GJC1 (NM_005497) Human Untagged Clone

CAT#: SC313291

GJC1 (untagged)-Human gap junction protein, gamma 1, 45kDa (GJC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GJC1
• Synonyms: CX45; GJA7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005497 edited
  GGAGGCGGCGGCGGCGGCGGCAGCGGCGGGAGCGGCTTCTGCCTCGGCTGGAGACTGAGG
  CGAAGGCGGCAGCGGCGGAGGAGGTGGAGGAGAGGCGAGGGTGAAGGCGATGGCGACGCG
  GGGCACATGAGGCCGCGACCGGCGGGACGGGCCGAGGCCCGGCGGAGGAGGCGGCTCCGG
  GGGAACCCGCCGCCGGAGGGAATCTGGAAAAATTGCAATCAAAACATCATTTCTCTAAGA
  AAATTTTGGGTAACCGAAGTTCTGGACAACAGGGCATACCAATTCAATCACCATGAGTTG
  GAGCTTCCTGACTCGCCTGCTAGAGGAGATTCACAACCATTCCACATTTGTGGGGAAGAT
  CTGGCTCACTGTTCTGATTGTCTTCCGGATCGTCCTTACAGCTGTAGGAGGAGAATCCAT
  CTATTACGATGAGCAAAGCAAATTTGTGTGCAACACAGAACAGCCGGGCTGTGAGAATGT
  CTGTTATGATGCGTTTGCACCTCTCTCCCATGTACGCTTCTGGGTGTTCCAGATCATCCT
  GGTGGCAACTCCCTCTGTGATGTACCTGGGCTATGCTATCCACAAGATTGCCAAAATGGA
  GCACGGTGAAGCAGACAAGAAGGCAGCTCGGAGCAAGCCCTATGCAATGCGCTGGAAACA
  ACACCGGGCTCTGGAAGAAACGGAGGAGGACAACGAAGAGGATCCTATGATGTATCCAGA
  GATGGAGTTAGAAAGTGATAAGGAAAATAAAGAGCAGAGCCAACCCAAACCTAAGCATGA
  TGGCCGACGACGGATTCGGGAAGATGGGCTCATGAAAATCTATGTGCTGCAGTTGCTGGC
  AAGGACCGTGTTTGAGGTGGGTTTTCTGATAGGGCAGTATTTTCTGTATGGCTTCCAAGT
  CCACCCGTTTTATGTGTGCAGCAGACTTCCTTGTCCTCATAAGATAGACTGCTTTATTTC
  TAGACCCACTGAAAAGACCATCTTCCTTCTGATAATGTATGGTGTTACAGGCCTTTGCCT
  CTTGCTTAACATTTGGGAGATGCTTCATTTAGGGTTTGGGACCATTCGAGACTCACTAAA
  CAGTAAAAGGAGGGAACTTGAGGATCCGGGTGCTTATAATTATCCTTTCACTTGGAATAC
  ACCATCTGCTCCCCCTGGCTATAACATTGCTGTCAAACCAGATCAAATCCAGTACACCGA
  ACTGTCCAATGCTAAGATCGCCTACAAGCAAAACAAGGCCAACACAGCCCAGGAACAGCA
  GTATGGCAGCCATGAGGAGAACCTCCCAGCTGACCTGGAGGCTCTGCAGCGGGAGATCAG
  GATGGCTCAGGAACGCTTGGATCTGGCAGTTCAGGCCTACAGTCACCAAAACAACCCTCA
  TGGTCCCCGGGAGAAGAAGGCCAAAGTGGGGTCCAAAGCTGGGTCCAACAAAAGCACTGC
  CAGTAGCAAATCAGGGGATGGGAAGACCTCCGTCTGGATTTAATCCTGGCGGGCTTAAAA
  CCTGTGCTTTTCATAGTTTATGGTAAGCAGCAGCTCACTGAATAATGACTTCCATTGAGT
  AAACATTTGGCTCTGGTTATCTTCAGGGATGCTGTTGGCTCATGATCCAAGCTCAGGGGA
  CTCTGGAGGCGGGGCTGGGCTGAGGGAGAGAAAGGGAAACACAGTGTTCCCAGGCACATG
