PRDM15 (NM_001040424) Human Tagged ORF Clone

CAT#: RG219769

PRDM15 (tGFP-tagged) - Human PR domain containing 15 (PRDM15), transcript variant 2

Product Images

Circular map for RG219769

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PRDM15
• Synonyms: C21orf83; PFM15; ZNF298
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219769 representing NM_001040424
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGCCGCCGCCCCCCGGCTTCCGGTGCTGCGCAGTTTCCGGAGCGGATCGCAACCCGGAGTCCGG
  ATCCGATCCCGCTCTGCACATTCCAAAGGCAGGTGTCAGAGATGGCTGAAGATGGGAGCGAAGAGATCAT
  GTTCATCTGGTGTGAAGACTGCAGCCAGTACCACGACTCCGAATGTCCCGAGCTGGGCCCAGTGGTCATG
  GTCAAAGACTCCTTTGTGTTAAGCAGGGCAAGGTCATCCCTTCCTCCCAACTTGGAGATCAGACGACTGG
  AAGATGGAGCCGAGGGGGTGTTCGCCATCACTCAGCTCGTCAAGCGGACACAGTTCGGTCCCTTTGAGTC
  CAGGAGGGTCGCCAAATGGGAAAAGGAGTCTGCATTTCCCCTGAAGGTGTTCCAGAAGGACGGGCACCCC
  GTGTGCTTCGACACCTCCAACGAGGATGACTGCAACTGGATGATGCTGGTGCGGCCAGCGGCGGAGGCCG
  AGCACCAGAACCTGACGGCCTACCAGCACGGCAGCGACGTGTACTTCACCACCTCCAGAGACATCCCCCC
  GGGTACCGAGCTGCGCGTGTGGTATGCGGCCTTCTATGCCAAGAAGATGGACAAGCCCATGCTGAAGCAG
  GCCGGCTCTGGCGTCCACGCTGCAGGCACCCCAGAAAACAGCGCCCCCGTGGAGTCGGAGCCCAGCCAGT
  GGGCGTGTAAAGTGTGTTCTGCCACCTTCCTGGAGCTGCAGCTCCTCAATGAACATCTGTTGGGCCACTT
  AGAACAAGCCAAAAGCCTTCCTCCAGGCAGCCAAAGCGAGGCAGCAGCTCCCGAGAAGGAGCAGGACACA
  CCCCGGGGGGAACCCCCTGCAGTGCCCGAGAGCGAGAATGTTGCCACCAAAGAACAGAAGAAAAAGCCTC
  GAAGGGGGAGAAAACCCAAAGTGTCCAAAGCTGAGCAGCCTCTAGTCATCGTGGAAGACAAGGAACCCAC
  AGAGCAAGTGGCAGAGATCATTACCGAGGTCCCTCCGGATGAGCCTGTGAGTGCAACGCCAGATGAGCGG
  ATCATGGAGCTGGTTCTGGGGAAGCTGGCCACCACCACCACTGACACCAGCTCGGTTCCAAAGTTCACCC
  ATCATCAGAATAACACCATCACGCTCAAGAGGAGCTTAATTCTCTCAAGCAGACACGGCATCCGGCGCAA
  GCTCATCAAACAGCTCGGGGAGCACAAGCGGGTTTACCAGTGCAATATCTGCAGCAAGATCTTCCAGAAC
  AGCAGCAACCTGAGCAGGCACGTGCGCTCGCATGGTGACAAGCTGTTTAAGTGCGAAGAGTGTGCAAAAT
  TGTTCAGCCGCAAAGAGAGCCTAAAGCAGCACGTTTCCTACAAGCACAGCAGGAACGAGGTGGACGGCGA
  GTACAGGTACCGCTGCGGCACTTGTGAGAAGACCTTCCGCATCGAGAGCGCGCTGGAGTTCCACAACTGC
  AGGACAGATGACAAGACGTTCCAATGTGAGATGTGTTTCAGATTCTTCTCCACCAACAGCAACCTCTCCA
  AGCACAAGAAGAAGCACGGCGACAAGAAGTTTGCCTGTGAGGTCTGCAGCAAGATGTTCTACCGCAAGGA
