CYP2C18 (NM_000772) Human Untagged Clone

CAT#: SC310335

CYP2C18 (untagged)-Human cytochrome P450, family 2, subfamily C, polypeptide 18 (CYP2C18), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP2C18
• Synonyms: CPCI; CYP2C; CYP2C17; P450-6B/29C; P450IIC17
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000772 edited
  TCAAGGAAAAACAACAACAACAACAACAAAATCCTGGGCTCTGCTTCAGACTAGTTAAAC
  CAGAATCTCCAGGGTGGGGCACCGGAAAGAACAAGAAAAAAGAACACCTTATTTTTATCT
  TCTTCAGTGAGCCAATGTTCATTCAAAAGAGAGATTAAAGTGCTTTTTGCTGACTAGTCA
  CAGTCAGAGTCAGAATCACAGGTGGATTAGTAGGGAGTGTTATAAAAGCCTTGAAGTGAA
  AGCCCGCAGTTGTCTTACTAAGAAGAGAAGCCTTCAATGGATCCAGCTGTGGCTCTGGTG
  CTCTGTCTCTCCTGTTTGTTTCTCCTTTCACTCTGGAGGCAGAGCTCTGGAAGAGGGAGG
  CTCCCGTCTGGCCCCACTCCTCTCCCGATTATTGGAAATATCCTGCAGTTAGATGTTAAG
  GACATGAGCAAATCCTTAACCAATTTCTCAAAAGTCTATGGCCCTGTGTTCACTGTGTAT
  TTTGGCCTGAAGCCCATTGTGGTGTTGCATGGATATGAAGCAGTGAAGGAGGCCCTGATT
  GATCATGGAGAGGAGTTTTCTGGAAGAGGAAGTTTTCCAGTGGCTGAAAAAGTTAACAAA
  GGACTTGGAATCCTTTTCAGCAATGGAAAGAGATGGAAGGAGATCCGGCGTTTCTGCCTC
  ATGACTCTGCGGAATTTTGGGATGGGGAAGAGGAGCATCGAGGACCGTGTTCAAGAGGAA
  GCCCGCTGCCTTGTGGAGGAGTTGAGAAAAACCAATGCCTCACCCTGTGATCCCACTTTC
  ATCCTGGGCTGTGCTCCCTGCAATGTGATCTGCTCTGTTATTTTCCATGATCGATTTGAT
  TATAAAGATCAGAGGTTTCTTAACTTGATGGAAAAATTCAATGAAAACCTCAGGATTCTG
  AGCTCTCCATGGATCCAGGTCTGCAATAATTTCCCTGCTCTCATCGATTATCTCCCAGGA
  AGTCATAATAAAATAGCTGAAAATTTTGCTTACATTAAAAGTTATGTATTGGAGAGAATA
  AAAGAACATCAAGAATCCCTGGACATGAACAGTGCTCGGGACTTTATTGATTGTTTCCTG
  ATCAAAATGGAACAGGAAAAGCACAATCAACAGTCTGAATTTACTGTTGAAAGCTTGATA
  GCCACTGTAACTGATATGTTTGGGGCTGGAACAGAGACAACGAGCACCACTCTGAGATAT
  GGACTCCTGCTCCTGCTGAAGTACCCAGAGGTCACAGCTAAAGTCCAGGAAGAGATTGAA
  TGTGTAGTTGGCAGAAACCGGAGCCCCTGTATGCAGGACAGGAGTCACATGCCCTACACA
  GATGCTGTGGTGCACGAGATCCAGAGATACATTGACCTCCTCCCCACCAACCTGCCCCAT
  GCAGTGACCTGTGATGTTAAATTCAAAAACTACCTCATCCCCAAGGGCACGACCATAATA
  ACATCCCTGACTTCTGTGCTGCACAATGACAAAGAATTCCCCAACCCAGAGATGTTTGAC
  CCTGGCCACTTTCTGGATAAGAGTGGCAACTTTAAGAAAAGTGACTACTTCATGCCTTTC
  TCAGCAGGAAAACGGATGTGTATGGGAGAGGGCCTGGCCCGCATGGAGCTGTTTTTATTC
  CTGACCACCATTTTGCAGAACTTTAACCTGAAATCTCAGGTTGACCCAAAGGATATTGAC
  ATCACCCCCATTGCCAATGCATTTGGTCGTGTGCCACCCTTGTACCAGCTCTGCTTCATT
  CCTGTCTGAAGAAGGGCAGATAGTTTGGCTGCTCCTGTGCTGTCACCTGCAATTCTCCCT
  TATCAGGGCCATTGGCCTCTCCCTTCTCTCTGTGAGGGATATTTTCTCTGACTTGTCAAT
  CCACATCTTCCCATTCCCTCAAGATCCAATGAACATCCAACCTCCATTAAAGAGAGTTTC
  TTGGGTCACTTCCTAAATATATCTGCTATTCTCCATACTCTGTATCACTTGTATTGACCA
  CCACATATGCTAATACCTATCTACTGCTGAGTTGTCAGTATGTTATCACTAGAAAACAAA
  GAAAAATGATTAATAAATGACAATTCAGAGCCAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >Reverse primer walk for NM_000772 unedited
