PANK1 (NM_148977) Human Untagged Clone

CAT#: SC306349

PANK1 (untagged)-Human pantothenate kinase 1 (PANK1), transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PANK1
• Synonyms: PANK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC306349 representing NM_148977.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTGAAACTCGTCGGTGGCGGTGGCGGGCAGGACTGGGCATGCTCAGTGGCGGGGACCAGTCTGGGA
  GGCGAGGAAGCCGCGTTTGAAGTCGCGCGGCCTGGGGATCAGGGGAAGGCGGGCGGCGGGAGCCCCGGC
  TGGGGGTGCGCGGGGATCCCCGATTCCGCGCCCGGGGCTGGCGTGCTCCAGGCCGGCGCCGTGGGGCCG
  GCGCGCGGGGGGCAGGGCGCAGAGGAGGTGGGGGAGTCGGCAGGAGGAGGGGAGGAGCGCCGGGTTCGC
  CATCCCCAGGCGCCGGCTCTGCGGCTGCTGAATCGGAAGCCGCAGGGAGGATCCGGGGAAATAAAGACG
  CCGGAGAATGACCTCCAGCGAGGCCGCCTGAGCCGGGGCCCGCGCACAGCCCCGCCAGCCCCCGGCATG
  GGCGACCGCAGCGGGCAGCAGGAGCGCTCGGTCCCGCACTCTCCAGGGGCCCCCGTGGGCACCAGCGCC
  GCCGCTGTGAACGGGCTGCTGCACAACGGCTTCCATCCGCCGCCAGTCCAGCCGCCGCACGTCTGCAGC
  CGGGGTCCAGTGGGCGGCAGCGACGCGGCGCCCCAGCGCCTCCCGCTCCTGCCGGAGCTGCAGCCGCAG
  CCACTGCTCCCTCAGCATGACTCCCCGGCCAAGAAATGCCGGCTGCGGAGGAGGATGGACTCGGGGAGA
  AAGAACAGGCCGCCATTCCCATGGTTTGGCATGGACATCGGTGGAACGCTGGTTAAATTGGTGTATTTC
  GAGCCGAAGGATATTACAGCCGAAGAGGAGCAAGAGGAAGTGGAGAACCTGAAGAGCATCCGGAAGTAT
  TTGACTTCTAATACTGCTTATGGGAAAACTGGGATCCGAGACGTCCACCTGGAACTGAAAAACCTGACC
  ATGTGTGGACGCAAAGGGAACCTGCACTTCATCCGCTTTCCCAGCTGTGCTATGCACAGGTTCATTCAG
  ATGGGCAGCGAGAAGAACTTCTCTAGCCTTCACACCACCCTCTGTGCCACAGGAGGCGGGGCTTTCAAA
  TTCGAAGAGGACTTCAGAATGATTGCTGACCTGCAGCTGCATAAACTGGATGAACTGGACTGTCTGATT
  CAGGGCCTGCTTTATGTCGACTCTGTTGGCTTCAACGGCAAGCCAGAATGTTACTATTTTGAAAATCCC
  ACAAATCCTGAATTGTGTCAAAAAAAGCCGTACTGCCTTGATAACCCATACCCTATGTTGCTGGTTAAC
  ATGGGCTCAGGTGTCAGCATTCTAGCCGTGTACTCCAAGGACAACTATAAAAGAGTTACAGGGACCAGT
  CTTGGAGGTGGAACATTCCTAGGCCTATGTTGCTTGCTGACTGGTTGTGAGACCTTTGAAGAAGCTCTG
  GAAATGGCAGCTAAAGGCGACAGCACCAATGTTGATAAACTGGTGAAGGACATTTACGGAGGAGACTAT
  GAACGATTTGGCCTTCAAGGATCTGCTGTAGCATCAAGCTTTGGCAACATGATGAGTAAAGAAAAGCGA
  GATTCCATCAGCAAGGAAGACCTCGCCCGGGCCACATTGGTCACCATCACCAACAACATTGGCTCCATT
  GCTCGGATGTGCGCGTTGAATGAGAACATAGACAGAGTTGTGTTTGTTGGAAATTTTCTCAGAATCAAT
  ATGGTCTCCATGAAGCTGCTGGCATATGCCATGGATTTTTGGTCCAAAGGACAACTGAAAGCTCTGTTT
  TTGGAACATGAGGGTTATTTTGGAGCCGTTGGGGCACTGTTGGAACTGTTCAAAATGACTGATGACAAG
  TAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_148977
• Insert Size: 1797 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148977.2
• RefSeq Size: 6976 bp
• RefSeq ORF: 1797 bp
• Locus ID: 53354
• UniProt ID: Q8TE04
• Cytogenetics: 10q23.31
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis
• MW: 64.3 kDa
• Gene Summary: This gene encodes a member of the pantothenate kinase family. Pantothenate kinases are key regulatory enzymes in the biosynthesis of coenzyme A (CoA). The encoded protein catalyzes the first and rate-limiting enzymatic reaction in CoA biosynthesis and is regulated by CoA through feedback inhibition. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene and an intronic miRNA on the same strand are co-regulated by the tumor suppressor p53 (see PMID 20833636). [provided by RefSeq, Apr 2011]
  Transcript Variant: This variant (alpha) represents the longest transcript and encodes the longest isoform (alpha). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.