PRDM15 (NM_001040424) Human Untagged Clone

CAT#: SC310722

PRDM15 (untagged)-Human PR domain containing 15 (PRDM15), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRDM15
• Synonyms: C21orf83; PFM15; ZNF298
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001040424, the custom clone sequence may differ by one or more nucleotides
  ATGCCCCGCCGCCGCCCCCCGGCTTCCGGTGCTGCGCAGTTTCCGGAGCGGATCGCAACC
  CGGAGTCCGGATCCGATCCCGCTCTGCACATTCCAAAGGCAGGTGTCAGAGATGGCTGAA
  GATGGGAGCGAAGAGATCATGTTCATCTGGTGTGAAGACTGCAGCCAGTACCACGACTCC
  GAATGTCCCGAGCTGGGCCCAGTGGTCATGGTCAAAGACTCCTTTGTGTTAAGCAGGGCA
  AGGTCATCCCTTCCTCCCAACTTGGAGATCAGACGACTGGAAGATGGAGCCGAGGGGGTG
  TTCGCCATCACTCAGCTCGTCAAGCGGACACAGTTCGGTCCCTTTGAGTCCAGGAGGGTC
  GCCAAATGGGAAAAGGAGTCTGCATTTCCCCTGAAGGTGTTCCAGAAGGACGGGCACCCC
  GTGTGCTTCGACACCTCCAACGAGGATGACTGCAACTGGATGATGCTGGTGCGGCCAGCG
  GCGGAGGCCGAGCACCAGAACCTGACGGCCTACCAGCACGGCAGCGACGTGTACTTCACC
  ACCTCCAGAGACATCCCCCCGGGTACCGAGCTGCGCGTGTGGTATGCGGCCTTCTATGCC
  AAGAAGATGGACAAGCCCATGCTGAAGCAGGCCGGCTCTGGCGTCCACGCTGCAGGCACC
  CCAGAAAACAGCGCCCCCGTGGAGTCGGAGCCCAGCCAGTGGGCGTGTAAAGTGTGTTCT
  GCCACCTTCCTGGAGCTGCAGCTCCTCAATGAACATCTGTTGGGCCACTTAGAACAAGCC
  AAAAGCCTTCCTCCAGGCAGCCAAAGCGAGGCAGCAGCTCCCGAGAAGGAGCAGGACACA
  CCCCGGGGGGAACCCCCTGCAGTGCCCGAGAGCGAGAATGTTGCCACCAAAGAACAGAAG
  AAAAAGCCTCGAAGGGGGAGAAAACCCAAAGTGTCCAAAGCTGAGCAGCCTCTAGTCATC
  GTGGAAGACAAGGAACCCACAGAGCAAGTGGCAGAGATCATTACCGAGGTCCCTCCGGAT
  GAGCCTGTGAGTGCAACGCCAGATGAGCGGATCATGGAGCTGGTTCTGGGGAAGCTGGCC
  ACCACCACCACTGACACCAGCTCGGTTCCAAAGTTCACCCATCATCAGAATAACACCATC
  ACGCTCAAGAGGAGCTTAATTCTCTCAAGCAGACACGGCATCCGGCGCAAGCTCATCAAA
  CAGCTCGGGGAGCACAAGCGGGTTTACCAGTGCAATATCTGCAGCAAGATCTTCCAGAAC
  AGCAGCAACCTGAGCAGGCACGTGCGCTCGCATGGTGACAAGCTGTTTAAGTGCGAAGAG
  TGTGCAAAATTGTTCAGCCGCAAAGAGAGCCTAAAGCAGCACGTTTCCTACAAGCACAGC
  AGGAACGAGGTGGACGGCGAGTACAGGTACCGCTGCGGCACTTGTGAGAAGACCTTCCGC
  ATCGAGAGCGCGCTGGAGTTCCACAACTGCAGGACAGATGACAAGACGTTCCAATGTGAG
  ATGTGTTTCAGATTCTTCTCCACCAACAGCAACCTCTCCAAGCACAAGAAGAAGCACGGC
  GACAAGAAGTTTGCCTGTGAGGTCTGCAGCAAGATGTTCTACCGCAAGGACGTCATGCTG
  GACCACCAGCGCCGGCACCTGGAAGGAGTGCGGCGAGTGAAGCGAGAGGACCTGGAGGCC
  GGTGGGGAGAACCTGGTCCGTTACAAGAAGGAGCCTTCCGGGTGCCCGGTGTGTGGCAAG
  GTGTTCTCCTGCCGGAGCAATATGAACAAGCACCTGCTCACCCACGGCGACAAGAAGTAC
  ACCTGCGAGATCTGCGGGCGCAAGTTCTTCCGCGTGGATGTGCTCAGGGACCACATCCAT
  GTCCACTTCAAGGACATCGCGTTGATGGATGACCACCAGAGGGAAGAGTTTATCGGCAAG
  ATCGGGATCTCCTCGGAAGAAAACGATGACAATTCTGACGAGAGCGCAGACTCGGAGCCT
  CACAAGTACAGCTGCAAGCGGTGCCAGCTCACCTTCGGCCGGGGGAAGGAGTACCTGAAG
  CACATCATGGAGGTGCACAAGGAGAAGGGCTATGGCTGCAGCATCTGCAACCGGCGCTTT
  GCACTGAAGGCCACCTACCACGCCCACATGGTCATCCACCGTGAAAACCTGCCGGACCCC
