NT5C2 (NM_012229) Human Untagged Clone

CAT#: SC310267

NT5C2 (untagged)-Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NT5C2
• Synonyms: cN-II; GMP; NT5B; PNT5; SPG45; SPG65
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012229 edited
  GATTGTTGCCGTGCGCTGGAGCCGAGTTTCGCTCTGCTGCCCAGGCTGGAGTACAGTGAT
  GTGATCTCCTGACGTCAAGTGATCCATTCGCCTCGGCCTCCCAAAGTTCTGGGATTACAA
  GTAATACAAAATACAGTTAATAAAATGTCAACCTCCTGGAGTGATCGGTTACAGAATGCA
  GCAGATATGCCTGCTAACATGGATAAGCATGCCCTGAAAAAGTATCGTCGAGAAGCCTAT
  CATCGGGTGTTTGTGAACCGAAGTTTAGCAATGGAAAAGATAAAGTGTTTTGGTTTTGAT
  ATGGATTATACCCTTGCTGTGTACAAGTCCCCAGAGTATGAGTCCCTTGGTTTTGAGCTT
  ACTGTGGAGAGATTAGTTTCTATTGGCTATCCCCAGGAGTTGCTCAGCTTTGCTTATGAT
  TCTACATTCCCTACCAGGGGACTTGTCTTTGACACACTGTATGGAAATCTTTTGAAAGTC
  GATGCCTATGGAAACCTCTTGGTCTGTGCACATGGATTTAACTTTATAAGGGGACCAGAA
  ACTAGAGAACAGTATCCAAATAAATTTATCCAGCGAGATGATACTGAAAGATTTTACATT
  CTGAACACACTATTCAACCTACCAGAGACCTACCTGTTGGCCTGCCTAGTAGATTTTTTT
  ACTAATTGTCCCAGATATACCAGTTGTGAAACAGGATTTAAAGATGGGGACCTCTTCATG
  TCCTACCGGAGTATGTTCCAGGATGTAAGAGATGCTGTTGACTGGGTTCATTACAAGGGC
  TCCCTTAAGGAAAAGACAGTTGAAAATCTTGAGAAGTATGTAGTCAAAGATGGAAAACTG
  CCTTTGCTTCTGAGCCGGATGAAGGAAGTAGGGAAAGTATTTCTTGCTACCAACAGTGAC
  TATAAATATACAGATAAAATTATGACTTACCTGTTTGACTTCCCACATGGCCCCAAGCCT
  GGGAGCTCCCATCGACCATGGCAGTCCTACTTTGACTTGATCTTGGTGGATGCACGGAAA
  CCACTCTTTTTTGGAGAAGGCACAGTACTGCGTCAGGTGGATACTAAAACTGGCAAGCTG
  AAAATTGGTACCTACACAGGGCCCCTACAGCATGGTATCGTCTACTCAGGAGGTTCTTCT
  GATACGATCTGTGACCTGTTGGGAGCCAAGGGAAAAGACATTTTGTATATTGGAGATCAC
  ATTTTTGGGGACATTTTAAAATCAAAGAAACGGCAAGGGTGGCGAACTTTTTTGGTGATT
  CCTGAACTCGCACAGGAGCTACATGTCTGGACTGACAAGAGTTCACTTTTCGAAGAACTT
  CAGAGCTTGGATATTTTCTTGGCTGAACTCTACAAGCATCTTGACAGCAGTAGCAATGAG
  CGTCCAGACATCAGTTCCATCCAGAGACGTATTAAGAAAGTAACTCATGACATGGACATG
  TGCTATGGGATGATGGGAAGCCTGTTTCGCAGTGGCTCCCGGCAGACCCTTTTTGCCAGT
  CAAGTGATGCGTTATGCTGACCTCTATGCAGCATCTTTCATCAACCTGCTGTATTACCCT
  TTCAGCTACCTCTTCAGGGCTGCCCATGTCTTGATGCCTCATGAATCAACGGTGGAGCAC
  ACACACGTAGATATCAATGAGATGGAGTCTCCTCTTGCCACCCGGAACCGCACATCAGTG
  GATTTCAAAGACACTGACTACAAGCGGCACCAGCTGACACGGTCAATTAGTGAGATTAAA
  CCTCCCAACCTCTTCCCACTGGCCCCCCAGGAAATTACACACTGCCATGACGAAGATGAT
  GATGAAGAGGAGGAGGAGGAGGAAGAATAAGGAGGAAAACCAAAACCCCAAGCACCCATT
  AAACAAGTCCTGGCAGGACTCACAGGAACAAACGAGGTCCCTGTTAGGGTTCTACTCGGG
