DFNB31 (WHRN) (NM_015404) Human Untagged Clone

CAT#: SC304310

DFNB31 (untagged)-Human deafness, autosomal recessive 31 (DFNB31), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DFNB31
• Synonyms: CIP98; DFNB31; PDZD7B; USH2D; WI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304310 representing NM_015404.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAACGCGCCGCTGGACGGCCTGTCGGTGAGCTCGTCCTCCACCGGCTCGCTGGGCTCGGCGGCCGGG
  GCGGGCGGCGGCGGGGGCGCGGGGCTGCGGTTACTGTCTGCCAACGTGCGCCAGCTGCACCAAGCGCTG
  ACCGCGCTGCTGAGCGAGGCGGAGCGGGAGCAGTTCACCCACTGCCTGAACGCTTACCACGCGCGCCGC
  AACGTCTTCGACCTGGTGCGCACCCTGCGCGTGCTGCTGGACAGTCCGGTCAAGCGGCGCCTGCTGCCC
  ATGCTTCGTCTGGTCATCCCGCGCTCCGACCAGCTGCTCTTCGACCAATACACGGCCGAGGGCCTCTAC
  CTGCCCGCCACCACCCCCTACAGGCAGCCCGCCTGGGGCGGCCCCGACAGCGCGGGGCCAGGGGAGGTG
  CGCCTGGTGAGTTTGCGGCGTGCCAAGGCCCACGAGGGCTTGGGCTTCAGCATCCGTGGGGGCTCGGAG
  CACGGCGTGGGCATCTACGTGTCTCTGGTGGAACCAGGCTCTCTAGCTGAGAAGGAAGGACTGCGGGTC
  GGGGACCAGATTCTGCGCGTCAACGACAAATCCCTGGCCCGGGTGACCCACGCGGAGGCCGTCAAGGCT
  CTGAAGGGCTCCAAGAAGCTGGTGCTGTCTGTGTACTCAGCAGGGCGCATCCCTGGGGGCTACGTCACC
  AACCACATCTACACCTGGGTGGACCCGCAGGGCCGCAGCATCTCCCCACCCTCGGGCCTGCCCCAGCCC
  CACGGTGGTGCCCTGAGGCAGCAGGAGGGTGACCGGAGGAGCACCCTGCACCTCCTGCAAGGAGGGGAT
  GAGAAAAAGGTGAACCTGGTGCTGGGGGACGGCCGGTCCCTGGGCCTCACGATCCGTGGGGGAGCTGAG
  TACGGCCTTGGCATTTACATCACTGGCGTGGACCCAGGCTCTGAAGCAGAAGGCAGCGGGCTCAAGGTT
  GGGGACCAGATTCTAGAAGTGAATGGGCGGAGCTTTCTCAACATCCTACACGACGAGGCTGTCAGGCTG
  CTTAAGTCATCTCGGCACCTCATCCTGACAGTGAAGGACGTCGGGAGGCTGCCCCATGCCCGCACCACT
  GTGGACGAGACCAAGTGGATCGCCAGTTCCCGGATCAGGGAGACCATGGCGAACTCGGCAGGGTTTCTT
  GGCGATCTCACAACAGAAGGAATAAACAAGCCAGGATTTTACAAGGGCCCAGCCGGCTCCCAGGTGACC
  CTGAGCAGCCTGGGGAACCAGACACGAGTGCTGCTGGAGGAGCAGGCTCGGCACCTGCTGAACGAGCAG
  GAACACGCCACCATGGCCTACTACCTGGATGAGTACCGTGGTGGCAGCGTCTCTGTGGAGGCCCTCGTC
  ATGGCCCTGTTCAAGCTGCTCAACACCCACGCCAAGTTCTCACTCCTCTCTGAGGTGAGAGGCACCATT
  TCCCCGCAAGACCTAGAACGCTTCGACCACCTGGTGCTGAGGCGTGAGATTGAGTCCATGAAGGCGCGG
  CAGCCCCCAGGCCCCGGGGCTGGGGACACCTACTCCATGGTCTCCTACAGTGACACGGGTTCATCCACA
  GGCAGCCACGGCACCTCCACCACCGTCAGCTCGGCCAGGAACACTCTGGACCTGGAGGAAACTGGCGAG
  GCTGTCCAGGGCAATATCAACGCCCTCCCAGATGTGTCCGTGGATGATGTCAGATCCACCTCCCAGGGG
  CTGTCAAGCTTCAAGCCACTGCCTCGCCCACCACCTCTGGCCCAAGGCAACGACCTCCCACTAGGCCAG
  CCAAGGAAGCTGGGGAGAGAGGACCTCCAGCCACCTTCCTCCATGCCTTCCTGCTCGGGCACTGTCTTC
  TCGGCTCCACAGAACCGCAGCCCGCCAGCGGGCACCGCACCCACCCCAGGGACCTCCTCTGCACAGGAC
  TTGCCCTCTTCCCCCATCTATGCCTCCGTCTCCCCTGCCAACCCCAGCTCCAAGAGGCCGCTGGACGCC
  CATCTGGCCCTGGTCAACCAACACCCCATCGGCCCCTTCCCACGGGTCCAGTCACCCCCGCACCTGAAA
  AGCCCCTCTGCAGAGGCCACAGTGGCTGGGGGCTGCCTTCTGCCCCCATCACCCTCTGGCCACCCAGAC
  CAGACAGGCACAAACCAGCACTTTGTCATGGTGGAGGTCCACCGCCCCGACAGCGAGCCAGACGTCAAT
  GAAGTGAGGGCGCTGCCCCAGACGCGCACAGCCTCTACGCTCTCCCAGCTCTCGGACAGCGGGCAGACT
  CTAAGCGAGGACAGTGGTGTGGATGCTGGCGAGGCAGAGGCCAGCGCCCCAGGCCGAGGAAGGCAGTCG
  GTGTCCACCAAGAGCAGGAGTAGCAAGGAGCTGCCTCGGAACGAGAGGCCCACAGATGGGGCCAACAAA
  CCGCCTGGACTTCTGGAGCCCACGTCCACTCTGGTCCGTGTGAAGAAAAGTGCGGCCACCCTGGGCATC
  GCCATCGAGGGTGGCGCCAACACCCGCCAGCCCCTGCCTAGGATTGTCACTATTCAGAGAGGCGGCTCA
  GCTCACAACTGTGGGCAGCTCAAGGTGGGCCACGTGATTCTGGAAGTGAATGGGCTGACGCTTCGGGGC
  AAGGAGCACCGGGAGGCCGCCCGCATTATCGCCGAGGCCTTCAAGACTAAGGACCGTGACTACATTGAC
  TTTCTGGTCACTGAGTTCAATGTGATGCTCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_015404
• Insert Size: 2724 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015404.3
• RefSeq Size: 4079 bp
• RefSeq ORF: 2724 bp
• Locus ID: 25861
• UniProt ID: Q9P202
• Cytogenetics: 9q32
• MW: 96.6 kDa
• Gene Summary: This gene is thought to function in the organization and stabilization of sterocilia elongation and actin cystoskeletal assembly, based on studies of the related mouse gene. Mutations in this gene have been associated with autosomal recessive non-syndromic deafness and Usher Syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).