GPR120 (FFAR4) (NM_181745) Human Untagged Clone

CAT#: SC307360

FFAR4 (untagged)-Human omega-3 fatty acid receptor 1 (O3FAR1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR120
• Synonyms: BMIQ10; GPR120; GPR129; GT01; O3FAR1; PGR4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC307360 representing NM_181745.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  CGCCCTTCACCATGTCCCCTGAATGCGCGCGGGCAGCGGGCGACGCGCCCTTGCGCAGCCTGGAACAAG
  CCAACCGCACCCGCTTTCCCTTCTTCTCCGACGTCAAGGGCGACCACCGGCTGGTGCTGGCCGCGGTGG
  AGACAACCGTGCTGGTGCTCATCTTTGCAGTGTCGCTGCTGGGCAACGTGTGCGCCCTGGTGCTGGTGG
  CGCGCCGACGACGCCGCGGCGCGACTGCCTGCCTGGTACTCAACCTCTTCTGCGCGGACCTGCTCTTCA
  TCAGCGCTATCCCTCTGGTGCTGGCCGTGCGCTGGACTGAGGCCTGGCTGCTGGGCCCCGTTGCCTGCC
  ACCTGCTCTTCTACGTGATGACCCTGAGCGGCAGCGTCACCATCCTCACGCTGGCCGCGGTCAGCCTGG
  AGCGCATGGTGTGCATCGTGCACCTGCAGCGCGGCGTGCGGGGTCCTGGGCGGCGGGCGCGGGCAGTGC
  TGCTGGCGCTCATCTGGGGCTATTCGGCGGTCGCCGCTCTGCCTCTCTGCGTCTTCTTCCGAGTCGTCC
  CGCAACGGCTCCCCGGCGCCGACCAGGAAATTTCGATTTGCACACTGATTTGGCCCACCATTCCTGGAG
  AGATCTCGTGGGATGTCTCTTTTGTTACTTTGAACTTCTTGGTGCCAGGACTGGTCATTGTGATCAGTT
  ACTCCAAAATTTTACAGACCTCGGAACACCTCCTGGATGCAAGAGCTGTCGTGACTCACAGTGAGATCA
  CAAAGGCATCAAGGAAGAGGCTCACGGTAAGCCTGGCCTACTCGGAGAGCCACCAGATCCGCGTGTCCC
  AGCAGGACTTCCGGCTCTTCCGCACCCTCTTCCTCCTCATGGTCTCCTTCTTCATCATGTGGAGCCCCA
  TCATCATCACCATCCTCCTCATCCTGATCCAGAACTTCAAGCAAGACCTGGTCATCTGGCCGTCCCTCT
  TCTTCTGGGTGGTGGCCTTCACATTTGCTAATTCAGCCCTAAACCCCATCCTCTACAACATGACACTGT
  GCAGGAATGAGTGGAAGAAAATTTTTTGCTGCTTCTGGTTCCCAGAAAAGGGAGCCATTTTAACAGACA
  CATCTGTCAAAAGAAATGACTTGTCGATTATTTCTGGCTAATGTTTTCAAAGGGCGAATTCAGATCTGG
  TACCGATATCAAGCTTGTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181745 unedited
  GGAGTTCAAAATTGTATACGACTCACTATAGGGCGGCCGCGAATCGCCCTTCACCATGTC
  CCCTGAATGCGCGCGGGCAGCGGGCGACGCGCCCTTGCGCAGCCTGGAACAAGCCAACCG
  CACCCGCTTTCCCTTCTTCTCCGACGTCAAGGGCGACCACCGGCTGGTGCTGGCCGCGGT
  GGAGACAACCGTGCTGGTGCTCATCTTTGCAGTGTCGCTGCTGGGCAACGTGTGCGCCCT
  GGTGCTGGTGGCGCGCCGACGACGCCGCGGCGCGACTGCCTGCCTGGTACTCAACCTCTT
  CTGCGCGGACCTGCTCTTCATCAGCGCTATCCCTCTGGTGCTGGCCGTGCGCTGGACTGA
