BORIS (CTCFL) (NM_080618) Human Untagged Clone

CAT#: SC311151

CTCFL (untagged)-Human CCCTC-binding factor (zinc finger protein)-like (CTCFL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BORIS
• Synonyms: BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_080618 edited
  ATGGCAGCCACTGAGATCTCTGTCCTTTCTGAGCAATTCACCAAGATCAAAGAACTCGAG
  TTGATGCCGGAAAAAGGCCTGAAGGAGGAGGAAAAAGACGGAGTGTGCAGAGAGAAAGAC
  CATCGGAGCCCTAGTGAGTTGGAGGCCGAGCGTACCTCTGGGGCCTTCCAGGACAGCGTC
  CTGGAGGAAGAAGTGGAGCTGGTGCTGGCCCCCTCGGAGGAGAGCGAGAAGTACATCCTG
  ACCCTGCAGACGGTGCACTTCACTTCTGAAGCTGTGGAGTTGCAGGATATGAGCTTGCTG
  AGCATACAGCAGCAAGAAGGGGTGCAGGTGGTGGTGCAACAGCCTGGCCCTGGGTTGCTG
  TGGCTTGAGGAAGGGCCCCGGCAGAGCCTGCAGCAGTGTGTGGCCATTAGTATCCAGCAA
  GAGCTGTACTCCCCGCAAGAGATGGAGGTGTTGCAGTTCCACGCTCTAGAGGAGAATGTG
  ATGGTGGCCAGTGAAGACAGTAAGTTAGCGGTGAGCCTGGCTGAAACTGCTGGACTGATC
  AAGCTCGAGGAAGAGCAGGAGAAGAACCAGTTATTGGCTGAAAGAACAAAGGAGCAGCTC
  TTTTTTGTGGAAACAATGTCAGGAGATGAAAGAAGTGACGAAATTGTTCTCACAGTTTCA
  AATTCAAATGTGGAAGAACAAGAGGATCAACCTACAGCTGGTCAAGCAGATGCTGAAAAG
  GCCAAATCTACAAAAAATCAAAGAAAGACAAAGGGAGCAAAAGGAACCTTCCACTGTGAT
  GTCTGCATGTTCACCTCTTCTAGAATGTCAAGTTTTAATCGTCATATGAAAACTCACACC
  AGTGAGAAGCCTCACCTGTGTCACCTCTGCCTGAAAACCTTCCGTACGGTCACTCTGCTG
  CGGAACCATGTTAACACCCACACAGGAACCAGGCCCTACAAGTGTAACGACTGCAACATG
  GCATTTGTCACCAGTGGAGAACTCGTCCGACACAGGCGCTATAAACATACTCATGAGAAA
  CCCTTTAAATGTTCCATGTGCAAGTATGCCAGTGTGGAGGCAAGTAAATTGAAGCGCCAT
  GTCCGATCCCACACTGGGGAGCGCCCCTTTCAGTGTTGCCAGTGCAGCTATGCCAGCAGA
  GATACCTACAAGCTGAAACGCCACATGAGAACGCACTCAGGTGAGAAGCCTTACGAATGC
  CACATCTGCCACACCCGCTTCACCCAGAGCGGGACCATGAAAATACATATTCTGCAGAAA
  CACGGCGAAAATGTCCCCAAATACCAGTGTCCCCATTGTGCCACCATCATTGCACGGAAA
  AGCGACCTACGTGTGCATATGCGCAACTTGCATGCTTACAGCGCTGCAGAGCTGAAATGC
  CGCTACTGTTCTGCTGTCTTCCATGAACGCTATGCCCTCATTCAGCACCAGAAAACTCAT
  AAGAATGAGAAGAGGTTCAAGTGCAAACACTGCAGTTATGCCTGCAAGCAGGAACGTCAT
  ATGACCGCTCACATTCGTACCCACACTGGAGAGAAACCATTCACCTGCCTTTCTTGCAAT
  AAATGTTTCCGACAGAAGCAACTTCTAAACGCTCACTTCAGGAAATACCACGATGCAAAT
  TTCATCCCGACTGTTTACAAATGCTCCAAGTGTGGCAAAGGCTTTTCCCGCTGGATTAAC
  CTGCACAGACATTCGGAGAAGTGTGGATCAGGGGAAGCAAAGTCGGCTGCTTCAGGAAAG
  GGAAGAAGAACAAGAAAGAGGAAGCAGACCATCCTGAAGGAAGCCACAAAGGGTCAGAAG
  GAAGCTGCGAAGGGATGGAAGGAAGCCGCGAACGGAGACGAAGCTGCTGCTGAGGAGGCT
  TCCACCACGAAGGGAGAACAGTTCCCAGGAGAGATGTTTCCTGTCGCCTGCAGAGAAACC
  ACAGCCAGAGTCAAAGAGGAAGTGGATGAAGGCGTGACCTGTGAAATGCTCCTCAACACG
  ATGGATAAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080618 unedited
  NNGAGGTCAATATTGTATACCACTCATATAGGCGGCCGCAATCTAGAGTCGAGCCGGCGG
  CGGCCGGCTGTGGGCTGCAGCACGCGGTGCACGAGGCAGAGCCCACAAGCCAAAGACGGA
  GTGGGCCGAGCATTCCGGCCACGCCTTCCGCGGCCAAGTCATTATGGCAGCCACTGAGAT
  CTCTGTCCTTTCTGAGCAATTCACCAAGATCAAAGAACTCGAGTTGATGCCGGAAAAAGG
  CCTGAAGGAGGAGGAAAAAGACGGAGTGTGCAGAGAGAAAGACCATCGGAGCCCTAGTGA
  GTTGGAGGCCGAGCGTACCTCTGGGGCCTTCCAGGACAGCGTCCTGGAGGAAGAAGTGGA
  GCTGGTGCTGGCCCCCTCGGAGGAGAGCGAGAAGTACATCCTGACCCTGCAGACGGTGCA
  CTTCACTTCTGAAGCTGTGGAGTTGCAGGATATGAGCTTGCTGAGCATACAGCAGCAAGA
  AGGGGTGCAGGTGGTGGTGCAACAGCCTGGCCCTGGGTTGCTGTGGCTTGAGGAAGGGCC
  CCGGCAGAGCCTGCAGCAGTGTGTGGCCATTAGTATCCAGCAAGAGCTGTACTCCCCGCA
  AGAGATGGAGGTGTTGCAGTTCCACGCTCTAGAGGAGAATGTGATGGTGGCCAGTGAAGA
  CAGTAAGTTAGCGGTGAGCCTGGCTGAAACTGCTGGACTGATCAAGCTCGAGGAAGAGCA
  NGAGAAGAACCAGTTATTGGCTGANAGAACANAGGAGCAGCTCTTTTTTGTGGAAACATG
  TCAGGAGATGAAAGAAGTGACGAAATTGTCTCACAGTTTCAAATTCAATGGTGGAGAACA
  AGNANATCAACCTACAGCTGGTCAAGCAGATGCTGAAAAGGCC
  
• Restriction Sites: Please inquire
• ACCN: NM_080618
• Insert Size: 3600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080618.2, NP_542185.2
• RefSeq Size: 3493 bp
• RefSeq ORF: 1992 bp
• Locus ID: 140690
• UniProt ID: Q8NI51
• Cytogenetics: 20q13.31
• Protein Families: Transcription Factors
• Gene Summary: CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
  Transcript Variant: This variant (2, also know as A2) has an additional exon in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same isoform (1).