RBM25 (NM_021239) Human Untagged Clone

CAT#: SC304929

RBM25 (untagged)-Human RNA binding motif protein 25 (RBM25)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RBM25
• Synonyms: fSAP94; NET52; RED120; RNPC7; S164; Snu71
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021239 edited
  GCGAAGGCAGTACCGTTTCCTCAGCGGCGGACTGCTGCAGTAAGAATGTCTTTTCCACCT
  CATTTGAATCGCCCTCCCATGGGAATCCCAGCACTCCCACCAGGGATCCCACCCCCGCAG
  TTTCCAGGATTTCCTCCACCTGTACCTCCAGGGACCCCAATGATTCCTGTACCAATGAGC
  ATTATGGCTCCTGCTCCAACTGTCTTAGTACCCACTGTGTCTATGGTTGGAAAGCATTTG
  GGCGCAAGAAAGGATCATCCAGGCTTAAAGGCTAAAGAAAATGATGAAAATTGTGGTCCT
  ACTACCACTGTTTTTGTTGGCAACATTTCCGAGAAAGCTTCAGACATGCTTATAAGACAA
  CTCTTAGCTAAATGTGGTTTGGTTTTGAGCTGGAAGAGAGTACAAGGTGCTTCCGGAAAG
  CTTCAAGCCTTCGGATTCTGTGAGTACAAGGAGCCAGAATCTACCCTCCGTGCACTCAGA
  TTATTACATGACCTGCAAATTGGAGAGAAAAAGCTACTCGTTAAAGTTGATGCAAAGACA
  AAGGCACAGCTGGATGAATGGAAAGCAAAGAAGAAAGCTTCTAATGGGAATGCAAGGCCA
  GAAACTGTCACTAATGACGATGAAGAAGCCTTGGATGAAGAAACAAAGAGGAGAGATCAG
  ATGATTAAAGGGGCTATTGAAGTTTTAATTCGTGAATACTCCAGTGAGCTAAATGCCCCC
  TCACAGGAATCTGATTCTCACCCCAGGAAGAAGAAGAAGGAAAAGAAGGAGGACATTTTC
  CGCAGATTTCCAGTGGCCCCACTGATCCCTTATCCACTCATCACTAAGGAGGATATAAAT
  GCTATAGAAATGGAAGAAGACAAAAGAGACCTGATATCTCGAGAGATCAGCAAATTCAGA
  GACACACATAAGAAACTGGAAGAAGAGAAAGGCAAAAAGGAAAAAGAAAGACAGGAAATT
  GAGAAAGAACGGAGAGAAAGAGAGAGGGAGCGTGAAAGGGAACGAGAAAGGCGAGAACGG
  GAACGAGAAAGGGAAAGAGAACGTGAACGAGAAAAGGAGAAAGAACGGGAGCGGGAACGA
  GAACGGGATAGGGACCGTGACCGGACAAAAGAGAGAGACCGAGATCGGGATCGAGAGAGA
  GATCGTGACCGGGATAGAGAAAGGAGCTCAGATCGTAATAAGGATCGCAGTCGATCAAGA
  GAAAAAAGCAGAGATCGTGAAAGGGAACGAGAGCGGGAAAGAGAGAGAGAGAGAGAACGA
  GAGCGAGAACGAGAACGGGAGCGAGAGAGAGAGCGAGAGAGGGAACGGGAGCGAGAAAGA
  GAAAAAGACAAAAAACGGGACCGAGAAGAAGATGAAGAAGATGCATACGAACGAAGAAAA
  CTTGAAAGAAAACTCCGAGAGAAAGAAGCTGCTTATCAAGAGCGCCTTAAGAATTGGGAA
  ATCAGAGAACGAAAGAAAACCCGGGAATATGAGAAAGAAGCTGAAAGAGAAGAAGAAAGA
  AGAAGAGAAATGGCCAAAGAAGCTAAACGACTAAAAGAATTCTTAGAAGACTATGATGAT
  GATAGAGATGACCCCAAATATTACAGAGGAAGTGCTCTTCAGAAAAGGTTGCGTGATAGA
  GAAAAGGAAATGGAAGCAGATGAACGAGATAGGAAGAGAGAGAAGGAGGAGCTTGAGGAA
  ATCAGGCAGCGCCTTCTGGCAGAAGGGCATCCAGATCCAGATGCAGAGCTCCAGAGGATG
  GAACAAGAGGCTGAGAGGCGCAGGCAGCCACAAATAAAGCAAGAGCCAGAATCAGAAGAG
  GAGGAAGAAGAAAAGCAAGAAAAAGAAGAAAAACGAGAAGAACCCATGGAAGAGGAAGAG
  GAGCCAGAGCAAAAGCCTTGTCTGAAACCTACTCTGAGGCCCATCAGCTCTGCTCCATCT
  GTTTCCTCTGCCAGTGGCAATGCAACACCTAACACTCCTGGGGATGAGTCTCCCTGTGGT
  ATTATTATTCCTCATGAAAACTCACCAGATCAACAGCAACCTGAGGAGCATAGGCCAAAA
  ATAGGACTAAGTCTTAAACTGGGTGCTTCCAATAGTCCTGGTCAGCCTAATTCTGTGAAG
  AGAAAGAAACTACCTGTAGATAGTGTCTTTAACAAATTTGAGGATGAAGACAGTGATGAC
  GTACCCCGAAAAAGGAAACTGGTTCCCTTGGATTATGGTGAAGATGATAAAAATGCAACC
  AAAGGCACTGTAAACACTGAAGAAAAGCGTAAACACATTAAGAGTCTCATTGAGAAAATC
  CCTACAGCCAAACCTGAGCTCTTCGCTTATCCCCTGGATTGGTCTATTGTGGATTCTATA
  CTGATGGAACGTCGAATTAGACCATGGATTAATAAGAAAATCATAGAATATATAGGTGAA
  GAAGAAGCTACATTAGTTGATTTTGTTTGTTCTAAGGTTATGGCTCATAGTTCACCCCAG
  AGCATTTTAGATGATGTTGCCATGGTACTTGATGAAGAAGCAGAAGTTTTTATAGTCAAA
  ATGTGGAGATTATTGATATATGAAACAGAAGCCAAGAAAATTGGTCTTGTGAAGTAAAAC
  TTTTTATATTTAGAGTTCCATTTCAGATTTCTTCTTTGCCACCCTTTTAAGGACTTTGAA
  TTTTTCTTTGTCTTTGAAGACATTGTGAGATCTGTAATTTTTTTTTTTTTTGTAGAAAAT
  GTGAATTTTTTGGTCCTCTAATTTGTTGTTGCCCTGTGTACTC
  
• Restriction Sites: Please inquire
• ACCN: NM_021239
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_021239.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021239.1, NP_067062.1
• RefSeq Size: 4189 bp
• RefSeq ORF: 2532 bp
• Locus ID: 58517
• UniProt ID: P49756
• Cytogenetics: 14q24.2
• Protein Pathways: Spliceosome
• Gene Summary: RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.[UniProtKB/Swiss-Prot Function]