Apc7 (ANAPC7) (NM_016238) Human Untagged Clone

CAT#: SC314026

ANAPC7 (untagged)-Human anaphase promoting complex subunit 7 (ANAPC7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANAPC7
• Synonyms: APC7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC314026 representing NM_016238.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACCCCGGCGACGCCGCCATTTTGGAGTCTTCCCTAAGGATCCTCTACCGGCTTTTCGAGTCAGTG
  CTGCCGCCGCTGCCCGCGGCTTTGCAGAGCAGGATGAATGTGATAGACCACGTGCGGGACATGGCGGCC
  GCGGGGCTGCACTCCAACGTGCGGCTCCTCAGCAGCTTGTTACTTACAATGAGTAATAACAACCCTGAG
  TTATTCTCCCCACCTCAGAAGTACCAGCTTTTGGTGTATCATGCAGATTCTCTCTTTCATGATAAGGAA
  TATCGGAATGCTGTGAGTAAGTATACCATGGCTTTACAGCAGAAGAAAGCGCTAAGTAAAACTTCAAAA
  GTGAGACCTTCAACTGGAAATTCTGCATCTACTCCACAAAGTCAGTGTCTTCCATCTGAAATTGAAGTG
  AAATACAAAATGGCTGAATGTTATACAATGCTAAAACAAGATAAAGATGCCATTGCTATACTTGATGGG
  ATCCCTTCAAGACAAAGAACTCCCAAAATAAACATGATGCTGGCAAACCTGTACAAGAAGGCTGGTCAG
  GAGCGCCCTTCAGTCACCAGCTATAAGGAGGTGCTGAGGCAGTGCCCATTAGCCCTTGATGCCATTCTA
  GGCTTGTTGTCCCTTTCTGTAAAAGGGGCAGAGGTGGCATCCATGACAATGAATGTGATCCAAACCGTG
  CCTAACTTGGACTGGCTCTCTGTGTGGATCAAAGCGTATGCTTTTGTGCACACTGGTGACAACTCAAGA
  GCAATCAGTACCATCTGTTCACTAGAGAAAAAATCCTTATTGCGAGATAACGTGGACCTATTGGGAAGC
  TTGGCAGATCTGTACTTCAGAGCTGGAGACAATAAAAACTCTGTCCTCAAGTTTGAACAGGCACAGATG
  TTGGATCCTTATCTGATAAAAGGAATGGATGTATATGGCTACCTACTGGCACGAGAAGGGCGGCTAGAG
  GATGTTGAGAACCTTGGATGCCGCCTTTTCAATATCTCTGATCAGCATGCAGAACCGTGGGTGGTTTCT
  GGCTGTCACAGCTTCTATAGCAAACGCTACTCCCGGGCCCTCTATTTAGGAGCCAAGGCCATTCAGCTG
  AACAGTAATAGTGTTCAAGCTCTGCTACTTAAGGGAGCAGCACTTAGGAACATGGGCAGAGTCCAAGAA
  GCAATAATCCACTTTCGGGAGGCCATACGGCTCGCACCTTGTCGCTTAGATTGTTATGAAGGTCTTATC
  GAATGTTACTTAGCCTCCAACAGTATTCGAGAAGCAATGGTAATGGCTAACAACGTTTACAAAACTCTG
  GGAGCAAATGCACAGACCCTTACCCTTTTAGCCACCGTTTGTCTTGAAGACCCAGTGACACAGGAGAAA
  GCCAAAACATTATTAGATAAAGCCCTGACCCAAAGGCCAGATTACATTAAGGCTGTGGTGAAAAAAGCA
  GAACTACTTAGCAGAGAACAGAAATATGAAGATGGAATTGCTTTGCTGAGGAACGCACTGGCTAATCAG
  AGTGACTGTGTCCTGCATCGGATCCTAGGAGATTTCCTTGTAGCTGTCAATGAGTATCAGGAGGCAATG
  GACCAGTATAGTATAGCACTAAGTTTGGACCCCAATGACCAGAAGTCTCTAGAGGGGATGCAGAAGATG
  GAGAAGGAGGAGAGTCCCACGGATGCCACTCAGGAGGAGGATGTGGACGACATGGAAGGGAGTGGGGAA
  GAAGGGGACCTGGAGGGCAGCGACAGTGAGGCGGCCCAGTGGGCTGACCAGGAGCAGTGGTTCGGCATG
  CAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_016238
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016238.2
• RefSeq Size: 3070 bp
• RefSeq ORF: 1800 bp
• Locus ID: 51434
• UniProt ID: Q9UJX3
• Cytogenetics: 12q24.11
• Domains: TPR
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• MW: 66.9 kDa
• Gene Summary: This gene encodes a tetratricopeptide repeat containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for proper protein ubiquitination function of APC/C and for the interaction of APC/C with certain transcription coactivators. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).