PRR5-ARHGAP8 (NM_181334) Human Untagged Clone

CAT#: SC310356

PRR5 (untagged)-Human PRR5-ARHGAP8 readthrough (PRR5-ARHGAP8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRR5-ARHGAP8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310356 representing NM_181334.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGGACTCTCCGCAGGTTGAAGTTCATGAGTTCGCCCAGCCTCAGTGACCTGGGCAAGAGAGAGCCG
  GCCGCCGCCGCGGACGAGCGGGGCACGCAGCAGCGCCGGGCCTGCGCCAACGCCACCTGGAACAGCATC
  CACAACGGGGTGATCGCCGTCTTCCAGCGCAAGGGGCTGCCCGACCAGGAGCTCTTCAGCCTCAACGAG
  GGCGTCCGGCAGCTGTTGAAGACAGAGCTGGGGTCCTTCTTCACGGAGTACCTGCAGAACCAGCTGCTG
  ACAAAAGGCATGGTGATCCTTCGGGACAAGATTCGCTTCTATGAGGAGCTGCAGAGAGACAAGGCGGCG
  GCGGCTGCTGTGCTGGGTGCAGTGAGGAAGAGGCCCTCGGTGGTGCCCATGGCTGGCCAGGATCCTGCG
  CTGAGCACGAGTCACCCGTTCTACGACGTGGCCAGACATGGCATTCTGCAGGTGGCAGGGGATGACCGC
  TTTGGAAGACGTGTTGTCACGTTCAGCTGCTGCCGGATGCCACCCTCCCACGAGCTGGACCACCAGCGG
  CTGCTGGAGTATTTGAAGTACACACTGGACCAATACGTTGAGAACGATTATACCATCGTCTATTTCCAC
  TACGGGCTGAACAGCCGGAACAAGCCTTCCCTGGGCTGGCTCCAGAGCGCATACAAGGAGTTCGATAGG
  AAGTACAAGAAGAACTTGAAGGCCCTCTACGTGGTGCACCCCACCAGCTTCATCAAGGTCCTGTGGAAC
  ATCTTGAAGCCCCTCATCAGTCACAAGTTTGGGAAGAAAGTCATCTATTTCAACTACCTGAGTGAGCTC
  CACGAACACCTTAAATACGACCAGCTGGTCATCCCTCCCGAAGTTTTGCGGTACGATGAGAAGCTCCAG
  AGCCTGCACGAGGGCCGGACGCCGCCTCCCACCAAGACACCACCGCCGCGGCCCCCGCTGCCCACACAG
  CAGTTTGGCGTCAGTCTGCAATACCTCAAAGACAAAAATCAAGGCGAACTCATCCCCCCTGTGCTGAGG
  TTCACAGTGACGTACCTGAGAGAGAAAGGCCTGCGCACCGAGGGCCTGTTCCGGAGATCCGCCAGCGTG
  CAGACCGTCCGCGAGATCCAGAGGCTCTACAACCAAGGGAAGCCCGTGAACTTTGACGACTACGGGGAC
  ATTCACATCCCTGCCGTGATCCTGAAGACCTTCCTGCGAGAGCTGCCCCAGCCGCTTCTGACCTTCCAG
  GCCTACGAGCAGATTCTCGGGATCACCTGTGTGGAGAGCAGCCTGCGTGTCACTGGCTGCCGCCAGATC
  TTACGGAGCCTCCCAGAGCACAACTACGTCGTCCTCCGCTACCTCATGGGCTTCCTGCATGCGGTGTCC
  CGGGAGAGCATCTTCAACAAAATGAACAGCTCTAACCTGGCCTGTGTCTTCGGGCTGAATTTGATCTGG
  CCATCCCAGGGGGTCTCCTCCCTGAGTGCCCTTGTGCCCCTGAACATGTTCACTGAACTGCTGATCGAG
  TACTATGAAAAGATCTTCAGCACCCCGGAGGCACCTGGGGAGCACGGCCTGGCACCATGGGAACAGGGG
  AGCAGGGCAGCCCCTTTGCAGGAGGCTGTGCCACGGACACAAGCCACGGGCCTCACCAAGCCTACCCTA
  CCTCCGAGTCCCCTGATGGCAGCCAGAAGACGTCTCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_181334
• Insert Size: 1695 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181334.5
• RefSeq Size: 2161 bp
• RefSeq ORF: 1695 bp
• Locus ID: 553158
• Cytogenetics: 22q13.31
• MW: 64.5 kDa
• Gene Summary: The PRR5-ARHGAP8 mRNA is an infrequent but naturally occurring read-through transcript of the neighboring proline rich 5, renal (PRR5) and Rho GTPase activating protein 8 (ARHGAP8) genes. The resulting fusion protein contains sequence identity with each individual gene product, and it includes domains characteristic of a RhoGAP protein. The significance of this read-through transcript and the function of its protein product have not yet been determined. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1) of this protein.