ARHGEF4 (NM_015320) Human Untagged Clone

CAT#: SC304289

ARHGEF4 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGEF4
• Synonyms: ASEF; ASEF1; GEF4; SMIM39; STM6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015320 edited
  GCACCCCCACTTGCCTCCACACCTTTGTCCTCCAGTTTAGTTTCTCCAGAACACAGGAGG
  AAAAGTGAACCGACCATCAAGTGCACAGCCACCCAGGAAGGCGGTAGGTACCTACCTTCA
  GGTATCTTTCCGGAAAAGTCCTGGCTGGCGTCCCCCGGCAGCCCTCGGGCCCAGCAGGCT
  GGAATCGCACACACCCTGCCTTCCAGCTCTGCCTGCTGCCTGGCATATGAAAACCCCGGG
  ACGCCCTGCAGACCCACGAGCCCCAAGCCCCTGAGTCCCAGGCCTAGTGCTCAGCGGATG
  GGTCTCCACTACCCCGGGAGGGGTAGCGCCATCTCCATGGTTTCTCTTGGAAGCTACAGC
  TACGTGGACAGCAGTTCAGGGGACCCTGAAAGACCCAAGATTCCCAAGGGCCAGACCAGT
  TTCCTGCTTTCTCTGCAGACGCTAAACCAAGATGAGCAGAAGGAAGAGAGCAGGGAAGGA
  GGCCAGGGTCCGCGCGGCTTGGGCACAGTGCCCTGGCTCAGGGACCTTCCTGGGAGTGAG
  AACCACATGCCCTGGGAAGAACCAGCAGGTGAGAAGCCCAGTTGCTCTCACAGTCAGAAG
  GCATTCCACATGGAGCCTGCCCAGAAGCCCTGCTTCACCACTGACATGGTGACATGGGCC
  CTCCTCTGCATCTCTGCAGAGACTGTGCGTGGGGAGGCTCCTTCACAGCCTAGGGGCATC
  CCTCACCGCTCGCCCGTCAGTGTGGATGACCTGTGGCTGGAGAAGACACAGAGAAAGAAG
  TTGCAGAAGCAGGCCCACATCGAAAGGAGGCTGCACATAGGGGCAGTGCACAAAGATGGA
  GTCAAGTGCTGGAGAAAGACGATCATTACCTCTCCAGAGTCTTTGAATCTCCCTAGAAGA
  AGCCATCCACTCTCCCAGAGTGCTCCAACGGGACTGAACCACATGGGCTGGCCAGAGCAC
  ACACCAGGCACTGCCATGCCTGATGGAGCTCTGGACACAGCTGTCTGCGCTGACGAAGTG
  GGGAGCGAGGAGGACCTGTATGATGACCTGCACAGCTCCAGCCACCACTACAGCCACCCT
  GGAGGGGGTGGGGAGCAGCTGGCTATCAATGAGCTCATCAGCGATGGCAGTGTGGTCTGC
  GCTGAAGCACTCTGGGACCATGTCACCATGGACGACCAGGAGCTGGGCTTCAAAGCTGGG
  GACGTCATCGAAGTGATGGATGCCACCAACAGAGAGTGGTGGTGGGGCCGGGTCGCCGAT
  GGCGAGGGCTGGTTTCCAGCCAGCTTCGTTCGGCTGAGGGTGAATCAGGACGAGCCCGCG
  GATGACGACGCCCCTCTGGCCGGGAACAGCGGAGCGGAGGACGGCGGGGCGGAGGCGCAG
  AGCAGCAAGGACCAGATGCGGACCAACGTCATCAACGAGATCCTCAGCACTGAGCGGGAC
  TACATCAAGCACCTGCGCGACATCTGCGAGGGCTACGTCCGGCAGTGCCGCAAGCGCGCA
  GACATGTTCAGCGAGGAGCAGCTGCGTACCATCTTCGGGAACATCGAGGACATCTACCGC
  TGCCAGAAGGCCTTCGTGAAGGCCCTGGAGCAGAGGTTCAACCGCGAGCGCCCACACCTG
  AGCGAGCTGGGTGCCTGCTTCCTGGAGCATCAAGCCGACTTCCAGATCTACTCGGAGTAC
  TGCAATAACCACCCCAACGCCTGCGTGGAGCTCTCCCGGCTCACCAAGCTCAGCAAGTAC
  GTGTACTTCTTCGAGGCCTGCCGGCTGCTGCAGAAGATGATTGACATCTCCCTGGATGGC
  TTCCTGCTGACTCCGGTGCAGAAGATCTGCAAGTACCCTCTGCAGCTGGCCGAGCTGCTC