  TTCTTAGCAATAATACAGTTGCAGAACTTTACATTTGTGTCTTCCAGATCTGGAGAAGAA
  CAGACATATTTAAATCATTCTTGTTGAACAGTTTTTGTATGTACAGTATTATGGTACTTT
  TTTTTTTTAGTATGAGAACTTTTTTTGTATTTGTACATTGAACTGCTGTAGTTATACTTT
  TATATTAAAGGGGAAAAAGTCCTTATAAATAATGCCTATTAGGCTAGTGCTATTACTTTG
  TTCAGCAAATTCTACCCTGGCTTTAGAGTTTTTAATAGATGCTACACCTAATAAAAGTGC
  CTCTCATATTGCAGGAACGATTTCAGATGTGTAAGGAGACTGAAGAGGAGATACCAGTTT
  TTAGTCTTGGAAAGCAAGTTCACAGTAGAAGGAGGTTGAGATCTTTCTTTTATGTGAGAA
  ATCTTTGAATCTCATTCATGCGATCAGAGTTGTAGCCAATTTTTGAAAACCTTATTTTCA
  AAGGAAATAAATGATTCACTGTAGGATTCCTTTAAATATCAAGCATCACCAGTATATGCT
  TTGATGGTATATGTATATAACTTAAAGTTCTTTCAAAAGCCTGATACAGAAACGTGTCCC
  CAGTTTGGTAGCAATGTGGAAAACCTGGCTAGAGATGATATGGAGCTGTCCCTCAGAAAG
  CAAAGCCATGCCTGGAATCCCTAATAGGCTGCTTAGTTGTGAACCTGTTTGATTTGCCTT
  AAGCCTCTATCCAGAAACCTGCCCGCTTCCGTCTGGTTAAGAAGCCAGTGGTGGATATTT
  TCTTTGTTAACATTAGAAATGCAAACATTCCCTTGTCAACCAAGAATACTCAAAGCTACT
  TGTATTGGAAATGGCAGAAGGCCTAAATCCAAATTTCTTATTTTTTATAATTTACCATAG
  AAGTTTTGTGATTAAATTCTTACTTCTGCCAGTGGAGGTTTATGCCTGAAAGGTCATGGG
  GTCCTGTCTGTAAATAGACCTAAAGAGAAGTGCAGTATTTATTCTTTGTAGGCATAATGT
  GTTTGTCACTGACAAGCATTCATATTCATCCCACTAGTCTTTTATTGCAGTCTTTTATTG
  TCATTTTCAGCCTTATGTTGGAGAGCTTTGCTTTCTCATCATGTTCACATTGTCTTAAGT
  TTTGTGAGCTTCTGAGAAAGAGCTTGGTAAAGGTTTAAAGGGGACTTTGTTCCACCAGGG
  AGCATTTTATTTGGGCGTCTCACCCTTTTCTAATGAAAGCTGTTGTAAGCCACCTCTGAC
  TTGGAAATTCTGAAAGTATGAATATTTTTTATATCTTAATTGTAAAATGCCAGTTCTCCA
  TTATTTAGATGAATAGTAGAACACTGCACCCTTTGTGCAGTGTTTTTGTTTCTCTACTGC
  ATTCCTACCCCCACCAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005497 unedited
  GGGGTTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCACCAGGGGAGGCGGCGG
  CGGCGGCGGCAGCGGCGGGAGCGGCTTCTGCCTCGGCTGGAGACTGAGGCGAAGGCGGCA
  GCGGCGGAGGAGGTGGAGGAGAGGCGAGGGTGAAGGCGATGGCGACGCGGGGCACATGAG
  GCCGCGACCGGCGGGACGGGCCGAGGCCCGGCGGAGGAGGCGGCTCCGGGGGAACCCGCC
  GCCGGAGGGAATCTGGAAAAATTGCAATCAAAACATCATTTCTCTAAGAAAATTTTGGGT
  AACCGAAGTTCTGGACAACAGGGCATACCAATTCAATCACCATGAGTTGGAGCTTCCTGA
  CTCGCCTGCTAGAGGAGATTCACAACCATTCCACATTTGTGGGGAAGATCTGGCTCACTG
