SPIRE2 (NM_032451) Human Untagged Clone

CAT#: SC305455

SPIRE2 (untagged)-Human spire homolog 2 (Drosophila) (SPIRE2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPIRE2
• Synonyms: Spir-2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032451 edited
  TCCGGCGCGCGGGAGGCGATGACGGCCCCGCCATGGCCCGGGCGGGCAGCTGCGGCGGCG
  CCGCGGCGGGCGCAGGGCGGCCGGAGCCCTGGGAGCTGTCCCTGGAGGAGGTGCTGAAGG
  CCTACGAGCAGCCGCTCAACGAGGAGCAGGCGTGGGCCGTGTGCTTCCAGGGCTGCCGCG
  GGCTGCGGGGCTCGCCGGGCCGGCGCCTGCGGGATACCGGGGACCTCCTGCTGCGCGGGG
  ACGGCTCGGTCGGGGCGCGGGAGCCCGAGGCCGCGGAACCTGCAACCATGGTCGTGCCAC
  TAGCCAGCTCGGAAGCCCAGACCGTGCAGTCCCTCGGCTTCGCCATCTACCGCGCGCTGG
  ACTGGGGGCTGGACGAGAGCGAGGAGCGCGAACTCAGCCCTCAGCTGGAGCGGCTCATCG
  ACCTCATGGCCAACAACGACAGCGAGGACAGCGGCTGCGGTGCCGCCGATGAGGGCTACG
  GGGGTCCCGAGGAGGAGGAGGAGGCCGAGGGCGTCCCCCGCAGCGTGCGCACCTTTGCCC
  AGGCCATGCGGCTGTGCGCGGCGCGGCTGACCGACCCCCGGGGCGCACAGGCGCATTACC
  AGGCCGTGTGCCGCGCGCTCTTCGTGGAGACGCTGGAGCTGCGGGCCTTCCTGGCCAGGG
  TCCGGGAGGCCAAGGAGATGCTGCAGAAGCTTCGGGAGGACGAGCCGCATCTGGAGACGC
  CTCGGGCAGAGCTGGACAGCCTGGGTCACACAGACTGGGCCCGACTGTGGGTTCAGCTCA
  TGCGGGAGCTCCGCCGCGGAGTGAAGCTGAAGAAGGTGCAAGAGCAGGAGTTCAACCCCC
  TCCCCACCGAGTTCCAGCTCACGCCCTTCGAGATGCTGATGCAGGACATCCGGGCCCGGA
  ACTACAAGCTGCGCAAGGTCATGGTGGATGGGGACATCCCGCCCCGGGTGAAGAAGGACG
  CTCACGAGCTCATCCTGGACTTTATCCGCTCACGGCCTCCACTGAAGCAGGTCTCTGAGA
  GGCGGCTGCGCCCGTTGCCACCAAAGCAAAGGTCCCTGCATGAGAAGATCCTGGAGGAGA
  TCAAGCAGGAGCGGAGGCTGCGCCCGGTGCGGGGCGAGGGCTGGGCTGCCCGCGGGTTTG
  GCTCTCTGCCCTGCATCCTCAACGCCTGCTCCGGAGATGCCAAGTCCACCTCCTGCATCA
  ACCTGTCAGTCACAGATGCTGGGGGCAGCGCCCAGCGCCCGCGGCCCCGCGTGCTGCTCA
  AGGCGCCTACCTTGGCTGAAATGGAAGAGATGAATACATCTGAGGAAGAAGAGTCTCCGT
  GTGGGGAGGTGACGCTGAAACGGGACCGCTCCTTCTCAGAGCATGACCTGGCCCAGCTCC
  GAAGTGAGGTGGCCTCTGGCCTGCAGTCGGCCACCCACCCCCCAGGAGGGACGGAGCCAC
  CACGGCCCCGAGCTGGCAGTGCGCATGTGTGGAGGCCCGGCTCCCGAGACCAGGGTACCT
  GTCCCGCGAGTGTCTCTGACCCCAGCCACCCCCTACTCAGCAACCGGGGCTCCTCGGGGG
  ACAGACCCGAGGCCTCCATGACCCCCGATGCCAAACACCTGTGGCTGGAGTTCAGCCACC
  CCGTGGAGAGCCTGGCGCTGACTGTGGAAGAGGTGATGGACGTGCGCCGTGTGCTGGTGA
  AGGCCGAGATGGAAAAGTTTTTGCAGAACAAGGAGCTCTTCAGCAGTCTGAAGAAGGGGA
  AGATTTGCTGCTGCTGCCGGGCCAAGTTCCCGCTGTTCTCGTGGCCGCCCAGCTGTCTCT