  AAATACACGCACCCCCAGCACAGGGACTTCAAGGATGTTGAAGCCGCCTTGCATGCCATG
  AAGAACGTGGCCCAGCTCATCAACGAGCGGAAGCGGAGACTTGAGAACATCGACAAGATT
  GCTCAGTGGCAGAGCTCCATAGAGGACTGGGAGGGAGAAGATCTCTTGGTCAGGAGCTCA
  GAACTCATCTACTCGGGGGAGCTGACTCGAGTTACACAGCCTCAAGCCAAAAGCCAGCAG
  CGAATGTTCTTTCTCTTTGACCACCAGCTCATCTACTGTAAGAAGGACCTGCTCCGCCGC
  GACGTGTTGTACTACAAGGGCCGGCTGGACATGGACGGCCTGGAGGTGGTGGACCTGGAG
  GACGGGAAGGACAGAGACCTCCATGTGAGCATCAAGAACGCCTTCCGGCTGCACCGTGGC
  GCCACAGGGGACAGCCACCTGCTGTGCACCAGGAAGCCCGAGCAGAAGCAGCGCTGGCTC
  AAGGCCTTTGCCAGGGAGAGGGAGCAGGTGCAGCTGGACCAGGAGACAGGCTTCTCCATC
  ACTGAACTGCAGAGGAAGCAGGCCATGCTGAATGCCAGCAAGCAGCAGGTCACAGGGAAG
  CCCAAAGCTGTTGGCCGGCCCTGCTACCTGACGCGCCAGAAGCACCCAGCCCTGCCCAGC
  AACCGGCCCCAGCAGCAGGTCCTGGTGCTGGCGGAGCCCAGGCGCAAGCCATCTACCTTC
  TGGCACAGCATCAGCCGGCTGGCACCCTTCCGCAAGTGAACTGGTCCCTGCCTGACAGCA
  CCTGCTGGGCCTTCCTGCCAGTGGCCCCCAGTTTTTCTTCCCCGAGGCCCACTCGGCCTG
  GCCTTCCTCTGCCTGCAAGTGAGCAGGGATGGGCTGGGGAGTTGCTTGTGCCACCAAGAC
  GTGCCAGGTCTGTACTCCTGTTGTCTTTTTCCCTGCTCCTGGTGCCCTGAAGAGACCAGC
  AAGGGGGCAGACCCCGCACTCGCCACACCGCCGCTGCAGCTTGGGCCCCATCCGCCCTCT
  GGACCTGTGTAGGGCCTCACTGCTGGAGCGGGGAAACCGCAGCTCAGCCCAGGCCCAGCT
  GGGGAGAAGGCGCTACCTGCGTGGGACCCTCTTCTCTGGAAACCTAATCCTCCTTTCATT
  TCCTCTGGGCAGGACTCTCTGGCCTTCTGTGGCCTGCAATGCCAGGCCATGTGCCCCTCT
  GCCCTCTAGTTCTCCAAGTCCCCAGCCCGGCCAGTGGTGCCAGGCAGCTTGCCACTTGGG
  AGGGCAGAAGCCAGGAATTCCACACCCTTGTGTTGCGCCCGGAGCCCGCCCTTCGCCTCC
  CAGCCCCTCAAGACACCGCTGGCTGCTGGACACCCTCTTCACTTGTGTGTGTGTGTGTAG
  CGGAAAAGGACAAGACGGTGCAGTCGGCTGCATACTCCCAGTCGGGAGTGTGGTCAGTCT
  GCCTGCTGCTGTGCGGTAGCTCCAGAACCACCTCGTTCCTGGTTTTGTTTGGATTTTGGC
  ATCTTGTTTTTCTAACAACAAACAATGGAGAAAAAGAATTGATTCTTAGTGACACAGAAG
  ATTGCCTTACGCTCGTGAGCGTGAGAAGCCATAAGAGAGAGACCGAATTCTGTGGCTCAG
  CACACAGGACTGACCCACAGCCCAGGCAGCGGGTGTGTGGAGATGGCGCCCTGTCCTGCC
  AAGGGGCGCCAGGAGCAGAGCCAGGGCCTGGCGAGCTGGCGTGGAGCCCACAGGATTCAG
  CAGCATGGACAGTCACTCTTGCACTATTCCTTCTCCAAGCCAGAAACCACATTTAATTTC
  ATAAATAAATTTATGAAAAGTAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015320 unedited
  NGTCAGCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGGCACCCCCAC
  TTGCCTCCACACCTTTGTCCTCCAGTTTAGTTTCTCCAGACACAGGAGGAAAAGTGAACC
