PTPN22 (NM_012411) Human Untagged Clone

CAT#: SC303978

PTPN22 (untagged)-Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPN22
• Synonyms: LYP; LYP1; LYP2; PEP; PTPN8; PTPN22.5; PTPN22.6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303978 representing NM_012411.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACCAAAGAGAAATTCTGCAGAAGTTCCTGGATGAGGCCCAAAGCAAGAAAATTACTAAAGAGGAG
  TTTGCCAATGAATTTCTGAAGCTGAAAAGGCAATCTACCAAGTACAAGGCAGACAAAACCTATCCTACA
  ACTGTGGCTGAGAAGCCCAAGAATATCAAGAAAAACAGATATAAGGATATTTTGCCCTATGATTATAGC
  CGGGTAGAACTATCCCTGATAACCTCTGATGAGGATTCCAGCTACATCAATGCCAACTTCATTAAGGGA
  GTTTATGGACCCAAGGCTTATATTGCCACCCAGGGTCCTTTATCTACAACCCTCCTGGACTTCTGGAGG
  ATGATTTGGGAATATAGTGTCCTTATCATTGTTATGGCATGCATGGAGTATGAAATGGGAAAGAAAAAG
  TGTGAGCGCTACTGGGCTGAGCCAGGAGAGATGCAGCTGGAATTTGGCCCTTTCTCTGTATCCTGTGAA
  GCTGAAAAAAGGAAATCTGATTATATAATCAGGACTCTAAAAGTTAAGTTCAATAGTGAAACTCGAACT
  ATCTACCAGTTTCATTACAAGAATTGGCCAGACCATGATGTACCTTCATCTATAGACCCTATTCTTGAG
  CTCATCTGGGATGTACGTTGTTACCAAGAGGATGACAGTGTTCCCATATGCATTCACTGCAGTGCTGGC
  TGTGGAAGGACTGGTGTTATTTGTGCTATTGATTATACATGGATGTTGCTAAAAGATGGGAGTCAAGCA
  AAGCATTGTATTCCTGAGAAAAATCACACTCTCCAAGCAGACTCTTATTCTCCTAATTTACCAAAAAGT
  ACCACAAAAGCAGCAAAAATGATGAACCAACAAAGGACAAAAATGGAAATCAAAGAATCTTCTTCCTTT
  GACTTTAGGACTTCTGAAATAAGTGCAAAAGAAGAGCTAGTTTTGCACCCTGCTAAATCAAGCACTTCT
  TTTGACTTTCTGGAGCTAAATTACAGTTTTGACAAAAATGCTGACACAACCATGAAATGGCAGACAAAG
  GCATTTCCAATAGTTGGGGAGCCTCTTCAGAAGCATCAAAGTTTGGATTTGGGCTCTCTTTTGTTTGAG
  GGATGTTCTAATTCTAAACCTGTAAATGCAGCAGGAAGATATTTTAATTCAAAGGTGCCAATAACACGG
  ACCAAATCAACTCCTTTTGAATTGATACAGCAGAGAGAAACCAAGGAGGTGGACAGCAAGGAAAACTTT
  TCTTATTTGGAATCTCAACCACATGATTCTTGTTTTGTAGAGATGCAGGCTCAAAAAGTAATGCATGTT
  TCTTCAGCAGAACTGAATTATTCACTGCCATATGACTCTAAACACCAAATACGTAATGCCTCTAATGTA
  AAGCACCATGACTCTAGTGCTCTTGGTGTATATTCTTACATACCTTTAGTGGAAAATCCTTATTTTTCA
  TCATGGCCTCCAAGTGGTACCAGTTCTAAGATGTCTCTTGATTTACCTGAGAAGCAAGATGGAACTGTT
  TTTCCTTCTTCTCTGTTGCCAACATCCTCTACATCCCTCTTCTCTTATTACAATTCACATGATTCTTTA
  TCACTGAATTCTCCAACCAATATTTCCTCACTATTGAACCAGGAGTCAGCTGTACTAGCAACTGCTCCA
  AGGATAGATGATGAAATCCCCCCTCCACTTCCTGTACGGACACCTGAATCATTTATTGTGGTTGAGGAA
  GCTGGAGAATTCTCACCAAATGTTCCCAAATCCTTATCCTCAGCTGTGAAGGTAAAAATTGGAACATCA
  CTGGAATGGGGTGGAACATCTGAACCAAAGAAATTTGATGACTCTGTGATACTTAGACCAAGCAAGAGT
  GTAAAACTCCGAAGTCCTAAATCAGAACTACATCAAGATCGTTCTTCTCCCCCACCTCCTCTCCCAGAA
  AGAACTCTAGAGTCCTTCTTTCTTGCCGATGAAGATTGTATGCAGGCCCAATCTATAGAAACATATTCT
  ACTAGCTATCCTGACACCATGGAAAATTCAACATCTTCAAAACAGACACTGAAGACTCCTGGAAAAAGT
  TTCACAAGGAGTAAGAGTTTGAAAATTTTGCGAAACATGAAAAAGAGTATCTGTAATTCTTGCCCACCA
  AACAAGCCTGCAGAATCTGTTCAGTCAAATAACTCCAGCTCATTTCTGAATTTTGGTTTTGCAAACCGT
  TTTTCAAAACCCAAAGGACCAAGGAATCCACCACCAACTTGGAATATTTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_012411
• Insert Size: 2259 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012411.5
• RefSeq Size: 3513 bp
• RefSeq ORF: 2259 bp
• Locus ID: 26191
• UniProt ID: Q9Y2R2
• Cytogenetics: 1p13.2
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• MW: 85.1 kDa
• Gene Summary: This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (2) lacks two alternate in-frame exons in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note:.