BORIS(CTCFL) (NM_080618) Human Tagged ORF Clone

CAT#: RC216042

CTCFL (Myc-DDK-tagged)-Human CCCTC-binding factor (zinc finger protein)-like (CTCFL)

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Size
    • 10 ug

Cited in 2 publications.

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Specifications

Product Data
Product Name BORIS(CTCFL) (NM_080618) Human Tagged ORF Clone
Symbol CTCFL
Synonyms BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
Vector pCMV6-Entry
Sequence Data
>RC216042 representing NM_080618
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCACTGAGATCTCTGTCCTTTCTGAGCAATTCACCAAGATCAAAGAACTCGAGTTGATGCCGG
AAAAAGGCCTGAAGGAGGAGGAAAAAGACGGAGTGTGCAGAGAGAAAGACCATCGGAGCCCTAGTGAGTT
GGAGGCCGAGCGTACCTCTGGGGCCTTCCAGGACAGCGTCCTGGAGGAAGAAGTGGAGCTGGTGCTGGCC
CCCTCGGAGGAGAGCGAGAAGTACATCCTGACCCTGCAGACGGTGCACTTCACTTCTGAAGCTGTGGAGT
TGCAGGATATGAGCTTGCTGAGCATACAGCAGCAAGAAGGGGTGCAGGTGGTGGTGCAACAGCCTGGCCC
TGGGTTGCTGTGGCTTGAGGAAGGGCCCCGGCAGAGCCTGCAGCAGTGTGTGGCCATTAGTATCCAGCAA
GAGCTGTACTCCCCGCAAGAGATGGAGGTGTTGCAGTTCCACGCTCTAGAGGAGAATGTGATGGTGGCCA
GTGAAGACAGTAAGTTAGCGGTGAGCCTGGCTGAAACTGCTGGACTGATCAAGCTCGAGGAAGAGCAGGA
GAAGAACCAGTTATTGGCTGAAAGAACAAAGGAGCAGCTCTTTTTTGTGGAAACAATGTCAGGAGATGAA
AGAAGTGACGAAATTGTTCTCACAGTTTCAAATTCAAATGTGGAAGAACAAGAGGATCAACCTACAGCTG
GTCAAGCAGATGCTGAAAAGGCCAAATCTACAAAAAATCAAAGAAAGACAAAGGGAGCAAAAGGAACCTT
CCACTGTGATGTCTGCATGTTCACCTCTTCTAGAATGTCAAGTTTTAATCGTCATATGAAAACTCACACC
AGTGAGAAGCCTCACCTGTGTCACCTCTGCCTGAAAACCTTCCGTACGGTCACTCTGCTGCGGAACCATG
TTAACACCCACACAGGAACCAGGCCCTACAAGTGTAACGACTGCAACATGGCATTTGTCACCAGTGGAGA
ACTCGTCCGACACAGGCGCTATAAACATACTCATGAGAAACCCTTTAAATGTTCCATGTGCAAGTATGCC
AGTGTGGAGGCAAGTAAATTGAAGCGCCATGTCCGATCCCACACTGGGGAGCGCCCCTTTCAGTGTTGCC
AGTGCAGCTATGCCAGCAGAGATACCTACAAGCTGAAACGCCACATGAGAACGCACTCAGGTGAGAAGCC
TTACGAATGCCACATCTGCCACACCCGCTTCACCCAGAGCGGGACCATGAAAATACATATTCTGCAGAAA
CACGGCGAAAATGTCCCCAAATACCAGTGTCCCCATTGTGCCACCATCATTGCACGGAAAAGCGACCTAC
GTGTGCATATGCGCAACTTGCATGCTTACAGCGCTGCAGAGCTGAAATGCCGCTACTGTTCTGCTGTCTT
CCATGAACGCTATGCCCTCATTCAGCACCAGAAAACTCATAAGAATGAGAAGAGGTTCAAGTGCAAACAC
TGCAGTTATGCCTGCAAGCAGGAACGTCATATGACCGCTCACATTCGTACCCACACTGGAGAGAAACCAT
TCACCTGCCTTTCTTGCAATAAATGTTTCCGACAGAAGCAACTTCTAAACGCTCACTTCAGGAAATACCA
CGATGCAAATTTCATCCCGACTGTTTACAAATGCTCCAAGTGTGGCAAAGGCTTTTCCCGCTGGATTAAC
CTGCACAGACATTCGGAGAAGTGTGGATCAGGGGAAGCAAAGTCGGCTGCTTCAGGAAAGGGAAGAAGAA
CAAGAAAGAGGAAGCAGACCATCCTGAAGGAAGCCACAAAGGGTCAGAAGGAAGCTGCGAAGGGATGGAA
GGAAGCCGCGAACGGAGACGAAGCTGCTGCTGAGGAGGCTTCCACCACGAAGGGAGAACAGTTCCCAGGA
GAGATGTTTCCTGTCGCCTGCAGAGAAACCACAGCCAGAGTCAAAGAGGAAGTGGATGAAGGCGTGACCT
GTGAAATGCTCCTCAACACGATGGATAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC216042 representing NM_080618
Red=Cloning site Green=Tags(s)

MAATEISVLSEQFTKIKELELMPEKGLKEEEKDGVCREKDHRSPSELEAERTSGAFQDSVLEEEVELVLA
PSEESEKYILTLQTVHFTSEAVELQDMSLLSIQQQEGVQVVVQQPGPGLLWLEEGPRQSLQQCVAISIQQ
ELYSPQEMEVLQFHALEENVMVASEDSKLAVSLAETAGLIKLEEEQEKNQLLAERTKEQLFFVETMSGDE
RSDEIVLTVSNSNVEEQEDQPTAGQADAEKAKSTKNQRKTKGAKGTFHCDVCMFTSSRMSSFNRHMKTHT
SEKPHLCHLCLKTFRTVTLLRNHVNTHTGTRPYKCNDCNMAFVTSGELVRHRRYKHTHEKPFKCSMCKYA
SVEASKLKRHVRSHTGERPFQCCQCSYASRDTYKLKRHMRTHSGEKPYECHICHTRFTQSGTMKIHILQK
HGENVPKYQCPHCATIIARKSDLRVHMRNLHAYSAAELKCRYCSAVFHERYALIQHQKTHKNEKRFKCKH
CSYACKQERHMTAHIRTHTGEKPFTCLSCNKCFRQKQLLNAHFRKYHDANFIPTVYKCSKCGKGFSRWIN
LHRHSEKCGSGEAKSAASGKGRRTRKRKQTILKEATKGQKEAAKGWKEAANGDEAAAEEASTTKGEQFPG
EMFPVACRETTARVKEEVDEGVTCEMLLNTMDK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_080618
ORF Size 1989 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_080618.1, NM_080618.2, NM_080618.3, NP_542185
RefSeq Size 3493
RefSeq ORF 1992
Locus ID 140690
Cytogenetics 20q13.31
Protein Families Transcription Factors
MW 75.6 kDa
Gene Summary CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012].
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Citations (2)