SMURF1 (NM_020429) Human Untagged Clone

CAT#: SC309681

SMURF1 (untagged)-Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMURF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_020429 edited
  ATGTCGAACCCCGGGACACGCAGGAACGGCTCCAGCATCAAGATCCGTCTGACAGTGTTA
  TGTGCCAAGAACCTTGCAAAGAAAGACTTCTTCAGGCTCCCTGACCCTTTTGCAAAGATT
  GTCGTGGATGGGTCTGGGCAGTGCCACTCAACCGACACTGTGAAAAACACATTGGACCCA
  AAGTGGAACCAGCACTATGATCTATATGTTGGGAAAACGGATTCGATAACCATTAGCGTG
  TGGAACCATAAGAAAATTCACAAGAAACAGGGAGCTGGCTTCCTGGGCTGTGTGCGGCTG
  CTCTCCAATGCCATCAGCAGATTAAAAGATACCGGATACCAGCGTTTGGATCTATGCAAA
  CTAAACCCCTCAGATACTGATGCAGTTCGTGGCCAGATAGTGGTCAGTTTACAGACACGA
  GACAGAATAGGAACCGGCGGCTCGGTGGTGGACTGCAGAGGACTGTTAGAAAATGAAGGA
  ACGGTGTATGAAGACTCGGGGCCTGGGAGGCCGCTCAGCTGCTTCATGGAGGAACCAGCC
  CCTTACACAGATAGCACCGGTGCTGCTGCTGGAGGAGGGAATTGCAGGTTCGTGGAGTCC
  CCAAGTCAAGATCAAAGACTTCAGGCACAGCGGCTTCGAAACCCTGATGTGCGAGGTTCA
  CTACAGACGCCCCAGAACCGACCACACGGCCACCAGTCCCCGGAACTGCCCGAAGGCTAC
  GAACAAAGAACAACAGTCCAGGGCCAAGTTTACTTTTTGCATACACAGACTGGAGTTAGC
  ACGTGGCACGACCCCAGGATACCAAGTCCCTCGGGGACCATTCCTGGGGGAGATGCAGCT
  TTTCTATACGAATTCCTTCTACAAGGCCATACATCTGAGCCCAGAGACCTTAACAGTGTG
  AACTGTGATGAACTTGGACCACTGCCGCCAGGCTGGGAAGTCAGAAGTACAGTTTCTGGG
  AGGATATATTTTGTAGATCATAATAACCGAACAACCCAGTTTACAGACCCAAGGTTACAC
  CACATCATGAATCACCAGTGCCAACTCAAGGAGCCCAGCCAGCCGCTGCCACTGCCCAGT
  GAGGGCTCTCTGGAGGACGAGGAGCTTCCTGCCCAGAGATACGAAAGAGATCTAGTCCAG
  AAGCTGAAAGTCCTCAGACACGAACTGTCGCTTCAGCAGCCCCAAGCTGGTCATTGCCGC
  ATCGAAGTGTCCAGAGAAGAAATCTTTGAGGAGTCTTACCGCCAGATAATGAAGATGCGA
  CCGAAAGACTTGAAAAAACGGCTGATGGTGAAATTCCGTGGGGAAGAAGGTTTGGATTAC
  GGTGGTGTGGCCAGGGAGTGGCTTTACTTGCTGTGCCATGAAATGCTGAATCCTTATTAC
  GGGCTCTTCCAGTATTCTACGGACAATATTTACATGTTGCAAATAAATCCGGATTCTTCA
  ATCAACCCCGACCACTTGTCTTATTTCCACTTTGTGGGGCGGATCATGGGGCTGGCTGTG
  TTCCATGGACACTACATCAACGGGGGCTTCACAGTGCCCTTCTACAAGCAGCTGCTGGGG
  AAGCCCATCCAGCTCTCAGATCTGGAATCTGTGGACCCAGAGCTGCATAAGAGCTTGGTG
  TGGATCCTAGAGAACGACATCACGCCTGTACTGGACCACACCTTCTGCGTGGAACACAAC
  GCCTTCGGGCGGATCCTGCAGCATGAACTGAAACCCAATGGCAGAAATGTGCCAGTCACA
  GAGGAGAATAAGAAAGAATACGTCCGGTTGTATGTAAACTGGAGGTTTATGAGAGGAATC
  GAAGCCCAGTTCTTAGCTCTGCAGAAGGGGTTCAATGAGCTCATCCCTCAACATCTGCTG
  AAGCCTTTTGACCAGAAGGAACTGGAGCTGATCATAGGCGGCCTGGATAAAATAGACTTG
  AACGACTGGAAGTCGAACACGCGGCTGAAGCACTGTGTGGCCGACAGCAACATCGTGCGG
  TGGTTCTGGCAAGCGGTGGAGACGTTCGATGAAGAAAGGAGGGCCAGGCTCCTGCAGTTT
  GTGACTGGGTCCACGCGAGTCCCGCTCCAAGGCTTCAAGGCTTTGCAAGGTTCTACAGGC