  CGTCATGCTGGACCACCAGCGCCGGCACCTGGAAGGAGTGCGGCGAGTGAAGCGAGAGGACCTGGAGGCC
  GGTGGGGAGAACCTGGTCCGTTACAAGAAGGAGCCTTCCGGGTGCCCGGTGTGTGGCAAGGTGTTCTCCT
  GCCGGAGCAATATGAACAAGCACCTGCTCACCCACGGCGACAAGAAGTACACCTGCGAGATCTGCGGGCG
  CAAGTTCTTCCGCGTGGATGTGCTCAGGGACCACATCCATGTCCACTTCAAGGACATCGCGTTGATGGAT
  GACCACCAGAGGGAAGAGTTTATCGGCAAGATCGGGATCTCCTCGGAAGAAAACGATGACAATTCTGACG
  AGAGCGCAGACTCGGAGCCTCACAAGTACAGCTGCAAGCGGTGCCAGCTCACCTTCGGCCGGGGGAAGGA
  GTACCTGAAGCACATCATGGAGGTGCACAAGGAGAAGGGCTATGGCTGCAGCATCTGCAACCGGCGCTTT
  GCACTGAAGGCCACCTACCACGCCCACATGGTCATCCACCGTGAAAACCTGCCGGACCCCAACGTGCAGA
  AGTACATCCACCCCTGCGAGATCTGCGGGCGGATCTTCAACAGCATCGGGAACCTGGAGCGCCACAAGCT
  CATCCACACAGGTGTGAAGAGCCACGCCTGCGAGCAGTGTGGGAAGTCCTTTGCCAGGAAGGACATGCTG
  AAGGAGCACATGCGTGTGCACGACAATGTCCGCGAGTACCTGTGTGCCGAGTGTGGGAAAGGCATGAAGA
  CCAAGCACGCGCTGCGCCACCACATGAAGCTGCACAAGGGCATCAAGGAGTACGAGTGCAAGGAGTGCCA
  CCGCAGGTTCGCGCAGAAGGTCAACATGCTCAAGCACTGCAAGCGGCACACGGGGATTAAAGATTTCATG
  TGTGAATTGTGTGGGAAGACATTCAGCGAGAGGAACACCATGGAGACCCACAAGCTCATCCACACAGTGG
  GCAAGCAGTGGACGTGCTCCGTGTGCGACAAGAAGTACGTGACCGAGTACATGCTGCAGAAGCACGTTCA
  GCTCACACACGACAAGGTGGAGGCGCAGAGCTGCCAGCTGTGCGGGACCAAGGTGTCCACCAGGGCCTCC
  ATGAGCCGACACATGCGGCGCAAGCACCCCGAGGTGCTCGCGGTGAGGATCGATGACCTGGACCACCTCC
  CGGAGACCACCACCATCGACGCCTCCTCCATTGGCATCGTCCAGCCTGAGCTGACTCTGGAGCAGGAGGA
  TTTGGCCGAAGGGAAGCACGGGAAAGCTGCCAAGCGAAGTCACAAGAGAAAGCAGAAGCCAGAAGAGGAG
  GCGGGTGCTCCGGTGCCCGAGGACGCCACCTTCAGCGAATACTCAGAGAAAGAGACGGAGTTCACAGGCA
  GTGTAGGCGACGAGACCAATTCCGCAGTACAGAGCATTCAGCAGGTAGTGGTGACCCTGGGTGACCCAAA
  TGTGACCACACCATCGAGCTCAGTCGGCTTAACCAACATCACCGTGACCCCCATCACCACTGCGGCCGCG
  ACTCAGTTTACCAATCTCCAGCCGGTGGCCGTGGGGCACCTTACCACCCCTGAACGCCAGTTACAGCTGG
  ACAACTCAATCCTGACCGTGACCTTTGATACCGTCAGCGGCTCTGCCATGTTGCACAACCGCCAAAATGA
  CGTCCAGATCCACCCCCAGCCGGAAGCCTCGAACCCACAGTCTGTGGCCCATTTCATCAACCTGACGACC
  CTGGTCAACTCCATCACGCCCCTGGGGAGCCAGCTTAGTGACCAGCACCCGCTCACGTGGCGGGCAGTGC
  CCCAGACTGACGTCTTGCCACCCTCGCAGCCGCAGGCACCCCCACAGCAGGCGGCCCAGCCCCAGGTGCA
  GGCGGAGCAGCAGCAGCAGCAGATGTACAGCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219769 representing NM_001040424
  Red=Cloning site Green=Tags(s)
  
