ENTR1 (NM_001039707) Human Untagged Clone

CAT#: SC310811

SDCCAG3 (untagged)-Human serologically defined colon cancer antigen 3 (SDCCAG3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ENTR1
• Synonyms: NY-CO-3; SDCCAG3; SDDAG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310811 representing NM_001039707.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCGGGCTACCAGCGCCGCCCGGGCGCCACCCCGCTGTCCCGAGCCCGGAGCCTCGCCATTCCCGAC
  GCTCCAGCGTTCTATGAGCGCCGGTCTTGTCTCCCCCAGCTAAATTGTGAGCGCCCCCATGGCAGGGAC
  CTGGACTCCCCCTTCTTCGGCATTCGGCCGGCCTTTATGTGCTATGTGCCCAGCCCGGTGCTGGCTTCC
  GTGGGAGACACAGATTTTGGCTATGGAAAGGGGAAATGTTCTAAGCAGAGCCCGTCAGGAGCCCACGGG
  ACACATTTTGGAGATGACAGATTTGAAGATCTGGAAGAGGCAAATCCATTCTCTTTTAGAGAGTTTCTG
  AAGACCAAGAACCTCGGCCTCTCGAAAGAGGATCCGGCCAGCAGAATTTATGCAAAGGAAGCCTCGAGG
  CATTCCCTGGGACTTGACCACAACTCCCCACCCTCCCAAACCGGCGGGTATGGCCTGGAGTATCAGCAG
  CCATTTTTCGAGGATCCGACAGGGGCTGGTGACCTCCTGGATGAGGAGGAGGATGAGGACACCGGATGG
  AGTGGGGCCTACCTGCCGTCCGCCATCGAGCAGACTCACCCCGAGAGGGTCCCTGCCGGCACGTCGCCC
  TGCAGCACATACCTTTCCTTTTTCTCCACCCCGTCGGAGCTGGCAGGGCCTGAGTCTCTGCCCTCGTGG
  GCGTTGAGTGACACTGATTCTCGCGTGTCTCCGGCCTCTCCGGCAGGGAGTCCTAGCGCAGACTTTGCG
  GTTCATGGAGAGTCTCTGGGAGACAGGCACCTGCGGACGCTGCAGATAAGTTACGACGCACTGAAAGAT
  GAAAATTCTAAGCTGAGAAGAAAGCTGAATGAGGTTCAGAGCTTCTCTGAAGCTCAAACAGAAATGGTG
  AGGACGCTTGAGCGGAAGTTAGAAGCAAAAATGATCAAGGAGGAAAGCGACTACCACGACCTGGAGTCG
  GTGGTTCAGCAGGTGGAGCAGAACCTGGAGCTGATGACCAAACGGGCTGTAAAGGCAGAAAACCACGTC
  GTGAAACTAAAACAGGAAATCAGTTTGCTCCAGGCGCAGGTCTCCAACTTCCAGCGAGAGAATGAAGCC
  CTGCGGTGCGGCCAGGGTGCCAGCCTGACCGTGGTGAAGCAGAACGCCGACGTGGCCCTGCAGAACCTC
  CGGGTGGTCATGAACAGTGCACAGGCTTCCATCAAGCAACTGGTTTCCGGAGCTGAGACACTGAATCTT
  GTTGCCGAAATCCTTAAATCTATAGACAGAATTTCTGAAGTTAAAGACGAGGAGGAAGACTCTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001039707
• Insert Size: 1308 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039707.1
• RefSeq Size: 2390 bp
• RefSeq ORF: 1308 bp
• Locus ID: 10807
• UniProt ID: Q96C92
• Cytogenetics: 9q34.3
• MW: 48 kDa
• Gene Summary: Endosome-associated protein that plays a role in membrane receptor sorting, cytokinesis and ciliogenesis (PubMed:23108400, PubMed:25278552, PubMed:27767179). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1 (PubMed:25278552). Involved in the regulation of cytokinesis; the function may involve PTPN13 and GIT1 (PubMed:23108400). Plays a role in the formation of cilia (PubMed:27767179). Involved in cargo protein localization, such as PKD2, at primary cilia (PubMed:27767179). Involved in the presentation of the tumor necrosis factor (TNF) receptor TNFRSF1A on the cell surface, and hence in the modulation of the TNF-induced apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).