FAM53B (NM_014661) Human Untagged Clone

CAT#: SC304152

FAM53B (untagged)-Human family with sequence similarity 53, member B (FAM53B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FAM53B
• Synonyms: bA12J10.2; KIAA0140; smp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304152 representing NM_014661.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTGATGGTCCTAAGTGAAAGCCTCAGCACCCGGGGAGCTGACTCCATTGCATGTGGGACCTTCAGC
  CGTGAACTGCACACGCCAAAGAAGATGAGTCAAGGACCTACACTTTTCTCTTGTGGAATTATGGAAAAT
  GACAGATGGCGAGACCTGGACAGGAAATGCCCTCTTCAGATTGACCAACCGAGCACCAGCATCTGGGAA
  TGCCTGCCTGAAAAGGACAGCTCACTATGGCACCGGGAGGCAGTGACCGCCTGCGCTGTGACCAGTCTG
  ATCAAAGACCTCAGCATCAGCGACCACAACGGGAACCCCTCAGCACCCCCTAGCAAGCGCCAGTGCCGC
  TCACTGTCCTTCTCCGATGAGATGTCCAGTTGCCGGACATCATGGAGGCCCTTGGGCTCCAAAGTCTGG
  ACTCCCGTGGAAAAGAGACGCTGCTACAGCGGGGGCAGCGTCCAGCGCTATTCCAACGGCTTCAGCACC
  ATGCAGAGGAGTTCCAGCTTCAGCCTCCCTTCCCGGGCCAACGTGCTCTCCTCACCCTGCGACCAGGCA
  GGACTCCACCACCGATTTGGAGGGCAGCCCTGCCAAGGGGTGCCAGGCTCAGCCCCGTGTGGACAGGCA
  GGTGACACCTGGAGCCCTGACCTGCACCCCGTGGGAGGAGGCCGGCTGGACCTGCAGCGGTCCCTCTCT
  TGCTCACATGAGCAGTTTTCCTTTGTGGAATACTGTCCTCCCTCAGCCAACAGCACACCTGCCTCAACA
  CCAGAGCTGGCGAGACGCTCCAGCGGCCTTTCCCGCAGCCGCTCCCAGCCGTGTGTCCTTAACGACAAG
  AAGGTCGGTGTTAAAAGGCGGCGCCCTGAAGAAGTGCAAGAGCAGAGGCCTTCTCTAGACCTTGCCAAG
  ATGGCACAGAACTGTCAGACCTTCAGCAGCCTCAGCTGCCTGAGCGCAGGGACAGAGGACTGCGGTCCC
  CAGAGCCCCTTCGCCCGCCACGTCAGCAACACCAGGGCCTGGACCGCCCTGCTCTCAGCCTCCGGCCCA
  GGGGGCAGGACCCCCGCTGGGACCCCGGTCCCTGAGCCTCTTCCCCCTTCCTTCGACGACCACCTCGCC
  TGCCAGGAGGACCTGTCCTGTGAGGAGTCAGACAGCTGCGCCCTGGACGAGGATTGTGGCAGGAGAGCG
  GAGCCGGCTGCAGCCTGGCGGGACCGCGGGGCCCCTGGGAACAGCCTCTGCTCCCTGGACGGCGAGTTG
  GACATTGAGCAGATAGAGAAGAACTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014661
• Insert Size: 1269 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014661.3
• RefSeq Size: 5740 bp
• RefSeq ORF: 1269 bp
• Locus ID: 9679
• UniProt ID: Q14153
• Cytogenetics: 10q26.13
• Protein Families: Druggable Genome
• MW: 45.8 kDa
• Gene Summary: Acts as a regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) nuclear localization.[UniProtKB/Swiss-Prot Function]