  GGCCTGGCTGCTGGGCCCCGTTGCCTGCCACCTGCTCTTCTACGTGATGACCCTGAGCGG
  CAGCGTCACCATCCTCACGCTGGCCGCGGTCAGCCTGGAGCGCATGGTGTGCATCGTGCA
  CCTGCAGCGCGGCGTGCGGGGTCCTGGGCGGCGGGCGCGGGCAGTGCTGCTGGCGCTCAT
  CTGGGGCTATTCGGCGGTCGCCGCTCTGCCTCTCTGCGTCTTCTTCCGAGTCGTCCCGCA
  ACGGCTCCCCGGCGCCGACCAGGAAATTTCGATTTGCACACTGATTTGGCCCACCATTCC
  TGGAGAGATCTCGTGGGATGTCTCTTTTGTTACTTTGAACTTCTTGGTGCCAGGACTGGT
  CATTGTGATCAGTTACTCCAAAATTTTACAGACCTCGGGACACCTCCTGGATGCAAGAGC
  TGTCGTGACTCACAGTGAGATCACCAAAGCATCAAGGAAGAAGCTCACGGTAAGCCTGGC
  CTACTCGGAGAGCCACAGATCCGCGTGTCCCAGCAGGACTTTCGGCTCTTCCGCACCCTC
  T
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_181745 unedited
  AGNAAAGCACTGGGGNAGGGTCACAGGNATGCCACCCGGGCTCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGACAAGCTTGATATCGGTACCAGATCTGAATTCGCC
  CTTTGAAAACATTAGCCAGAAATAATCGACAAGTCATTTCTTTTGACAGATGTGTCTGTT
  AAAATGGCTCCCTTTTCTGGGAACCAGAAGCAGCAAAAAATTTTCTTCCACTCATTCCTG
  CACAGTGTCATGTTGTAGAGGATGGGGTTTAGGGCTGAATTAGCAAATGTGAAGGCCACC
  ACCCAGAAGAAGAGGGACGGCCAGATGACCAGGTCTTGCTTGAAGTTCTGGATCAGGATG
  AGGAGGATGGTGATGATGATGGGGCTCCACATGATGAAGAAGGAGACCATGAGGAGGAAG
  AGGGTGCGGAAGAGCCGGAAGTCCTGCTGGGACACGCGGATCTGGTGGCTCTCCGAGTAG
  GCCAGGCTTACCGTGAGCCTCTTCCTTGATGCCTTTGTGATCTCACTGTGAGTCACGACA
  GCTCTTGCATCCAGGAGGTGTTCCGAGGTCTGTAAAATTTTGGAGTAACTGATCACAATG
  ACCAGTCCTGGCACCAAGAAGTTCAAAGTAACAAAAGAGACATCCCACGAGATCTCTCCA
  GGAATGGTGGGCCAAATCAGTGTGCAAATCGAAATTTCCTGGTCGGCGCCGGNGAGCCGT
  TGCGGGACGACTCGGAAGAAGACGCAGAGAGGCAGAGCGGCGACCGCCGAATAGCCCCAG
  ATGAGCGCCAGCAGCACTGCCCGCGCCCGCCGCCCAGACCCCGCACGCCGGGCTGGCAGT
  GCACGATGCACACCATGCGCTNCAGGCTGACG
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_181745
• Insert Size: 1233 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181745.2
• RefSeq Size: 1190 bp
• RefSeq ORF: 1233 bp
• Locus ID: 338557
• UniProt ID: Q5NUL3
• Cytogenetics: 10q23.33
• Protein Families: Druggable Genome, Transmembrane
• MW: 45 kDa
• Gene Summary: This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (1) encodes the longer isoform (GPR120-L). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.