  GACCATCAAGTGCACAGCCACCCAGGAAGGCGGTAGGTACCTACCTTCAGGTATCTTTCC
  GGAAAAGTCCTGGCTGGCGTCCCCCGGCAGCCCTCGGGCCCAGCAGGCTGGAATCGCACA
  CACCCTGCCTTCCAGCTCTGCCTGCTGCCTGGCATATGAAAACCCCGGGACGCCCTGCAG
  ACCCACGAGCCCCAAGCCCCTGAGTCCCAGGCCTAGTGCTCAGCGGATGGGTCTCCACTA
  CCCCGGGAGGGGTAGCGCCATCTCCATGGTTTCTCTTGGAAGCTACAGCTACGTGGACAG
  CAGTTCAGGGGACCCTGAAAGACCCAAGATTCCCAAGGGCCAGACCAGTTTCCTGCTTTC
  TCTGCAGACGCTAAACCAAGATGAGCAGAAGGAAGAGAGCAGGGAAGGAGGCCAGGGTCC
  GCGCGGCTTGGGCACAGTGCCCTGGCTCAGGGACCTTCCTGGGAGTGAGAACCACATGCC
  CTGNGAAGAACCAGCANGTGAGAAGCCCAGTTGCTCTCACAGTCAGAAGGCATTCCACAT
  GGAGCCTGCCCAGAAGCCCTGCTTCACCACTGACATGGTGACATGGGCCCTCCTCTGCAT
  CTCTGCAAAGACTGTGCGTGGGGAGGGCTCTTCACAGCCTAGGGGCATCCCTCACCGGTC
  GCCCGTCAGTGTGGATGACCTGTGGCTGGGAAANACCCCGAAAAGAAGTTGCAAAGCCAG
  CCCCACATCGAAAGGAGGCTGCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_015320 unedited
  CCTGGNGATGGCACTTCCGGCCAGAAGCACTGGGGAGGGTCACAGGGTGCCCCCGGGATC
  TGTTCAGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTT
  TACTTTTCATAAATTTATTTATGAAATTAAATGTGGTTTCTGGCTTGGAGAAGGAATAGT
  GCAAGAGTGACTGTCCATGCTGCTGAATCCTGTGGGCTCCACGCCAGCTCGCCAGGCCCT
  GGCTCTGCTCCTGGCGCCCCTTGGCAGGACAGGGCGCCATCTCCACACACCCGCTGCCTG
  GGCTGTGGGTCAGTCCTGTGTGCTGAGCCACAGAATTCGGTCTCTCTCTTATGGCTTCTC
  ACGCTCACGAGCGTAAGGCAATCTTCTGTGTCACTAAGAATCAATTCTTTTTCTCCATTG
  TTTGTTGTTAGAAAAACAAGATGCCAAAATCCAAACAAAACCAGGAACGAGGTGGTTCTG
  GAGCTACCGCACAGCAGCAGGCAGACTGACCACACTCCCGACTGGGAGTATGCAGCCGAC
  TGCACCGTCTTGTCCTTTTCCGCTACACCACACACACAAGTGAAGAGGGTGTCCAGCAGC
  CAGCGGTGTCTTGAGGGGCTGGGAGGCCAAAGGCGGGCTCCGGGCCAACACAAGGGTGTG
  GAATTCCTGGCTTCTGCCCTCCCAAGTGGCAAGCTGCCTGGCACCACTGGCCGGGCTGGG
  GACTTGGAGAACTAGAGGGCANAGGGGCACATGGCCTGGCATTGCAGGCCACAGAAGGCC
  AGAGAGTCCTGCCCCGAGAAATGAAAGGAGGTTAAGTTTCCACGAAAGAGGTCCCACGCA
  G
  
• Restriction Sites: Please inquire
• ACCN: NM_015320
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substituion of 1 aa.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015320.2, NP_056135.2
• RefSeq Size: 3667 bp
• RefSeq ORF: 2073 bp
• Locus ID: 50649
• UniProt ID: Q9NR80
• Cytogenetics: 2q21.1
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013]
  Transcript Variant: This variant (1) encodes the longer isoform (a).