Angiomotin (AMOT) (NM_133265) Human Untagged Clone

CAT#: SC305938

AMOT (untagged)-Human angiomotin (AMOT), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Angiomotin
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_133265 edited
  CTTGGTCCCAAACCAGGGAGACCATTCAGCTCACCTGCCTAGGCCGCAGCAGCATTTCCT
  TCCTAATCAGGCTCACCAGGGGGATCATTACCGTCTCTCCCAACCTGGCCTGAGTCAGCA
  GCAGCAGCAACAGCAGCAGCAGCACCATCATCACCATCACCACCAACAACAGCAGCAGCA
  GCAGCCACAGCAGCAGCCAGGAGAAGCCTATTCAGCTATGCCTCGGGCTCAGCCATCCTC
  TGCTTCTTATCAGCCAGTGCCAGCAGACCCTTTTGCCATTGTTTCCAGAGCCCAGCAGAT
  GGTTGAGATCCTCTCAGACGAGAACCGGAACTTGAGGCAAGAGTTGGAAGGATGCTATGA
  GAAGGTGGCAAGACTGCAGAAGGTGGAGACAGAAATCCAGCGCGTCTCGGAGGCATATGA
  GAACCTCGTGAAGTCATCCTCCAAAAGAGAGGCCCTAGAGAAAGCCATGAGAAACAAGCT
  AGAGGGCGAGATTCGGAGGATGCATGATTTCAACAGGGATCTGAGAGAGCGTCTAGAGAC
  TGCCAACAAGCAGCTTGCAGAGAAGGAATATGAGGGGTCAGAGGACACCAGAAAAACCAT
  CTCGCAGCTCTTTGCAAAAAATAAAGAAAGCCAGCGTGAGAAGGAGAAGCTGGAAGCGGA
  GCTGGCCACTGCCCGTTCTACCAATGAGGACCAAAGACGACACATCGAAATCCGAGATCA
  GGCCCTGAGTAATGCCCAGGCCAAGGTGGTAAAGCTGGAAGAAGAGCTGAAAAAGAAGCA
  AGTGTACGTTGACAAGGTGGAGAAGATGCAGCAGGCCCTTGTACAGCTCCAGGCAGCATG
  TGAAAAACGTGAGCAGCTAGAGCACCGTCTCCGGACACGACTGGAGAGGGAACTGGAATC
  CCTGAGAATCCAGCAGCGTCAGGGCAACTGTCAGCCCACCAACGTTTCAGAATACAATGC
  TGCTGCACTGATGGAGCTCCTTCGGGAGAAAGAGGAGAGGATTCTGGCTCTGGAAGCTGA
  TATGACAAAGTGGGAGCAGAAATATTTGGAGGAGAATGTGATGAGACATTTTGCTCTGGA
  TGCTGCTGCAACTGTGGCTGCTCAGAGGGACACAACAGTCATCAGTCACTCTCCTAACAC
  CAGCTATGACACAGCTCTAGAAGCTCGCATCCAGAAAGAGGAGGAAGAAATCTTGATGGC
  CAATAAGCGTTGCCTTGACATGGAGGGCAGGATTAAGACCCTCCATGCCCAGATTATTGA
  GAAGGATGCCATGATCAAAGTACTCCAGCAGCGTTCCCGGAAGGAGCCGAGCAAGACAGA
  GCAGCTGTCGTGCATGCGGCCAGCGAAGTCTCTGATGTCCATTTCCAATGCTGGATCAGG
  CTTGCTCTCCCACTCATCCACCCTGACTGGCTCCCCCATCATGGAAGAAAAGCGAGACGA
  CAAGAGCTGGAAGGGGAGCCTAGGCATTCTCCTGGGTGGAGACTACCGTGCTGAATATGT
  CCCTTCCACACCCTCGCCTGTGCCACCCTCGACTCCCCTGCTCTCGGCTCACTCCAAGAC
  AGGCAGCCGAGACTGCAGTACCCAAACTGAACGTGGGACGGAATCGAACAAAACTGCAGC
  TGTTGCTCCCATCTCTGTTCCTGCTCCAGTTGCTGCTGCCGCCACTGCTGCCGCCATCAC
  TGCCACTGCTGCCACCATCACCACCACCATGGTAGCTGCTGCTCCAGTTGCTGTTGCTGC
  TGCTGCTGCTCCAGCTGCTGCTGCTGCCCCGTCTCCAGCCACTGCCGCTGCTACTGCTGC
  TGCTGTTTCTCCAGCTGCTGCTGGTCAGATTCCAGCTGCTGCCTCTGTTGCCTCAGCTGC
  TGCCGTTGCTCCTTCTGCTGCTGCTGCTGCTGCTGTTCAGGTTGCTCCAGCTGCTCCGGC
  TCCAGTTCCAGCTCCGGCTCTGGTTCCGGTTCCAGCTCCAGCAGCGGCTCAGGCTTCTGC
  TCCTGCTCAGACTCAGGCACCAACTTCAGCTCCGGCTGTGGCTCCAACTCCAGCTCCAAC
  TCCAACTCCAGCTGTGGCTCAGGCTGAGGTTCCTGCAAGTCCAGCTACCGGTCCTGGACC
  ACATCGTTTGTCTATACCAAGTTTGACCTGCAATCCAGACAAAACAGATGGGCCTGTGTT
  CCACTCCAATACTCTGGAAAGAAAAACTCCCATTCAGATCCTGGGACAAGAGCCTGATGC
  AGAGATGGTGGAATATCTCATCTAAACGGCCAAATCAAGAGCTGCAGATTATCAGCAAAA
  ATGCTTTTAATCATTTTCCCCCTTTTATTGGTTCTTGTTTTGAGGGTGAGGACAAGGGTT
  GTGGGGAGGGGATGTTTTTTAACAGGACTTTTTATTGGAACAATGTACTACTTGAGTAAT
  ACCATGTGAACACCAGTCTATTTTGGTATGCTTAGGGAGTACCTCTAAAGACAGATTAAT
  CAGAATGTGCTCTAAAGCTTATTGTTTGAATTTATACGAATACTGGGACTGTTAACAGGT
  GGCTATACATCGACGTTTTCAATGTGCTTAAATTTGTTTAAATTTTCCATATTCTAGATC
  ATTTTTTATTGAAGAGCACAGTATGTGTGGAAGACAGTGTATAACACGTAGTTTGGAAGT
  GGGAAGCTAGAGAGAATTGAGTGTGTGCTGTTTTGTATAGTTACTATCCTGTGCAGCAGC
  TGGAGAAAGCACTCACCTCAGGCTTACAAAAGGGAATAGTTTCGGGAGCTATGTAAGCTG
  GAAAAAAGGTAGGGAGTTTTGGGGTGCAGAAGGGTACTGG
  
• Restriction Sites: Please inquire
• ACCN: NM_133265
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133265.2, NP_573572.1
• RefSeq Size: 6514 bp
• RefSeq ORF: 2028 bp
• Locus ID: 154796
• UniProt ID: Q4VCS5
• Cytogenetics: Xq23
• Gene Summary: This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. This difference results in translation initiation at a downstream ATG and an isoform (2) with a shorter N-terminus, compared to isoform 1. This isoform lacks the N-terminal extension present in isoform 1 that mediates association with cytoplasmic F-actin.