PPAP2A (PLPP1) (NM_176895) Human Untagged Clone

CAT#: SC310573

PPAP2A (untagged)-Human phosphatidic acid phosphatase type 2A (PPAP2A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPAP2A
• Synonyms: LLP1a; LPP1; PAP-2a; PAP2; PPAP2A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_176895 edited
  CAACCGGGGCAGGCCGTGCCGGCTGAGGAGGTCCTGAGGCTACAGAGCTGCCGCGGCTGG
  CACACGAGCGCCTCGGCACTAACCGAGTGTTCGCGGGGGCTGTGAGGGGAGGGCCCCGGG
  CGCCATTGCTGGCGGTGGGAGCGCCGCCCGGTCTCAGCCCGCCCTCGGCTGCTCTCCTCC
  TCCGGCTGGGAGGGGCCGTAGCTCGGGGCCGTCGCCAGCCCCGGCCCGGGCTCGAGAATC
  AAGGGCCTCGGCCGCCGTCCCGCAGCTCAGTCCATCGCCCTTGCCGGGCAGCCCGGGCAG
  AGACCATGTTTGACAAGACGCGGCTGCCGTACGTGGCCCTCGATGTGCTCTGCGTGTTGC
  TGGCTTCCATGCCTATGGCTGTTCTAAAATTGGGCCAAATATATCCATTTCAGAGAGGCT
  TTTTCTGTAAAGACAACAGCATCAACTATCCGTACCATGACAGTACCGTCACATCCACTG
  TCCTCATCCTAGTGGGGGTTGGCTTGCCCATTTCCTCTATTATTCTTGGAGAAACCCTGT
  CTGTTTACTGTAACCTTTTGCACTCAAATTCCTTTATCAGGAATAACTACATAGCCACTA
  TTTACAAAGCCATTGGAACCTTTTTATTTGGTGCAGCTGCTAGTCAGTCCCTGACTGACA
  TTGCCAAGTATTCAATAGGCAGACTGCGGCCTCACTTCTTGGATGTTTGTGATCCAGATT
  GGTCAAAAATCAACTGCAGCGATGGTTACATTGAATACTACATATGTCGAGGGAATGCAG
  AAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGGCCACTCTTCGTTTTCCATGTACT
  GCATGCTGTTTGTGGCACTTTATCTTCAAGCCAGGATGAAGGGAGACTGGGCAAGACTCT
  TACGCCCCACACTGCAATTTGGTCTTGTTGCCGTATCCATTTATGTGGGCCTTTCTCGAG
  TTTCTGATTATAAACACCACTGGAGCGATGTGTTGACTGGACTCATTCAGGGAGCTCTGG
  TTGCAATATTAGTTGCTGTATATGTATCGGATTTCTTCAAAGAAAGAACTTCTTTTAAAG
  AAAGAAAAGAGGAGGACTCTCATACAACTCTGCATGAAACACCAACAACTGGGAATCACT
  ATCCGAGCAATCACCAGCCTTGAAAGGCAGCAGGGTGCCCAGGTGAAGCTGGCCTGTTTT
  CTAAAGGAAAATGATTGCCACAAGGCAAGAGGATGCATCTTTCTTCCTGGTGTACAAGCC
  TTTAAAGACTTCTGCTGCTGCTATGCCTCTTGGATGCACACTTTGTGTGTACATAGTTAC
  CTTTAACTCAGTGGTTATCTAATAGCTCTAAACTCATTAAAAAAACTCCAAGCCTTCCAC
  CAAAACAGTGCCCCACCTGTATACATTTTTATTAAAAAAATGTAATGCTTATGTATAAAC
  ATGTATGTAATATGCTTTCTATGAATGATGTTTGATTTAAATATAATACATATTAAAATG
  TATGGGRGAACCAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_176895 unedited
  NGTCAAATATTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACCAGGCAACCGGGG
  CAGGCCGTGCCGGCTGAGGAGGTCCTGAGGCTACAGAGCTGCCGCGGCTGGCACACGAGC
  GCCTCGGCACTAACCGAGTGTTCGCGGGGGCTGTGAGGGGAGGGCCCCGGGCGCCATTGC
  TGGCGGTGGGAGCGCCGCCCGGTCTCAGCCCGCCCTCGGCTGCTCTCCTCCTCCGGCTGG
  GAGGGGCCGTAGCTCGGGGCCGTCGCCAGCCCCGGCCCGGGCTCGAGAATCAAGGGCCTC
