DOCK8 (AK093572) Human Untagged Clone

CAT#: SC313702

(untagged)-Human cDNA FLJ36253 fis, clone THYMU2002154

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DOCK8
• Synonyms: HEL-205; MRD2; ZIR8
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK093572, the custom clone sequence may differ by one or more nucleotides
  ATGACACACCTGAACAGCCTGGATGTGCAGCTTGCCCAGGAGCTCGGGGACTTCACTGAT
  GACGACTTGGACGTGGTGTTCACGCCAAAGGAATGTAGGACTTTGCAGCCCTCTTTGCCG
  GAGGAAGGGGTTGAACTGGACCCTCATGTCAGGGACTGTGTTCAGACCTACATCCGTGAG
  TGGCTAATCGTGAACCGGAAAAACCAAGGAAGTCCAGAAATCTGTGGCTTTAAAAAGACT
  GGATCTCGAAAAGATTTTCACAAGACGCTTCCGAAACAGACGTTTGAGTCGGAAACCTTG
  GAGTGCAGTGAACCCGCTGCTCAGGCAGGCCCCCGCCACTTAAACGTGCTGTGCGGCGTG
  TCTGGGAAAGGCCCCGTCACTGCCTGTGACTTTGACCTCCGCAGCCTGCAGCCTGACAAG
  CGGCTAGAAAACCTCCTGCAGCAAGTGAGTGCCGAGGACTTTGAGAAGCAGAACGAGGAG
  GCCCGGAGGACCAACAGGCAGGCCGAGCTCTTTGCCCTTTACCCATCAGTGGACGAGGAG
  GATGCTGTGGAAATACGTCCAGTACCAGAATGTCCCAAGGAACACCTGGGCAACAGAATA
  TTGGTCAAGTTGCTGACCTTGAAGTTCGAGATTGAAATTGAACCCCTGTTTGCCAGCATT
  GCCCTCTACGATGTTAAAGAAAGGAAAAAGATCTCAGAAAATTTTCACTGTGACCTGAAC
  TCTGACCAGTTCAAAGGATTTCTGCGAGCTCACACGCCTTCAGTGGCCGCATCAAGTCAG
  GCGAGATCTGCAGTCTTCTCAGTCACCTACCCGTCCTCAGACATCTACCTGGTAGTCAAG
  ATTGAAAAAGTCCTGCAGCAGGGAGAGATTGGAGACTGTGCAGAGCCCTACACGGTTATC
  AAAGAAAGTGATGGTGGAAAGAGTAAAGAAAAGATTGAAAAACTAAAACTCCAAGCTGAA
  TCCTTCTGCCAGCGTTTGGGGAAATACCGGATGCCCTTTGCCTGGGCACCCATAAGCTTA
  TCAAGCTTCTTCAGTGTCTCCACCCTTGAGAGGGAGGTAACTGATGTGGACTCTGTGGTT
  GGGAGAAGCTCAGTGGGTGAACGGAGGACATTGGCCCAATCTAGAAGGCTTTCTGAAAGA
  GCCCTCTCCTTGGAGGAAAATGGGGTTGGATCCAACTTCAAAACCTCCACTCTGAGCGTT
  AGCAGCTTTTTCAAGCAGGAAGGAGATCGCCTTAGCGATGAAGACTTATTCAAGTTTTTA
  GCTGACTACAAAAGATCATCATCCTTACAGAGACGAGTCAAGTCAATTCCAGGCTTGCTA
  AGACTGGAGATTTCTACAGCTCCAGAGATCATCAATTGCTGTCTGACTCCTGAAATGCTG
  CCCGTGAAACCCTTTCCTGAAAACCGGACACGCCCGCACAAAGAGATTTTGGAATTTCCA
  ACACGAGAAGTATATGTCCCTCACACTGTGTACAGG
  
• Restriction Sites: Please inquire
• ACCN: AK093572
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK093572.1, BAC04199.1
• RefSeq Size: 1873 bp
• RefSeq ORF: 1479 bp
• Locus ID: 81704
• Cytogenetics: 9p24.3
• Gene Summary: This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jun 2010]