GLIS3 (NM_152629) Human Untagged Clone

CAT#: SC306467

GLIS3 (untagged)-Human GLIS family zinc finger 3 (GLIS3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLIS3
• Synonyms: NDH; ZNF515
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_152629 edited
  ATCCTTTATGGTTGAAGGGTGTTGGAGGAAGCAGAGTTTTCATCAGCTGCTGAAGTGGCA
  ACTAATAGAGGTTTGAATGGCCGTAGAAAAATATTGTGTTACTTGTTTGTTGTCTAGGGG
  CTCTTGGCTTTGGGCCTCAGTGCAAGTCCATTGGAAAAGGCAGCTGCAACAATCTAGTGG
  TCACCAGCAGTCCCATGATGGTTCAGCGACTGGGACTCATTTCACCTCCAGCAAGCCAGG
  TCTCTACAGCATGCAACCAGATCAGTCCTAGCTTACAGAGGGCAATGAATGCAGCCAACC
  TGAATATACCTCCTTCAGATACCAGGTCCCTTATTTCGCGTGAGTCTTTGGCGTCCACGA
  CCTTGAGTCTGACGGAAAGTCAGTCGGCCTCAAGCATGAAGCAGGAGTGGTCCCAGGGCT
  ACAGGGCCCTCCCTTCGCTCTCCAACCACGGCTCTCAGAATGGCCTTGATCTAGGGGATC
  TCCTTAGCCTTCCTCCCGGGACATCCATGTCCAGCAATAGTGTCTCTAACTCATTACCAT
  CCTACCTTTTTGGCACGGAAAGTAGCCACTCTCCTTACCCTAGTCCTCGGCACTCATCCA
  CCAGGTCCCACTCGGCCCGCTCCAAGAAGAGAGCGCTGTCCTTGTCCCCGCTGTCCGATG
  GCATCGGGATAGATTTCAATACCATCATCCGCACGTCGCCCACGTCCTTGGTGGCCTACA
  TCAACGGGTCGAGGGCTTCGCCGGCCAACCTGTCCCCGCAGCCGGAGGTCTACGGGCATT
  TCCTGGGCGTGCGCGGCAGCTGCATTCCCCAGCCGCGCCCGGTGCCCGGCAGCCAGAAGG
  GCGTGCTGGTGGCCCCTGGAGGCCTGGCGCTGCCGGCCTACGGCGAGGACGGGGCCCTGG
  AGCACGAGCGCATGCAACAGCTGGAGCACGGCGGCCTGCAGCCAGGCCTGGTCAACCACA
  TGGTGGTGCAGCATGGCCTGCCGGGCCCCGACAGCCAGCCGGCCGGCCTGTTCAAGACCG
  AACGCCTGGAGGAGTTCCCGGGCAGCACCGTAGACCTACCCCCCGCGCCTCCGCTCCCTC
  CTCTGCCGCCGCCCCAAGGCCCCCCACCCCCTTACCATGCCCATGCGCACCTTCACCACC
  CGGAGCTCGGGCCCCACGCCCAGCAGCTGGCCTTGCCCCAGGCCACCCTGGACGACGACG
  GGGAGATGGACGGCATCGGGGGCAAGCATTGCTACCGCTGGATCGACTGCAGCGCCCTGT
  ACGACCAGCAGGAGGAGCTCGTGCGGCACATCGAGAAGGTCCACATCGACCAGCGCAAAG
  GGGAGGACTTCACTTGCTTCTGGGCCGGTTGCCCTCGAAGATACAAGCCCTTCAACGCCC
  GCTATAAACTGCTGATCCACATGAGAGTCCACTCTGGGGAGAAGCCCAACAAGTGTACGT
  TTGAAGGTTGCGAGAAGGCCTTTTCAAGGCTTGAAAATCTCAAGATCCACTTGCGGAGCC
  ACACAGGCGAGAAGCCGTATTTGTGCCAGCATCCGGGTTGTCAGAAGGCCTTCAGTAACT
  CCAGTGACCGCGCCAAACACCAGCGGACGCATCTGGACACCAAACCTTATGCTTGTCAAA
