ACAP3 (NM_030649) Human Untagged Clone

CAT#: SC310127

ACAP3 (untagged)-Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 3 (ACAP3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACAP3
• Synonyms: CENTB5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310127 representing NM_030649.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACCGTGGAGTTCGAGGAGTGCGTCAAGGACTCCCCGCGCTTCAGGGCGACCATTGACGAGGTGGAG
  ACGGACGTGGTGGAGATTGAGGCCAAACTGGACAAGCTGGTGAAGCTGTGCAGTGGCATGGTGGAAGCC
  GGTAAGGCCTACGTCAGCACCAGCAGGCTTTTCGTGAGCGGCGTCCGCGACCTGTCCCAGCAGTGCCAG
  GGCGACACCGTCATCTCGGAATGTCTGCAGAGGTTCGCTGACAGCCTACAGGAGGTGGTGAACTACCAC
  ATGATCCTGTTTGACCAGGCCCAGAGGTCCGTGCGGCAGCAGCTCCAGAGCTTTGTCAAAGAGGATGTG
  CGGAAGTTCAAGGAGACAAAGAAGCAGTTTGACAAGGTGCGGGAGGACCTGGAGCTGTCCCTGGTGAGG
  AACGCCCAGGCCCCGAGGCACCGGCCCCACGAGGTGGAGGAAGCCACCGGGGCCCTCACCCTCACCAGG
  AAGTGCTTCCGCCACCTGGCACTGGACTATGTGCTCCAGATCAATGTTCTGCAGGCCAAGAAGAAGTTT
  GAGATCCTGGACTCTATGCTGTCCTTCATGCACGCCCAGTCCAGCTTCTTCCAGCAGGGCTACAGCCTC
  CTGCACCAGCTGGACCCCTACATGAAGAAGCTGGCAGCCGAGCTGGACCAGCTGGTGATCGACTCTGCG
  GTGGAAAAGCGTGAGATGGAGCGAAAGCACGCCGCCATCCAGCAGCGGACGCTGCTGCAGGACTTCTCC
  TACGATGAGTCCAAAGTGGAGTTTGACGTGGACGCGCCCAGTGGGGTGGTGATGGAGGGCTACCTCTTC
  AAGAGGGCCAGCAACGCTTTCAAGACATGGAACCGGCGCTGGTTCTCCATTCAGAACAGCCAGCTGGTC
  TACCAGAAGAAGCTCAAGGATGCCCTCACCGTGGTGGTGGATGACCTCCGCCTGTGCTCTGTGAAGCCG
  TGTGAGGACATCGAGCGGAGGTTCTGCTTCGAGGTGCTGTCACCCACCAAGAGCTGCATGCTGCAGGCT
  GACTCCGAGAAGCTGCGGCAAGCCTGGGTCCAGGCTGTGCAGGCCAGCATCGCCTCCGCCTACCGCGAG
  AGCCCTGACAGTTGCTATAGCGAGAGGCTGGACCGCACAGCATCCCCGTCCACGAGCAGCATCGACTCC
  GCCACCGACACTCGGGAGCGTGGCGTGAAGGGCGAGAGTGTGCTGCAGCGTGTGCAGAGTGTGGCCGGC
  AACAGCCAGTGCGGCGACTGCGGCCAGCCGGACCCCCGCTGGGCCAGCATCAACCTGGGCGTGCTGCTC
  TGCATTGAGTGCTCCGGCATCCACAGGAGCCTGGGTGTCCACTGCTCCAAGGTGCGGTCCCTGACGCTG
  GACTCGTGGGAGCCTGAGCTGCTAAAGCTGATGTGTGAGCTTGGAAACAGCGCTGTGAATCAGATCTAT
  GAGGCCCAGTGTGAGGGTGCAGGCAGCAGGAAACCCACAGCCAGCAGCTCCCGGCAGGACAAGGAGGCC
  TGGATCAAGGACAAATACGTGGAAAAGAAGTTTCTGCGGAAGGCGCCCATGGCACCAGCCCTGGAGGCC
  CCAAGACGCTGGAGGGTGCAGAAGTGCCTGCGGCCCCACAGCTCTCCCCGCGCTCCCACTGCCCGCCGC
  AAGGTCCGGCTTGAGCCCGTTCTGCCCTGTGTGGCCGCTCTGTCCTCAGTGGGCACCCTGGATCGTAAG
  TTCCGCCGAGACTCCCTCTTCTGTCCCGACGAGCTGGACTCGCTCTTCTCCTACTTCGACGCAGGGGCC
  GCAGGGGCTGGCCCTCGCAGTCTGAGTAGCGACAGTGGCCTTGGGGGCAGCTCGGATGGCAGCTCGGAC
  GTCCTGGCTTTCGGCTCGGGCTCTGTGGTGGACAGCGTCACTGAGGAGGAGGGTGCAGAGTCGGAGGAG
  TCCAGCGGTGAGGCAGACGGGGACACTGAGGCCGAGGCCTGGGGCCTGGCGGACGTGCGCGAGCTGCAC
  CCGGGGCTCTTGGCGCACCGCGCAGCGCGTGCCCGCGACCTTCCTGCGCTGGCGGCGGCGCTGGCCCAC
  GGGGCCGAGGTCAACTGGGCGGACGCGGAGGATGAGGGCAAGACGCCGCTGGTGCAGGCCGTGCTAGGG
  GGCTCCTTGATCGTCTGTGAGTTCCTGCTGCAAAACGGAGCGGACGTGAACCAAAGAGACAGCCGGGGC
  CGGGCGCCCCTGCACCACGCCACGCTGCTGGGCCGCACCGGCCAGGTTTGCCTGTTCCTGAAGCGGGGC
  GCGGACCAGCACGCCCTGGACCAAGAGCAGCGGGACCCGTTGGCCATCGCAGTGCAGGCGGCCAACGCT
  GACATCGTGACACTGCTCCGTCTGGCGCGCATGGCGGAGGAAATGCGCGAGGCCGAGGCTGCCCCTGGT
  CCCCCGGGCGCCCTGGCGGGCAGCCCCACGGAGCTCCAGTTCCGCAGGTGTATCCAGGAGTTCATCAGC
  CTCCACCTGGAAGAGAGCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_030649
• Insert Size: 2505 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030649.2
• RefSeq Size: 3801 bp
• RefSeq ORF: 2505 bp
• Locus ID: 116983
• UniProt ID: Q96P50
• Cytogenetics: 1p36.33
• Domains: ArfGap, PH, ANK
• Protein Pathways: Endocytosis
• MW: 92.5 kDa
• Gene Summary: GTPase-activating protein for the ADP ribosylation factor family.[UniProtKB/Swiss-Prot Function]