OTOP3 (NM_178233) Human Untagged Clone

CAT#: SC307144

OTOP3 (untagged)-Human otopetrin 3 (OTOP3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OTOP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC307144 representing NM_178233.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGCCGCGGAGCCCGAGCGGCGGCTGCGCAGTCCCGCTGGGGGAGGGCGTCGCGGGCATCGGTCTCA
  CCTGGACGGACGATCCGCTCAGCGCCCGCAGTCGGTGAAGCACAGGAGACTGAAGCAGCCCCGGAGAAG
  GAGAACCGAGTGGATGTGGGGGCCGAGGAGAGAGCGGCCGCCACCCGGCCCCGGCAGAAGTCCTGGCTG
  GTGAGGCATTTCTCTCTGCTGCTGCGGCGGGACCGGCAGGCCCAGAAGGCTGGACAACTCTTCTCGGGG
  CTCCTGGCCCTGAATGTGGTGTTCCTGGGTGGCGCCTTCATCTGCAGCATGATCTTCAACAAGGTGGCC
  GTCACTCTGGGTGACGTGTGGATCCTGCTGGCCACGCTGAAGGTCCTCTCCCTGCTTTGGCTTCTCTAC
  TATGTGGCAAGCACCACCCGCCGACCACACGCCGTGCTCTACCAAGATCCCCACGCGGGGCCCCTCTGG
  GTGCGGGGTTCCCTAGTGCTCTTCGGCAGCTGCACCTTCTGCCTCAACATCTTCCGAGTGGGCTACGAT
  GTGAGCCACATCCGCTGCAAGTCACAGCTGGACCTTGTCTTCTCTGTCATCGAGATGGTCTTCATCGGC
  GTCCAGACCTGGGTGCTCTGGAAACACTGCAAAGACTGTGTTCGGGTCCAGACCAACTTCACTAGGTGT
  GGCCTGATGCTGACCCTGGCCACAAACCTGCTGCTGTGGGTTCTGGCCGTTACCAATGACTCCATGCAC
  CGAGAGATCGAAGCTGAGCTTGGCATCCTCATGGAAAAATCCACAGGCAATGAGACCAACACCTGTCTG
  TGCCTCAATGCCACCGCGTGTGAAGCTTTCCGGAGAGGCTTCCTGATGCTCTACCCCTTCAGCACTGAG
  TACTGCCTCATCTGCTGTGCTGTGCTGTTTGTCATGTGGAAGAACGTGGGCCGCCACGTGGCACCCCAC
  ATGGGTGCCCACCCTGCCACCGCACCCTTCCACCTGCACGGGGCCATCTTCGGGCCGCTGCTGGGCCTG
  CTGGTGCTGCTGGCAGGTGTGTGCGTCTTTGTGCTCTTCCAAATCGAGGCCAGTGGCCCTGCCATTGCT
  TGCCAGTACTTCACCCTCTACTATGCCTTCTATGTGGCTGTGCTGCCCACCATGAGTCTGGCGTGCCTG
  GCGGGCACAGCCATACACGGGCTGGAGGAGAGAGAGCTGGACACGGTCAAGAACCCTACCCGCAGCCTG
  GATGTGGTGCTGCTAATGGGTGCTGCACTGGGCCAGATGGGCATCGCCTATTTCTCCATCGTGGCCATT
  GTGGCCAAGCGCCCGCATGAGCTGCTCAACCGCCTCATCCTGGCCTACTCGCTGCTGCTCATCCTGCAG
  CACATCGCTCAGAACCTCTTCATCATCGAGGGCCTGCACCGGCGCCCACTCTGGGAGACAGTTCCCGAG
  GGCCTGGCAGGAAAGCAGGAGGCTGAGCCTCCCCGCAGAGGCTCCTTGCTGGAGCTGGGCCAGGGCCTG
  CAGCGGGCCTCACTGGCCTACATCCACTCCTACAGCCACCTCAACTGGAAGCGGAGGGCACTCAAGGAG
  ATCTCACTCTTCCTCATCCTCTGCAATATCACACTGTGGATGATGCCTGCATTTGGCATACACCCGGAG
  TTTGAGAACGGGCTAGAAAAGGATTTCTACGGCTACCAGATATGGTTCGCCATCGTCAACTTCGGCCTG
  CCTCTGGGGGTCTTCTACCGCATGCACTCTGTGGGAGGCCTGGTGGAGGTCTACCTGGGGGCCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_178233
• Insert Size: 1791 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178233.2
• RefSeq Size: 2367 bp
• RefSeq ORF: 1791 bp
• Locus ID: 347741
• UniProt ID: Q7RTS5
• Cytogenetics: 17q25.1
• Protein Families: Transmembrane
• MW: 66.3 kDa
• Gene Summary: Proton-selective channel that specifically transports protons into cells. Proton-selective channel activity is probably required in cell types that use changes in intracellular pH for cell signaling or to regulate biochemical or developmental processes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.