  GGCCGCCGTCCCGCAGCTCAGTCCATCGCCCTTGCCGGGCAGCCCGGGCAGAGACCATGT
  TTGACAAGACGCGGCTGCCGTACGTGGCCCTCGATGTGCTCTGCGTGTTGCTGGCTTCCA
  TGCCTATGGCTGTTCTAAAATTGGGCCAAATATATCCATTTCAGAGAGGCTTTTTCTGTA
  AAGACAACAGCATCAACTATCCGTACCATGACAGTACCGTCACATCCACTGTCCTCATCC
  TAGTGGGGGTTGGCTTGCCCATTTCCTCTATTATTCTTGGAGAAACCCTGTCTGTTTACT
  GTAACCTTTTGCACTCAAATTCCTTTATCANGAATAACTACATAGCCACTATTTACAAAG
  CCATTGGAACCTTTTTTATTGGTGCAGCTGCTAGTCAGTCCCTGACTGACATTGCCAAGT
  ATTTCATAGGCAGACTGCGGCCTCACTTCTGGNATGTTTGTGATCCAGATTGGTCAAAAA
  TCAACTGCAGCGATGGGTACATTGAATACTACATATGTCGAGGGAATGCANAANGAGTTN
  ANGNANGGCAGNTGTTCTTTCTANCAGGCCACCTCTTCGTTTCCATGGTACTGCATGCCT
  GTTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_176895 unedited
  CCCCATGGGGGATTGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGATG
  CCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTT
  TTTTTTTTTTTTTTTTTGGTTCTCCCATACATTTTAATATGTATTATATTTAAATCAAAC
  ATCATTCATAGAAAGCATATTACATACATGTTTATACATAAGCATTACATTTTTTTAATA
  AAAATGTATACAGGTGGGGCACTGTTTTGGTGGAAGGCTTGGAGTTTTTTTAATGAGTTT
  AGAGCTATTAGATAACCACTGAGTTAAAGGTAACTATGTACACACAAAGTGTGCATCCAA
  GAGGCATAGCAGCAGCAGAAGTCTTTAAAGGCTTGTACACCAGGAAGAAAGATGCATCCT
  CTTGCCTTGTGGCAATCATTTTCCTTTAGAAAACAGGCCAGCTTCACCTGGGCACCCTGC
  TGCCTTTCAAGGCTGGTGATTGCTCGGATAGTGATTCCCAGTTGTTGGTGTTTCATGCAG
  AGTTGTATGAGAGTCCTCCTCTTTTCTTTCTTTAAAAGAAGTTCTTTCTTTGAAGAAATC
  CGATACATATACAGCAACTAATATTGCAACCAGAGCTCCCTGAATGAGTCCAGTCAACAC
  ATCGCTCCAGTGGTGTTTATAATCAGAAACTCGAGAAAGGCCCACATAAATGGATACGGC
  AACAAGACCAAATTGCAGTGTGGNGCGTAAGAGTCTTGCCCAGNTCTCCTTCATCCTGGC
  TTTGAAGATAAGTGCCACAAACAGCATGCAGTACATGGAAAACGAANAGTGGCCTGAATA
  GAANGGACACCTGCCTTTCTTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_176895
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176895.1, NP_795714.1
• RefSeq Size: 1641 bp
• RefSeq ORF: 858 bp
• Locus ID: 8611
• UniProt ID: O14494
• Cytogenetics: 5q11.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Sphingolipid metabolism
• Gene Summary: The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in synthesis of glycerolipids and in phospholipase D-mediated signal transduction. This enzyme is an integral membrane glycoprotein that plays a role in the hydrolysis and uptake of lipids from extracellular space. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
  Transcript Variant: This variant (2, also named PAP2-a2) encodes the longer isoform (2).