  GGAGGGAGGGGGCTCCATGAAAGGTACGTCTGAAAAGTTTCTGAAGATTTTATTATCATA
  GATACTTGTTTTGGTTTTGTGTATCTGTACTCTCTGCAGATGGTCCAAAATTGTAATGGA
  GTCTGTATTAGAAGAAAATAAGGGTAAAATCAGGCTGAACTGCATGTATATGGCTCCACT
  GTGGCTTGTGACACTTTTAAAATCATCCGTATGTCAGTGTATCTGGATACACGAGGAAAA
  GGAAAGAGTCTCAGAGTGGAACAAAGAGTGGGAAGAGGTGATCTGTAATGTTACAAATTG
  TGCTATTACTCCAAGGTCCAACTTTTCCAGTGCATTACATGGTATTGTATATCAGTGGAG
  AAATGTATTATTTCCATGATCAAATGTAGTCTCTGTTAAGGTCAAGTTTTCTTTTATAAG
  CCTTTAATTCATCCTCAGTGACTCTGGCAAGGCTGCTTCTCTATCACTGGCTTTGCACAG
  AAGTATGCTCTACTTGCGTTGCTTTAGGGCAGGATTCTATTTTGAGGGAAAAGACAGTAT
  CCTTATTACCTTTTGTTTGTTTAATAGCACAAATGCTTATTTGTTATCCAAAAACAACCT
  CCTTCTTATCTGTGATAAATCTATAGAAAGAATTTAGCTGCAAGTGGACAAAGGAACAAG
  CCCCCAGAAAAGAAAGGGAAGAACTGCCTTCTTATACTACAGAACATGCATTAGTGTGGG
  CTATATAGCTGTGGCTCATGCTACCCAATTCCAGATTTCTTTGTCCTCTAAGAGTTGATT
  GCTGTATATTAAAATTGAACATCAGAGGATGGGAAGAGGGCTCTGTAAGCCAGAACCTTA
  CTAAAGTAGAGGGCACAATCAGTGTGAATAAATTCACTTCAGAATCTCAAGTCAAGGCCA
  GGCACGGCGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGACAGGCGGATCACC
  TGAGGTCGGGAGTTCGAGACCAGCCTTACCAACATGGAGAAACCCCATCTCTACTAAAAA
  TACAAAATTACCTGGGCGTGGTGGTGCATGCCTGTAATCCCATCATCTACTCAGGAGGCT
  GAGGCAGGAGAATTGCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCCAGGATTGTGCCA
  TTGCACTCCAGCCTGGGCAACAAGAACAAAACTCCATCTCAAAAAATAAAAATCCCAATC
  CCAAGTCGAAATCACCTCTTGTTTTAAACAAGAATGAATCATTACTGTGTATGTTAGGGT
  ATTAAAACTGTTTCACCAGTACAGTGAAAGTTGTTTCAACATTTTAAACAAACAGTGGTT
  ATAGACTCTTTCTTTAACCATTGTATATTTTCTTCCATTCTTGTCATTGGTCAATAGGGG
  AGGGTAGATTAGCTGCTCCAGAATTCAATAAAGTGTAATATTTCTAAAAAAAAAAAAAAA
  AAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_012229
• Insert Size: 3400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_012229.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012229.2, NP_036361.1
• RefSeq Size: 3364 bp
• RefSeq ORF: 1686 bp
• Locus ID: 22978
• UniProt ID: P49902
• Cytogenetics: 10q24.32-q24.33
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
• Gene Summary: This gene encodes a hydrolase that serves as an important role in cellular purine metabolism by acting primarily on inosine 5'-monophosphate and other purine nucleotides. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1-3 encode the same protein (isoform 1).