  NGGGGCTGGTTACCAGGNAAACTCANATCTGNAGTTTTCATTGATTTTTCCATCAAGTTA
  AAAAACCTCTGATCTTTATAATCAAATCGATCATGGNAAAATAACAGAGCAGATCACATT
  GCAGGGAGCACAGCCCAGGATGAAAGTGGGATCACAGGGTGAGGCATTGGTTTTTCTCAA
  CTCCTCCACAAGGCAGCGGGCTTCCTCTTGAACACGGTCCTCGATGCTCCTCTTCCCCAT
  CCCAAAATTCCGCAGAGTCATGAGGCAGAAACGCCGGATCTCCTTCCATCTCTTTCCATT
  GCTGAAAAGGATTCCAAGTCCTTTGTTAACTTTTTCAGCCACTGGAAAACTTCCTCTTCC
  AGAAAACTCCTCTCCATGATCAATCAGGGCCTCCTTCACTGCTTCATATCCATGCAACAC
  CACAATGGGCTTCAGGCCAAAATACACAGTGAACACAGGGCCATAGACTTTTGAGAAATT
  GGTTAAGGATTTGCTCATGTCCTTAACATCTAACTGCAGGATATTTCCAATAATCGGGAG
  AGGAGTGGGGCCAGACGGGAGCCTCCCTCTTCCAGAGCTCTGCCTCCAGAGTGAAAGGAG
  AAACAAACAGGAGAGACAGAGCACCAGAGCCACAGCTGGATCCATTGAAGGCTTCTCTTC
  TTAGTAAGACAACTGCGGGCTTTCACTTCAAGGCTTTTATAACACTCCCTACTAATCCAC
  CTGTGATTCTGACTCTGACTGTGACTAGTCAGCANAAAGCACTTTAATCTCTCTTTTGAA
  TGAACATTGGCTCACTGAAGAAGATAAAATAAGGTGTTCTTTTTTCTTGGTCTTTCCGGT
  GCCCCACCCTGGAGATTCTGGTTTAACTAGTCTGAAGCAGAAGCCCAGATTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000772 unedited
  NGGCCCCATTGGNGAATGGCACTTTCCAGGNCCAGNANGAGCACTGGNGGNAGGGGTCAC
  AGGGNATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGT
  CGAGTTTTTTTTTTTTTTTTTTTTTTGGCTCTGAATTGTCATTTATTAATCATTTTTCTT
  TGTTTTCTAGTGATAACATACTGACAACTCAGCAGTAGATAGGTATTAACATATGTGGTG
  GTCAATACAAGTGATACAGAGTATGGAGAATAGCAGATATATTTAGGAAGTGACCCAAGA
  AACTCTCTTTAATGGAGGTTGGATGTTCATTGGATCTTGAGGGAATGGGAAGATGTGGAT
  TGACAAGTCAGAGAAAATATCCCTCACAGAGAGAAGGGAGAGGCCAATGGCCCTGATAAG
  GGAGAATTGCAGGTGACAGCACAGGAGCAGCCAAACTATCTGCCCTTCTTCAGACAGGAA
  TGAAGCAGAGCTGGTACAAGGGTGGCACACGACCAAATGCATTGGCAATGGGGGTGATGT
  CAATATCCTTTGGGTCAACCTGAGATTTCAGGTTAAAGTTCTGCAAAATGGTGGTCAGGA
  ATAAAAACAGCTCCATGCGGGCCAGGCCCTCTCCCATACACATCCGTTTTCCTGCTGAGA
  AAGGCATGAAGTAGTCACTTTTCTTAAAGTTGCCACTCTTATCCAGAAAGTGGCCAGGGT
  CAAACATCTCTGGGTTGGGGAATTCTTTGTCATTGTGCAGCACAGAAGTCAGGGATGTTA
  TTATGGTCGTGCCCTTGGGGATGAAGTAGTTTTTGAATTTAACATCACAGGTCACTGCAT
  GGGGCANGTTGGTGGGGAAGAAGTCAATGTATCTCTGGATCTCGTGCACCACAGCATCTC
  
• Restriction Sites: Please inquire
• ACCN: NM_000772
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000772.1, NP_000763.1
• RefSeq Size: 1995 bp
• RefSeq ORF: 1473 bp
• Locus ID: 1562
• UniProt ID: P33260
• Cytogenetics: 10q23.33
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum but its specific substrate has not yet been determined. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. An additional gene, CYP2C17, was once thought to exist; however, CYP2C17 is now considered an artefact based on a chimera of CYP2C18 and CYP2C19. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).