NT5C2 (NM_012229) Human Tagged ORF Clone

CAT#: RC200194

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NT5C2 (Myc-DDK-tagged)-Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1



  "NM_012229" in other vectors (4)

Reconstitution Protocol

USD 523.00

USD 784.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NT5C2
Synonyms cN-II; GMP; NT5B; PNT5; SPG45; SPG65
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200194 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAACCTCCTGGAGTGATCGGTTACAGAATGCAGCAGATATGCCTGCTAACATGGATAAGCATGCCC
TGAAAAAGTATCGTCGAGAAGCCTATCATCGGGTGTTTGTGAACCGAAGTTTAGCAATGGAAAAGATAAA
GTGTTTTGGTTTTGATATGGATTATACCCTTGCTGTGTACAAGTCCCCAGAGTATGAGTCCCTTGGTTTT
GAGCTTACTGTGGAGAGATTAGTTTCTATTGGCTATCCCCAGGAGTTGCTCAGCTTTGCTTATGATTCTA
CATTCCCTACCAGGGGACTTGTCTTTGACACACTGTATGGAAATCTTTTGAAAGTCGATGCCTATGGAAA
CCTCTTGGTCTGTGCACATGGATTTAACTTTATAAGGGGACCAGAAACTAGAGAACAGTATCCAAATAAA
TTTATCCAGCGAGATGATACTGAAAGATTTTACATTCTGAACACACTATTCAACCTACCAGAGACCTACC
TGTTGGCCTGCCTAGTAGATTTTTTTACTAATTGTCCCAGATATACCAGTTGTGAAACAGGATTTAAAGA
TGGGGACCTCTTCATGTCCTACCGGAGTATGTTCCAGGATGTAAGAGATGCTGTTGACTGGGTTCATTAC
AAGGGCTCCCTTAAGGAAAAGACAGTTGAAAATCTTGAGAAGTATGTAGTCAAAGATGGAAAACTGCCTT
TGCTTCTGAGCCGGATGAAGGAAGTAGGGAAAGTATTTCTTGCTACCAACAGTGACTATAAATATACAGA
TAAAATTATGACTTACCTGTTTGACTTCCCACATGGCCCCAAGCCTGGGAGCTCCCATCGACCATGGCAG
TCCTACTTTGACTTGATCTTGGTGGATGCACGGAAACCACTCTTTTTTGGAGAAGGCACAGTACTGCGTC
AGGTGGATACTAAAACTGGCAAGCTGAAAATTGGTACCTACACAGGGCCCCTACAGCATGGTATCGTCTA
CTCAGGAGGTTCTTCTGATACGATCTGTGACCTGTTGGGAGCCAAGGGAAAAGACATTTTGTATATTGGA
GATCACATTTTTGGGGACATTTTAAAATCAAAGAAACGGCAAGGGTGGCGAACTTTTTTGGTGATTCCTG
AACTCGCACAGGAGCTACATGTCTGGACTGACAAGAGTTCACTTTTCGAAGAACTTCAGAGCTTGGATAT
TTTCTTGGCTGAACTCTACAAGCATCTTGACAGCAGTAGCAATGAGCGTCCAGACATCAGTTCCATCCAG
AGACGTATTAAGAAAGTAACTCATGACATGGACATGTGCTATGGGATGATGGGAAGCCTGTTTCGCAGTG
GCTCCCGGCAGACCCTTTTTGCCAGTCAAGTGATGCGTTATGCTGACCTCTATGCAGCATCTTTCATCAA
CCTGCTGTATTACCCTTTCAGCTACCTCTTCAGGGCTGCCCATGTCTTGATGCCTCATGAATCAACGGTG
GAGCACACACACGTAGATATCAATGAGATGGAGTCTCCTCTTGCCACCCGGAACCGCACATCAGTGGATT
TCAAAGACACTGACTACAAGCGGCACCAGCTGACACGGTCAATTAGTGAGATTAAACCTCCCAACCTCTT
CCCACTGGCCCCCCAGGAAATTACACACTGCCATGACGAAGATGATGATGAAGAGGAGGAGGAGGAGGAA
GAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200194 protein sequence
Red=Cloning site Green=Tags(s)

MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGF
ELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNK
FIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHY
KGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQ
SYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIG
DHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQ
RRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTV
EHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEE
E

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012229
ORF Size 1686 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012229.4
RefSeq Size 3551 bp
RefSeq ORF 1686 bp
Locus ID 22978
UniProt ID P49902
Cytogenetics 10q24.32-q24.33
Protein Pathways Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
MW 65 kDa
Gene Summary This gene encodes a hydrolase that serves as an important role in cellular purine metabolism by acting primarily on inosine 5'-monophosphate and other purine nucleotides. [provided by RefSeq, Oct 2011]

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