  TCTGCAAGAGAGCCGTCTGCACTTCCTGTAGCATAAAGATGAAGATGCCTTCTAAGAAAT
  TTGGACACATCCCTGTCTACACACTGGGCTTTGAGAGTCCTCAGAGGGTATCAGCTGCCA
  AAACCGCGCCAATCCAGAGAAGAGACATCTTTCAGTCTCTGCAAGGGCCACAGTGGCAGA
  GCGTGGAGGAGGCGTTCCCCCACATCTACTCCCACGGCTGTGTCCTGAAGGATGTCTGCA
  GTGAGTGCACCAGCTTTGTGGCAGACGTGGTGCGTTCCAGCCGCAAGAGCGTGGACGTCC
  TCAACACTACGCCACGACGCAGTCGCCAGACCCAATCCCTCTACATCCCTAACACCAGGA
  CTCTTGACTTCAAGTGACAGCCCCAGGTGGCCAGGCCTCCAGGAGGCACCAGGCAGGCCC
  TGTATCAGGCTAGGACGCTCTGAGCTGTGCATGTACATATATACATATATAGATACATTT
  ATAATATATACACACAGTCTATATATTTATATACACTGTTTCCTGGCCCCAGAGCTCATT
  TGGGTTCAGGCGCACTTCAAAACCCTCCCTGGGGGAGGCTGTTTCTTCTCAGGATTCCTT
  GCCAGGGAGGAAGGGGAGGGAACAGGGTGGGTTTTCTCACTGAAGAGAGAAAGCAGAAGG
  TTCTAGATCCTGGCACAGACTGCATCCCATGTTCCCATGCTCTTCTCCGTCCCCAGGAAT
  GCGAACGGCAGTTTCCCTTCCCCAGTGGACGTCTAGGTGGGGACAGGGTATCTTGGCTCC
  CAGCTGGACCAGAGTGCCCTGCTTGCCTCTGCTCTCCCTTTGTGGGGACTCAGGCAGCAG
  AGGCATCTGGGAAGTCTCTGAGTAGGCAGGGTCCTCCTGGGAGGCACCCCCACCTGTTTG
  AAAGGTCTGGCCAGGCGTGGTGGTTCAGGCCTGTAATTCCAGCACTTTGGGAGGCCGAGG
  AGGGAGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGATGAAATGTTG
  TCTCTACTGAAAATGCAAAAATTAGCCAGGTATAGTGGCAGGAACCTGTAATCCCAGCTA
  CAGGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGTGTAGGTTGCAGTGAGCCG
  AGATTGCACCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCTGTCTCGAAAAAAAAA
  AAGGTCCGTGCCAAGCTGCTCCCTGCCCTTGCCCTTTCCCTTTCCCTGGGGTCCAAACCA
  CATGTGTCCTGCCTCTCCTGGCCCTACCACATTCTGGTGCTGTCCTCACTCGCCCCTGGC
  CCAGAGGCTCCTGAAGATGCTGGGCGGTCCTGGCACAGGGAGGAGCAGCTCTGTAAATCT
  GTGCACATGGCCACTCTTGGCCTAATAAAGGAGGTCTCACAGTCAAAAAAAAAAAAAAAA
  AAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032451 unedited
  TTGGGCAGTTAGATATTTGTATACCATCATATAGGCGGCCGACGAATTCGCACGAGGTCC
  GGCGCGCGGGAGGCGATGACGGCCCCGCCATGGCCCGGGCGGGCAGCTGCGGCGGCGCCG
  CGGCGGGCGCAGGGCGGCCGGAGCCCTGGGAGCTGTCCCTGGAGGAGGTGCTGAAGGCCT
  ACGAGCAGCCGCTCAACGAGGAGCAGGCGTGGGCCGTGTGCTTCCAGGGCTGCCGCGGGC
  TGCGGGGCTCGCCGGGCCGGCGCCTGCGGGATACCGGGGACCTCCTGCTGCGCGGGGACG
  GCTCGGTCGGGGCGCGGGAGCCCGAGGCCGCGGAACCTGCAACCATGGTCGTGCCACTAG
  CCAGCTCGGAAGCCCAGACCGTGCAGTCCCTCGGCTTCGCCATCTACCGCGCGCTGGACT