  AACGTGCAGAAGTACATCCACCCCTGCGAGATCTGCGGGCGGATCTTCAACAGCATCGGG
  AACCTGGAGCGCCACAAGCTCATCCACACAGGTGTGAAGAGCCACGCCTGCGAGCAGTGT
  GGGAAGTCCTTTGCCAGGAAGGACATGCTGAAGGAGCACATGCGTGTGCACGACAATGTC
  CGCGAGTACCTGTGTGCCGAGTGTGGGAAAGGCATGAAGACCAAGCACGCGCTGCGCCAC
  CACATGAAGCTGCACAAGGGCATCAAGGAGTACGAGTGCAAGGAGTGCCACCGCAGGTTC
  GCGCAGAAGGTCAACATGCTCAAGCACTGCAAGCGGCACACGGGGATTAAAGATTTCATG
  TGTGAATTGTGTGGGAAGACATTCAGCGAGAGGAACACCATGGAGACCCACAAGCTCATC
  CACACAGTGGGCAAGCAGTGGACGTGCTCCGTGTGCGACAAGAAGTACGTGACCGAGTAC
  ATGCTGCAGAAGCACGTTCAGCTCACACACGACAAGGTGGAGGCGCAGAGCTGCCAGCTG
  TGCGGGACCAAGGTGTCCACCAGGGCCTCCATGAGCCGACACATGCGGCGCAAGCACCCC
  GAGGTGCTCGCGGTGAGGATCGATGACCTGGACCACCTCCCGGAGACCACCACCATCGAC
  GCCTCCTCCATTGGCATCGTCCAGCCTGAGCTGACTCTGGAGCAGGAGGATTTGGCCGAA
  GGGAAGCACGGGAAAGCTGCCAAGCGAAGTCACAAGAGAAAGCAGAAGCCAGAAGAGGAG
  GCGGGTGCTCCGGTGCCCGAGGACGCCACCTTCAGCGAATACTCAGAGAAAGAGACGGAG
  TTCACAGGCAGTGTAGGCGACGAGACCAATTCCGCAGTACAGAGCATTCAGCAGGTAGTG
  GTGACCCTGGGTGACCCAAATGTGACCACACCATCGAGCTCAGTCGGCTTAACCAACATC
  ACCGTGACCCCCATCACCACTGCGGCCGCGACTCAGTTTACCAATCTCCAGCCGGTGGCC
  GTGGGGCACCTTACCACCCCTGAACGCCAGTTACAGCTGGACAACTCAATCCTGACCGTG
  ACCTTTGATACCGTCAGCGGCTCTGCCATGTTGCACAACCGCCAAAATGACGTCCAGATC
  CACCCCCAGCCGGAAGCCTCGAACCCACAGTCTGTGGCCCATTTCATCAACCTGACGACC
  CTGGTCAACTCCATCACGCCCCTGGGGAGCCAGCTTAGTGACCAGCACCCGCTCACGTGG
  CGGGCAGTGCCCCAGACTGACGTCTTGCCACCCTCGCAGCCGCAGGCACCCCCACAGCAG
  GCGGCCCAGCCCCAGGTGCAGGCGGAGCAGCAGCAGCAGCAGATGTACAGCTACTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001040424
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040424.1, NP_001035514.1
• RefSeq Size: 6654 bp
• RefSeq ORF: 3537 bp
• Locus ID: 63977
• UniProt ID: P57071
• Cytogenetics: 21q22.3
• Gene Summary: Sequence-specific DNA-binding transcriptional regulator. Plays a role as a molecular node in a transcriptional network regulating embryonic development and cell fate decision. Stimulates the expression of upstream key transcriptional activators and repressors of the Wnt/beta-catenin and MAPK/ERK pathways, respectively, that are essential for naive pluripotency and self-renewal maintenance of embryonic stem cells (ESCs). Specifically promotes SPRY1 and RSPO1 transcription activation through recognition and direct binding of a specific DNA sequence in their promoter regions. Involved in early embryo development (By similarity). Plays also a role in induced pluripotent stem cells (iPSCs) reprogramming (PubMed:28740264).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks seven alternate in-frame exons and differs in the 3' UTR, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.