ATF7 (NM_006856) Human Untagged Clone

CAT#: SC310340

ATF7 (untagged)-Human activating transcription factor 7 (ATF7), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATF7
• Synonyms: ATFA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310340 representing NM_006856.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGAGACGACAGACCGTTTGTGTGCAATGCCCCGGGCTGTGGACAGAGATTTACAAACGAGGACCAC
  CTGGCAGTTCATAAACACAAGCATGAGATGACATTGAAATTTGGCCCAGCCCGAACTGACTCAGTCATC
  ATTGCAGATCAAACGCCTACTCCAACTAGATTCCTGAAGAACTGTGAGGAGGTGGGACTCTTCAATGAA
  CTAGCTAGCTCCTTTGAACATGAATTCAAGAAAGCTGCAGATGAGGATGAGAAAAAGGCTGCTGCTGGG
  CCCCTTGACATGTCTCTGCCTTCCACACCAGACATCAAAATCAAAGAAGAAGAGCCAGTGGAGGTAGAC
  TCATCCCCACCTGATAGCCCTGCCTCTAGTCCCTGTTCCCCACCACTGAAGGAGAAGGAGGTTACCCCA
  AAGCCTGTTCTGATCTCTACCCCCACACCCACCATTGTACGTCCTGGCTCCCTGCCTCTCCACTTGGGC
  TATGATCCACTTCATCCAACCCTTCCCTCCCCAACCTCTGTCATCACACAGGCTCCACCATCCAACAGG
  CAAATGGGGTCTCCCACTGGCTCCCTCCCTCTTGTCATGCATCTTGCTAATGGACAGACCATGCCTGTG
  TTGCCAGGGCCTCCAGTACAGATGCCGTCTGTTATATCGCTGGCCAGACCTGTGTCCATGGTGCCCAAC
  ATTCCTGGTATCCCTGGCCCACCAGTTAACAGTAGTGGCTCCATTTCTCCCTCTGGCCACCCTATACCA
  TCAGAAGCCAAGATGAGACTGAAAGCCACCCTAACTCACCAAGTCTCCTCAATCAATGGTGGTTGTGGA
  ATGGTGGTGGGTACTGCCAGCACCATGGTGACAGCCCGCCCAGAGCAGAGCCAGATTCTCATCCAGCAC
  CCTGATGCCCCATCCCCTGCCCAGCCACAGGTCTCACCAGCTCAGCCCACCCCTAGTACTGGGGGGCGA
  CGGCGGCGCACAGTAGATGAAGATCCAGATGAGCGACGGCAGCGCTTTCTGGAGCGCAACCGGGCTGCA
  GCCTCCCGCTGCCGCCAAAAGCGAAAGCTGTGGGTGTCCTCCCTAGAGAAGAAGGCCGAAGAACTCACT
  TCTCAGAACATTCAGCTGAGTAATGAAGTCACATTACTACGCAATGAGGTGGCCCAGTTGAAACAGCTA
  CTGTTAGCTCATAAAGACTGCCCAGTCACTGCACTACAGAAAAAGACTCAAGGCTATTTAGAAAGCCCC
  AAGGAAAGCTCAGAGCCAACGGGTTCTCCAGCCCCTGTGATTCAGCACAGCTCAGCAACAGCCCCTAGC
  AATGGCCTCAGTGTTCGCTCTGCAGCTGAAGCTGTGGCCACCTCGGTCCTCACTCAGATGGCCAGCCAA
  AGGACAGAACTGAGCATGCCGATACAATCGCATGTAATCATGACCCCACAGTCCCAGTCTGCGGGCAGA
  TGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006856
• Insert Size: 1452 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006856.2
• RefSeq Size: 6704 bp
• RefSeq ORF: 1452 bp
• Locus ID: 11016
• UniProt ID: P17544
• Cytogenetics: 12q13.13
• Domains: BRLZ, zf-C2H2
• Protein Families: Transcription Factors
• MW: 51.8 kDa
• Gene Summary: Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2), as well as variants 8 and 9, encodes isoform 2 (also known as ATFa2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.