STAT3 (NM_003150) Human Untagged Clone

CAT#: SC309567

STAT3 (untagged)-Human signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAT3
• Synonyms: ADMIO; ADMIO1; APRF; HIES
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003150 edited
  GGATCCTGGACAGGCACCCCGGCTTGGCGCTGTCTCTCCCCCTCGGCTCGGAGAGGCCCT
  TCGGCCTGAGGGAGCCTCGCCGCCCGTCCCCGGCACACGCGCAGCCCCGGCCTCTCGGCC
  TCTGCCGGAGAAACAGGATGGCCCAATGGAATCAGCTACAGCAGCTTGACACACGGTACC
  TGGAGCAGCTCCATCAGCTCTACAGTGACAGCTTCCCAATGGAGCTGCGGCAGTTTCTGG
  CCCCTTGGATTGAGAGTCAAGATTGGGCATATGCGGCCAGCAAAGAATCACATGCCACTT
  TGGTGTTTCATAATCTCCTGGGAGAGATTGACCAGCAGTATAGCCGCTTCCTGCAAGAGT
  CGAATGTTCTCTATCAGCACAATCTACGAAGAATCAAGCAGTTTCTTCAGAGCAGGTATC
  TTGAGAAGCCAATGGAGATTGCCCGGATTGTGGCCCGGTGCCTGTGGGAAGAATCACGCC
  TTCTACAGACTGCAGCCACTGCGGCCCAGCAAGGGGGCCAGGCCAACCACCCCACAGCAG
  CCGTGGTGACGGAGAAGCAGCAGATGCTGGAGCAGCACCTTCAGGATGTCCGGAAGAGAG
  TGCAGGATCTAGAACAGAAAATGAAAGTGGTAGAGAATCTCCAGGATGACTTTGATTTCA
  ACTATAAAACCCTCAAGAGTCAAGGAGACATGCAAGATCTGAATGGAAACAACCAGTCAG
  TGACCAGGCAGAAGATGCAGCAGCTGGAACAGATGCTCACTGCGCTGGACCAGATGCGGA
  GAAGCATCGTGAGTGAGCTGGCGGGGCTTTTGTCAGCGATGGAGTACGTGCAGAAAACTC
  TCACGGACGAGGAGCTGGCTGACTGGAAGAGGCGGCAACAGATTGCCTGCATTGGAGGCC
  CGCCCAACATCTGCCTAGATCGGCTAGAAAACTGGATAACGTCATTAGCAGAATCTCAAC
  TTCAGACCCGTCAACAAATTAAGAAACTGGAGGAGTTGCAGCAAAAAGTTTCCTACAAAG
  GGGACCCCATTGTACAGCACCGGCCGATGCTGGAGGAGAGAATCGTGGAGCTGTTTAGAA
  ACTTAATGAAAAGTGCCTTTGTGGTGGAGCGGCAGCCCTGCATGCCCATGCATCCTGACC
  GGCCCCTCGTCATCAAGACCGGCGTCCAGTTCACTACTAAAGTCAGGTTGCTGGTCAAAT
  TCCCTGAGTTGAATTATCAGCTTAAAATTAAAGTGTGCATTGACAAAGACTCTGGGGACG
  TTGCAGCTCTCAGAGGATCCCGGAAATTTAACATTCTGGGCACAAACACAAAAGTGATGA
  ACATGGAAGAATCCAACAACGGCAGCCTCTCTGCAGAATTCAAACACTTGACCCTGAGGG
  AGCAGAGATGTGGGAATGGGGGCCGAGCCAATTGTGATGCTTCCCTGATTGTGACTGAGG
  AGCTGCACCTGATCACCTTTGAGACCGAGGTGTATCACCAAGGCCTCAAGATTGACCTAG
  AGACCCACTCCTTGCCAGTTGTGGTGATCTCCAACATCTGTCAGATGCCAAATGCCTGGG
  CGTCCATCCTGTGGTACAACATGCTGACCAACAATCCCAAGAATGTAAACTTTTTTACCA
  AGCCCCCAATTGGAACCTGGGATCAAGTGGCCGAGGTCCTGAGCTGGCAGTTCTCCTCCA
  CCACCAAGCGAGGACTGAGCATCGAGCAGCTGACTACACTGGCAGAGAAACTCTTGGGAC
  CTGGTGTGAATTATTCAGGGTGTCAGATCACATGGGCTAAATTTTGCAAAGAAAACATGG
  CTGGCAAGGGCTTCTCCTTCTGGGTCTGGCTGGACAATATCATTGACCTTGTGAAAAAGT
  ACATCCTGGCCCTTTGGAACGAAGGGTACATCATGGGCTTTATCAGTAAGGAGCGGGAGC
  GGGCCATCTTGAGCACTAAGCCTCCAGGCACCTTCCTGCTAAGATTCAGTGAAAGCAGCA
  AAGAAGGAGGCGTCACTTTCACTTGGGTGGAGAAGGACATCAGCGGTAAGACCCAGATCC
  AGTCCGTGGAACCATACACAAAGCAGCAGCTGAACAACATGTCATTTGCTGAAATCATCA
  TGGGCTATAAGATCATGGATGCTACCAATATCCTGGTGTCTCCACTGGTCTATCTCTATC
  CTGACATTCCCAAGGAGGAGGCATTCGGAAAGTATTGTCGGCCAGAGAGCCAGGAGCATC
  CTGAAGCTGACCCAGGCGCTGCCCCATACCTGAAGACCAAGTTTATCTGTGTGACACCAA
  CGACCTGCAGCAATACCATTGACCTGCCGATGTCCCCCCGCACTTTAGATTCATTGATGC
  AGTTTGGAAATAATGGTGAAGGTGCTGAACCCTCAGCAGGAGGGCAGTTTGAGTCCCTCA
  CCTTTGACATGGAGTTGACCTCGGAGTGCGCTACCTCCCCCATGTGAGGAGCTGAGAACG
  GAAGCTGCAGAAAGATACGACTGAGGCGCCTACCTGCATTCTGCCACCCCTCACACAGCC
  AAACCCCAGATCATCTGAAACTACTAACTTTGTGGTTCCAGATTTTTTTTAATCTCCTAC
  TTCTGCTATCTTTGAGCAATCTGGGCACTTTTAAAAATAGAGAAATGAGTGAAAAAAAAA
  AAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_003150
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003150.3, NP_003141.2
• RefSeq Size: 4953 bp
• RefSeq ORF: 2310 bp
• Locus ID: 6774
• UniProt ID: P40763
• Cytogenetics: 17q21.2
• Domains: SH2, STAT
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer
• Gene Summary: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.