CDK3 (NM_001258) Human Untagged Clone

CAT#: SC310548

CDK3 (untagged)-Human cyclin-dependent kinase 3 (CDK3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310548 representing NM_001258.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATATGTTCCAGAAGGTAGAGAAGATCGGAGAGGGCACCTATGGGGTGGTGTACAAGGCCAAGAAC
  AGGGAGACAGGGCAGCTGGTGGCCCTGAAGAAGATCAGACTGGATTTGGAGATGGAGGGGGTCCCAAGC
  ACTGCCATCAGGGAGATCTCGCTGCTCAAGGAACTGAAGCACCCCAACATCGTCCGACTGCTGGACGTG
  GTGCACAACGAGAGGAAGCTCTATCTGGTGTTTGAGTTCCTCAGCCAGGACCTGAAGAAGTACATGGAC
  TCCACCCCAGGCTCAGAGCTCCCCCTGCACCTCATCAAGAGCTACCTCTTCCAGCTGCTGCAGGGGGTG
  AGTTTCTGCCACTCACATCGGGTCATCCACCGAGACCTGAAGCCCCAGAACCTGCTCATCAATGAGTTG
  GGTGCCATCAAGCTGGCTGACTTCGGCCTGGCTCGCGCCTTCGGGGTGCCCCTGCGCACCTACACCCAT
  GAGGTGGTGACACTGTGGTATCGCGCCCCCGAGATTCTCTTGGGCAGCAAGTTCTATACCACAGCTGTG
  GATATCTGGAGCATTGGTTGCATCTTTGCAGAGATGGTGACTCGAAAAGCCCTGTTTCCTGGTGACTCT
  GAGATTGACCAGCTCTTTCGTATCTTTCGTATGCTGGGGACACCCAGCGAAGACACATGGCCCGGGGTC
  ACCCAGCTGCCTGACTATAAGGGCAGCTTCCCTAAGTGGACCAGGAAGGGACTGGAAGAGATTGTGCCC
  AATCTGGAGCCAGAGGGCAGGGACCTGCTCATGCAACTCCTGCAGTATGACCCCAGCCAGCGGATCACA
  GCCAAGACTGCCCTGGCCCACCCGTACTTCTCATCCCCTGAGCCCTCCCCAGCTGCCCGCCAGTATGTG
  CTGCAGCGATTCCGCCATTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001258
• Insert Size: 918 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258.2
• RefSeq Size: 1584 bp
• RefSeq ORF: 918 bp
• Locus ID: 1018
• UniProt ID: Q00526
• Cytogenetics: 17q25.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 35 kDa
• Gene Summary: This gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. [provided by RefSeq, Jul 2008]