATAGATTAGAAGAGGTCAAGGATAAAGATGGAAAGCCACTCCTGCCAAAA
  GAGTCTAAGGAACAGCTTCCACCCATGAGTGAAGACTTCCTTCTGGATGCTTTGTCTGAG
  GACTTCTCTGGTCCACAAAATGCTTCATCTCTTAAATTTGAAGATGCTAAACTTGCTGCT
  GCCATCTCTGAAGTGGTTTCCCAAACCCCAGCTTCAACGACCCAAGCTGGAGCCCCACCC
  CGTGATACCTCGCAGAGTGACAAAGACCTCGATGATGCCTTGGATAAACTCTCTGACAGT
  CTAGGACAAAGGCAGCCTGACCCAGATGAGAACAAACCAATGGAAGATAAAGTAAAGGAA
  AAAGCTAAAGCTGAACATAGAGACAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAA
  TACAGACATCTCCTGGATGATAATGGACAGGACAAACCAGTGAAGCCACCTACAAAGAAA
  TCAGAGGATTCAAAGAAACCTGCAGATGACCAAGACCCCATTGATGCTCTCTCAGGAGAT
  CTGGACAGCTGTCCCTCCACTACAGAAACCTCACAGAACACAGCAAAGGATAAGTGCAAG
  AAGGCTGCTTCCAGCTCCAAAGCACCTAAGAATGGAGGTAAAGCGAAGGATTCAGCAAAG
  ACAACAGAGGAAACTTCCAAGCCAAAAGATGACTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001750 unedited
  GGCAACGGAGATTTTGTATACGACTCACTTAGGGCGGCCGCGAATTCGGCACGAGCCTCC
  CTCTCTCCCTGGCAGGACTCCCCGCCAGGCCTCCCCGCCACTCTCCGCGGCGCATTCCGG
  GAGGCAGCGGCCGCAGCGGCCTCGCCATGTCCCAGCCCGGCCAGAAGCCCGCCGCCTCCC
  CGCGGCCCCGGCGAGCAGCCGCCGCCCGCCGCACCCATGAGCATGTCAGTGAAAAAACCA
  GTGAATCGCCTTCCAAACCAGGAGAAAAGAAAGGATCAGATGAGAAAAAAGCAGCAAGCC
  TCGGCAGCAGTCAATCCTCCAGAACCTATGCTGGTGGAACAGCCTCGGCCACCAAGGTGT
  CAGCTTCCTCTGGTGCAACCAGCAAGTCTTCCAGTATGAATCCCACAGAAACCAAGGCCA
  TTCCAGTCAGCCAACAGATGGAAGGACCACATCTTCCTAACAAGAAAAAACACAAAAAAC
  AGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAGTCAACCAAGCTGTCTGTGGTTCATG
  AGAAAAAATCCCAAGAAGGAAAGCCAAAAGAACACACAGAGCCAAAAAGCCTACCCAAGC
  AGGCATCAGATACAGGAAGTAACGATGCTCACAATAAAAAAGCAGTTTCCAGATCAGCTG
  AACAGCAGCCATCAGAGAAATCAACAGAACCAAAGACTAAACCCACAGACATGATNTCTG
  CTGGTGGAGAGAGTGTTGCTGGTATCACTGCAATATCTGGCAA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001750 unedited
  AACAACTCTGATGCCTTGGAAAACTCTCTGACAGTCTAGGACAAAGGCAGCCTGACCCAG
  ATGAGAACAAACCAATGGAAGATAAAGTAAAGGAAAAAGCTAAAGCTGAACATAGAGACA
  AGCTTGGAGAAAGAGATGACACTATCCCACCTGAATACAGACATCTCCTGGATGATAATG
  GACAGGACAAACCAGTGAAGCCACCTACAAAGAAATCAGAGGATTCAAAGAAACCTGCAG
  ATGACCAAGACCCCATTGATGCTCTCTCAGGAGATCTGGACAGCTGTCCCTCCACTACAG
  AAACCTCACAGAACACAGCAAAGGATAAGTGCAAGAAGGCTGCTTCCAGCTCCAAAGCAC
  CTAAGAATGGAGGTAAAGCGAAGGATTCAGCAAAGACAACAGAGGAAACTTCCAAGCCAA
  AAGATGACTAAAGAAATACAAGTTAAGGTATCTGGTATCTGCATGTAAAATCTTCAGCTG
  GTGGATGGTGACTTTTGAAGAACAAAAGGCTTTGGCAACAGAAAACAATTGTTCTGGGTG
  ATTTCTAGAATGGTTTTTGTTGAGTCTCTGAACATCCTAAAATATTGGTTTGTTATTCTT
  TTCCAGAAAGAAAATGAATTTGACTGGTTCACCTGTGTACTGAGTATTGATAAAACTTTG
  AATTTTTTTAATTGCCTTCAATTGGGAGAGAACAGCTTTATATTTGTAAAAAATATATTT
  TGATAAAGTTTCTTAAACGCACCACCAAAAAACAAAAAAAAAAGCCTAAGTGAATTTTTG
  CACATTCTACACACAGTGCCTGGAACACCCACTTGTATTTACGTCCGACCTTAATTTTTT
  TTGTAGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001750
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001750.4, NP_001741.3
• RefSeq Size: 2438 bp
• RefSeq ORF: 2127 bp
• Locus ID: 831
• UniProt ID: P20810
• Cytogenetics: 5q15
• Domains: Calpain_inhib
• Gene Summary: The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.