  TTCCAGGATGTACCAAACGCTACACAGACCCAAGTTCCCTAAGAAAGCATGTGAAGGCAC
  ATTCTTCCAAAGAGCAACAAGCAAGGAAAAAGTTGCGGTCCAGCACAGAGCTCCATCCAG
  ACCTGCTCACAGATTGCCTCACCGTGCAGTCCCTGCAGCCGGCCACTTCCCCTAGAGATG
  CTGCTGCTGAAGGGACCGTGGGACGCTCCCCTGGACCCGGGCCTGACCTCTATTCAGCTC
  CCATTTTCTCCAGCAATTATTCAAGCCGAAGTGGAACAGCTGCTGGGGCCGTACCACCCC
  CACATCCTGTCAGTCACCCTTCTCCAGGACATAATGTACAGGGGAGCCCTCACAACCCCT
  CCTCCCAGTTACCTCCACTCACAGCTGTGGACGCAGGAGCTGAGAGGTTTGCACCTTCTG
  CTCCATCTCCTCACCACATCAGCCCCCGGAGAGTTCCAGCTCCTTCTTCAATACTGCAAA
  GAACACAGCCTCCCTATACCCAGCAGCCATCAGGTTCACACCTGAAGTCCTATCAGCCAG
  AAACAAACTCTTCTTTTCAACCAAATGGTATCCATGTCCATGGATTTTATGGGCAGCTGC
  AGAAGTTCTGTCCCCCACACTACCCCGATTCCCAGAGAATTGTGCCGCCTGTCAGCTCCT
  GCAGTGTGGTGCCTTCGTTTGAGGACTGCCTAGTCCCTACATCCATGGGCCAGGCCAGTT
  TTGATGTTTTCCACAGAGCCTTCTCGACTCACTCGGGCATTACAGTGTATGATTTACCTT
  CAAGTTCCTCGAGCCTCTTTGGGGAGTCTCTCCGCAGCGGGGCTGAAGATGCTACCTTCT
  TGCAGATCAGCACCGTGGACCGCTGTCCTAGCCAGCTCTCCTCTGTCTACACCGAAGGCT
  AAAAGCTCTCTTGGCCACTCCTGCACACCCAGGACCTTGATGTTGCTTGCCACCTTTTGT
  TTTCGTACTCTCACAGACTGCTTGAGGAAGGATGTCAGCCCAATCAGCGGGCCCTGCAGA
  GTTACTGATGTTCTTTCAGAAGGCAGGACTGAATGGCAGAGCTAGCTTTGCAGGAATCAT
  TACAGTTGCTCCTTTTTCTCTCTGGTTTTCCTGGACTATGTGACCAGGAGGACTGTCTTT
  TCAAAGGGAATTTAGAGCCTCACTCTCCTGGATACCTGTTACTTCCTGGCTGTCAACCCT
  TAAAGGACCCCAGTGAAATGATGTGCATATGAAAATGTAGTTTGGGGATGACTCTACCTG
  AAAACTCCAAACAGGGAAAAAGACCTCAATTACGAGAGGCATAGTGACCTGCCTCACCTA
  ATCCTCACCCTTGCTGCTCCTGATGCAAAACCAGGCACAACAAGGGAAATCCATGTCCAA
  AGCATAGTGTGGTCTCAACTCTTAGAAACTCCCTGGGTTTCCCTTTTCTCTTAGCCACAT
  GCTAACCAGTTGCTTCTCAGTGTTTCTCTTCAATAGAAAAATAAGGTTTTATATGCCAAA
  AAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_152629
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_152629.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152629.2, NP_689842.2
• RefSeq Size: 3133 bp
• RefSeq ORF: 2328 bp
• Locus ID: 169792
• UniProt ID: Q8NEA6
• Cytogenetics: 9p24.2
• Gene Summary: This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (b) has a shorter N-terminus when compared to isoform 1.