  GGGGGCTGGACGAGAGCGAGGAGCGCGAACTCAGCCCTCAGCTGGAGCGGCTCATCGACC
  TCATGGCCAACAACGACAGCGAGGACAGCGGCTGCGGTGCCGCCGATGAGGGCTACGGNG
  GTCCCGAGGAGGAGGAGGAGGCCGAGGGCGTCCCCCGCAGCGTGCGCACCTTTGCCCAGG
  CCATGCGGCTGTGCGCGGCGCGGCTGACCGACCCCCGGGGCGCACAGGCGCATTACCAGG
  CCGTGTGCCGCGCGCTCTTCGTGGAGACGCTGGAGCTGCGGGCCTTCCTGGCCAGNGTCC
  GGGAGGCCAAGGAGATGCTGCAGAAACTTCGGGAGAACGAGCCGGATCTGGAGACGCCTC
  GGGCAGAGCTGGACAGCCCTGGTCACACAGACTGGGCCCGACTGTGGGTTCAGCTCATGC
  GGGAGCTCCGCCGCGGAGTGAAACTGAAAAAAGTGCAAGAACAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032451 unedited
  NNNCCGCGCATTGGANGATGGCACTTNCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACA
  GGATGCCACCCGGGTTCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAG
  TTTTTTTTTTTTTTTTTTTTGACTGTGAGACCTCCTTTATTAGGCCAAGAGTGGCCATGT
  GCACAGATTTACAGAGCTGCTCCTCCCTGTGCCAGGACCGCCCAGCATCTTCAGGAGCCT
  CTGGGCCAGGGGCGAGTGAGGACAGCACCAGAATGTGGTAGGGCCAGGAGAGGCAGGACA
  CATGTGGTTTGGACCCCAGGGAAAGGGAAAGGGCAAGGGCAGGGAGCAGCTTGGCACGGA
  CCTTTTTTTTTTTCGAGACAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGTGCA
  ATCTCGGCTCACTGCAACCTACACCTCCCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCC
  CCTGTAGCTGGGATTACAGGTTCCTGCCACTATACCTGGCTAATTTTTGCATTTTCAGTA
  GAGACAACATTTCATCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCCT
  CCCTCCTCGGCCTCCCAAAGTGCTGGAATTACAGGCCTGAACCACCACGCCTGGCCAGAC
  CTTTCAAACAGGTGGGGGTGCCTCCCAGGAGGACCCTGCCTACTCAGAGACTTCCCAGAT
  GCCTCTGCTGCCTGAGTCCCCACAAGGGAGAGCAGAAGCAAGCAGGGCACTCTGGTCCAG
  CTGGGAGCCAAGATACCCTGTCCCCACCTAGACGTCCACTGGGGAAAGGAAACTGCCGTT
  CGCATTCCTGGGGACGGAGAAAACATTGGAACATGGNATGCATTCTGT
  
• Restriction Sites: Please inquire
• ACCN: NM_032451
• Insert Size: 3300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032451.1, NP_115827.1
• RefSeq Size: 3249 bp
• RefSeq ORF: 2145 bp
• Locus ID: 84501
• UniProt ID: Q8WWL2
• Cytogenetics: 16q24.3
• Protein Pathways: Dorso-ventral axis formation
• Gene Summary: Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (By similarity). Required for asymmetric spindle positioning and asymmetric cell division during meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PubMed:26287480).[UniProtKB/Swiss-Prot Function]