  TTCTGATTGTCTTCCGGATCGTCCTTACAGCTGTAGGAGGAGAATCCATCTATTACGATG
  AGCAAAGCAAATTTGTGTGCAACACAGAACAGCCGGGCTGTGAGAATGTCTGTTATGATG
  CGTTTGCACCTCTCTCCCATGTACGCTTCTGGGTGTTCCAGATCATCCTGGTGGCAACTC
  CCTCTGTGATGTACCTGGGCTATGCTATCCACAAGATTGCCAAAATGGAGCACGGTGAAG
  CAGACAAGAAGGCAGCTCGGAGCAAGCCCTATGCAATGCGCTGGAAACAACACCGGGCTC
  TGGAAGAAACGGAGGAGGACAACGAAGAGGATCCTATGATGTATCCAGAGATGGAGTTAG
  AAAGTGATAAGGAAAATAAAGAGCAGAGCCAACCCAAACCTAAGCATGATGGCCGACGAC
  GGATTCGGGAAGATGGGCTCATGAAAATCTATGTGCTGCAGTTGCTGGCAGGACCGTGTT
  TGAGGTGGGTTTTCTGATAGGGCAGTATTTTCTGTTTGGCTTCCAGTTCCACCCGTTTTA
  TGTTTTGCAGCAGAACTTTCCTTGTCTA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_005497 unedited
  GTACTGTATGGTGTTACAGGCCTTTGCCTCTTGCATTAACATTTGGGAGATGCTTCATTT
  AGGGTTTGGGACCATTCGAGACTCACTAAACAGTAAAAGGAGGGAACTTGAGGATCCGGG
  TGCTTATAATTATCCTTTCACTTGGAATACACCATCTGCTCCCCCTGGCTATAACATTGC
  TGTCAAACCAGATCAAATCCAGTACACCGAACTGTCCAATGCTAAGATCGCCTACAAGCA
  AAACAAGGCCAACACAGCCCAGGAACAGCAGTATGGCAGCCATGAGGAGAACCTCCCAGC
  TGACCTGGAGGCTCTGCAGCGGGAGATCAGGATGGCTCAGGAACGCTTGGATCTGGCAGT
  TCAGGCCTACAGTCACCAAAACAACCCTCATGGTCCCCGGGAGAAGAAGGCCAAAGTGGG
  GTCCAAAGCTGGGTCCAACAAAAGCACTGCCAGTAGCAAATCAGGGGATGGGAAGACCTC
  CGTCTGGATTTAATCCTGGCGGGCTTAAAACCTGTGCTTTTCATAGTTTATGGTAAGCAG
  CAGCTCACTGAATAATGACTTCCATTGAGTAAACATTTGGCTCTGGTTATCTTCAGGGAT
  GCTGTTGGCTCATGATCCAAGCTCAGGGGACTCTGGAGGCGGGGCTGGGCTGAGGGAGAG
  AAAGGGAAACACAGTGTTCCCAGGCACATGTTCTTAGCAATAATACAGTTGCAGAACTTT
  ACATTTGTGTCTTCCAGATCTGGAGAAGAACAGACATATTTAAATCATTCTTGTTGAACA
  GTTTTTGTATGTACAGTATTATGGTACTTTTTTTTTTTAGTATGAGAACTTTTTTTGTAT
  TTGTACATTGACTGCTGTAGTTATACTTTTATATTAAAGGGAAAAGTCCTTATAAATAAT
  GCCTATTAGGCTAGTGCTATTACTTTGTTCAGCAAATTCTACCCTGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_005497
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005497.2, NP_005488.2
• RefSeq Size: 1889 bp
• RefSeq ORF: 1191 bp
• Locus ID: 10052
• UniProt ID: P36383
• Cytogenetics: 17q21.31
• Domains: CNX
• Protein Families: Ion Channels: Other, Transmembrane
• Gene Summary: This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.