  MPRRRPPASGAAQFPERIATRSPDPIPLCTFQRQVSEMAEDGSEEIMFIWCEDCSQYHDSECPELGPVVM
  VKDSFVLSRARSSLPPNLEIRRLEDGAEGVFAITQLVKRTQFGPFESRRVAKWEKESAFPLKVFQKDGHP
  VCFDTSNEDDCNWMMLVRPAAEAEHQNLTAYQHGSDVYFTTSRDIPPGTELRVWYAAFYAKKMDKPMLKQ
  AGSGVHAAGTPENSAPVESEPSQWACKVCSATFLELQLLNEHLLGHLEQAKSLPPGSQSEAAAPEKEQDT
  PRGEPPAVPESENVATKEQKKKPRRGRKPKVSKAEQPLVIVEDKEPTEQVAEIITEVPPDEPVSATPDER
  IMELVLGKLATTTTDTSSVPKFTHHQNNTITLKRSLILSSRHGIRRKLIKQLGEHKRVYQCNICSKIFQN
  SSNLSRHVRSHGDKLFKCEECAKLFSRKESLKQHVSYKHSRNEVDGEYRYRCGTCEKTFRIESALEFHNC
  RTDDKTFQCEMCFRFFSTNSNLSKHKKKHGDKKFACEVCSKMFYRKDVMLDHQRRHLEGVRRVKREDLEA
  GGENLVRYKKEPSGCPVCGKVFSCRSNMNKHLLTHGDKKYTCEICGRKFFRVDVLRDHIHVHFKDIALMD
  DHQREEFIGKIGISSEENDDNSDESADSEPHKYSCKRCQLTFGRGKEYLKHIMEVHKEKGYGCSICNRRF
  ALKATYHAHMVIHRENLPDPNVQKYIHPCEICGRIFNSIGNLERHKLIHTGVKSHACEQCGKSFARKDML
  KEHMRVHDNVREYLCAECGKGMKTKHALRHHMKLHKGIKEYECKECHRRFAQKVNMLKHCKRHTGIKDFM
  CELCGKTFSERNTMETHKLIHTVGKQWTCSVCDKKYVTEYMLQKHVQLTHDKVEAQSCQLCGTKVSTRAS
  MSRHMRRKHPEVLAVRIDDLDHLPETTTIDASSIGIVQPELTLEQEDLAEGKHGKAAKRSHKRKQKPEEE
  AGAPVPEDATFSEYSEKETEFTGSVGDETNSAVQSIQQVVVTLGDPNVTTPSSSVGLTNITVTPITTAAA
  TQFTNLQPVAVGHLTTPERQLQLDNSILTVTFDTVSGSAMLHNRQNDVQIHPQPEASNPQSVAHFINLTT
  LVNSITPLGSQLSDQHPLTWRAVPQTDVLPPSQPQAPPQQAAQPQVQAEQQQQQMYSY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040424
• ORF Size: 3534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040424.2
• RefSeq Size: 6654 bp
• RefSeq ORF: 3426 bp
• Locus ID: 63977
• UniProt ID: P57071
• Cytogenetics: 21q22.3
• Gene Summary: Sequence-specific DNA-binding transcriptional regulator. Plays a role as a molecular node in a transcriptional network regulating embryonic development and cell fate decision. Stimulates the expression of upstream key transcriptional activators and repressors of the Wnt/beta-catenin and MAPK/ERK pathways, respectively, that are essential for naive pluripotency and self-renewal maintenance of embryonic stem cells (ESCs). Specifically promotes SPRY1 and RSPO1 transcription activation through recognition and direct binding of a specific DNA sequence in their promoter regions. Involved in early embryo development (By similarity). Plays also a role in induced pluripotent stem cells (iPSCs) reprogramming (PubMed:28740264).[UniProtKB/Swiss-Prot Function]