  GCGGCAGGGCCCCGGCTGTTCACCATCCACCTGATAGACGCGAACACAGACAACCTTCCG
  AAGGCCCATACCTGCTTTAACCGGATCGACATTCCACCATATGAGTCCTATGAGAAGCTC
  TACGAGAAGCTGCTGACAGCCGTGGAGGAGACCTGCGGGTTTGCTGTGGAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020429 unedited
  GTTGCATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGCTGGATCCG
  CGGCTGCCCGATCGTTGGCGGGAGATGTCGAACCCCGGGACACGCAGGAACGGCTCCAGC
  ATCAAGATCCGTCTGACAGTGTTATGTGCCAAGAACCTTGCAAAGAAAGACTTCTTCAGG
  CTCCCTGACCCTTTTGCAAAGATTGTCGTGGATGGGTCTGGGCAGTGCCACTCAACCGAC
  ACTGTGAAAAACACATTGGACCCAAAGTGGAACCAGCACTATGATCTATATGTTGGGAAA
  ACGGATTCGATAACCATTAGCGTGTGGAACCATAAGAAAATTCACAAGAAACAGGGAGCT
  GGCTTCCTGGGCTGTGTGCGGCTGCTCTCCAATGCCATCAGCAGATTAAAAGATACCGGA
  TACCAGCGTTTGGATCTATGCAAACTAAACCCCTCAGATACTGATGCAGTTCGTGGCCAG
  ATAGTGGTCAGTTTACAGACACGAGACAGAATAGGAACCGGCGGCTCGGTGGTGGACTGC
  AGAGGACTGTTAGAAAATGAAGGAACGGTGTATGAAGACTCGGGGCCTGGGAGGCCGCTC
  AGCTGCTTCATGGAGGAACCAGCCCCTTACACAGATAGCACCGGTGCTGCTGCTGGAGGA
  GGGAATTGCAGGTTCGTGGAGTCCCCAAGTCAAGATCAAAGACTTCAGGCACAGCGGCTT
  CGAAACCCTGATGTGCGAGGTTCACTACAGACGCCCCAAAACCCGACCACACGGCCACCA
  GTCCCCGGGACTGCCCCGAAGGCTACGAACAAAGAACAACAGTCCAGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_020429 unedited
  ATAGGAGATGGCAATTCCAGGCCAGNAAAGCACTGGGGAGGGTCACAGGTGCCCCCGGGA
  TCTGTTAGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT
  TTAAATGTAAAGTCGTGAGTTTATTGCATATGTAACAAAATGAACCTGACCTCCTGGGCC
  CAGCCTGCTGTACAATCACTGTTTGTTTTGTGTTTCCAGCTGGTTCCATAACCACATTAA
  ATAGAACTAGTATTTCATTAAATACTTTTGATTTTGACATAGAACATTAGTGTACAACTT
  TCACAAAATAAATCAGTGATAAAAACAGTGGGAAGGATAACAAGGATAGCAGCAATACTT
  CAAAACAAGACATTACAAAATAAATTAAAAAATACGTTATAAAGTGGTTGAGAAACAAAA
  ATAAACACATTTTTAAAATCCATACTATGTTTCGGGAANGCTGCCGTGTGGCACACACCT
  TGCTGCAAATGGCGGGTGGGGGGACATTTTCTCTGGAAAAAGCCTTTTCCTGAATTTTCG
  GGTTTGGTGAACCCCTTTAATCTGAAATTGGGACCATACGTCCGAAACAATCTTAGGGGG
  TTCCAAATTTCTTTTTGGTTGGGCAAAATTTTTTTTTTAAAAAATTTGAAAAAAACCCCT
  TTTTTCGGGGCCCCCCCGGGCCTTTCCAGGGGAGCCTCAAATTTTTTTCTAAATTTAAAA
  AAACTCTTGGGGGAAGAGGGGGGTTTAACAAACACCCCCCCCGGAGGATGGCGGGGCG
  
• Restriction Sites: Please inquire
• ACCN: NM_020429
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020429.1, NP_065162.1
• RefSeq Size: 5737 bp
• RefSeq ORF: 2274 bp
• Locus ID: 57154
• UniProt ID: Q9HCE7
• Cytogenetics: 7q22.1
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (1) encodes the longer isoform (1) of this protein.