MICB (NM_005931) Human Untagged Clone

CAT#: SC303706

MICB (untagged)-Human MHC class I polypeptide-related sequence B (MICB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MICB
• Synonyms: PERB11.2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005931 edited
  CCGGGATATCGTCGACCCACGCGTCCGAGCGGTCGCTGAGCGGGGCGCAGGTGACTAAAT
  TTCGACGGGGTCTTCTCACCGGTTTCATTCAGTTGGCCACTGCTGAGCAGCTGAGAAGGT
  GGCGACGTAGGGGCCATGGGGCTGGGCCGGGTCCTGCTGTTTCTGGCCGTCGCCTTCCCT
  TTTGCACCCCCGGCAGCCGCCGCTGAGCCCCACAGTCTTCGTTACAACCTCATGGTGCTG
  TCCCAGGATGGATCTGTGCAGTCAGGGTTTCTCGCTGAGGGACATCTGGATGGTCAGCCC
  TTCCTGCGCTATGACAGGCAGAAACGCAGGGCAAAGCCCCAGGGACAGTGGGCAGAAGAT
  GTCCTGGGAGCTGAGACCTGGGACACAGAGACCGAGGACTTGACAGAGAATGGGCAAGAC
  CTCAGGAGGACCCTGACTCATATCAAGGACCAGAAAGGAGGCTTGCATTCCCTCCAGGAG
  ATTAGGGTCTGTGAGATCCATGAAGACAGCAGCACCAGGGGCTCCCGGCATTTCTACTAC
  GATGGGGAGCTCTTCCTCTCCCAAAACCTGGAGACTCAAGAATCGACAGTGCCCCAGTCC
  TCCAGAGCTCAGACCTTGGCTATGAACGTCACAAATTTCTGGAAGGAAGATGCCATGAAG
  ACCAAGACACACTATCGCGCTATGCAGGCAGACTGCCTGCAGAAACTACAGCGATATCTG
  AAATCCGGGGTGGCCATCAGGAGAACAGTGCCCCCCATGGTGAATGTCACCTGCAGCGAG
  GTCTCAGAGGGCAACATCACCGTGACATGCAGGGCTTCCAGCTTCTATCCCCGGAATATC
  ACACTGACCTGGCGTCAGGATGGGGTATCTTTGAGCCACAACACCCAGCAGTGGGGGGAT
  GTCCTGCCTGATGGGAATGGAACCTACCAGACCTGGGTGGCCACCAGGATTCGCCAAGGA
  GAGGAGCAGAGGTTCACCTGCTACATGGAACACAGCGGGAATCACGGCACTCACCCTGTG
  CCCTCTGGGAAGGCGCTGGTGCTTCAGAGTCAACGGACAGACTTTCCATATGTTTCTGCT
  GCTATGCCATGTTTTGTTATTATTATTATTCTCTGTGTCCCTTGTTGCAAGAAGAAAACA
  TCAGCGGCAGAGGGTCCAGAGCTTGTGAGCCTGCAGGTCCTGGATCAACACCCAGTTGGG
  ACAGGAGACCACAGGGATGCAGCACAGCTGGGATTTCAGCCTCTGATGTCAGCTACTGGG
  TCCACTGGTTCCACTGAGGGCGCCTAGACTCTACAGCCAGGCGGCCAGGATTCAACTCCC
  TGCCTGGATCTCACCAGCACTTTCCCTCTGTTTCCTGACCTATGAAACAGAGAAAATAAC
  ATCACTTATTTATTGTTGTTGGATGCTGCAAAGTGTTAGTAGGTATGAGGTGTTTGCTGC
  TCTGCCACGTAGAGAGCCAGCAAAGGGATCATGACCAACTCAACATTCCATTGGAGGCTA
  TATGATCAAACAGCAAATTGTTTATCATGAATGCAGGATGTGGGCAAACTCACGACTGCT
  CCTGCCAACAGAAGGTTTGCTGAGGGCATTCACTCCATGGTGCTCATTGGAGTTATCTAC
  TGGGTCATCTAGAGCCTATTGTTTGAGGAATGCAGTCTTACAAGCCTACTCTGGACCCAG
  CAGTTGACTCCTTCTTCCACCCCTCTTCTTGCTATCTCCTATACCAATAAATACGAAGGG
  CTGTGGAAGATCAGAGCCCTTGTTCACGAGAAGCAAGAAGCCCCCTGACCCCTTGTTCCA
  AATATACTCTTTTGTCTTTCTCTTTATTCCCACGTTTGCCCTTTGTTCAGTCCAATACAG
  GGTTGTGGGGCCCTTAACAGTGCCATATTAATTGGTATCATTATTTCTGTTGTTTTTGTT
  TTTGTTTTTGTTTTTGTTTTTGAGACAGAGTCTCGCTCTGTCACCCAGGCTGCAGTTCAC
  TGGTGTGATCTCAGCTCACTGCAACCTCTGCCTCCCAGGTTCAAGCACTTCTCGTACCTC
  AGACTCCCGAATAGCTGGGATTACAGACAGGCACCACCACACCCAGCTAATTTTTGTATT
  TTTTGTAGAGACGGGGTTTCGCCAAGTTGACCAGCCCAGTTTCAAACTCCTGACCTCAGG
  TGATCTGCCTGCCTTGGCATCCCAAAGTGCTGGGATTACAAGAATGAGCCACCGTGCCTG
  GCCTATTTTATTATATTGTAATATATTTTATTATATTAGCCACCATGCCTGTCCTATTTT
  CTTATGTTTTAATATATTTTAATATATTACATGTGCAGTAATTAGATTATCATGGGTGAA
  CTTTATGAGTGAGTATCTTGGTGATGACTCCTCCTGACCAGCCCAGGACCAGCTTTCTTG
  TCACCTTGAGGTCCCCTCGCCCCGTCACACCGTTATGCATTACTCTGTGTCTACTATTAT
  GTGTGCATAATTTATACCGTAAATGTTTACTCTTTAAATAGAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005931 unedited
  TAGCTTTTGTATACGACTCCTATAGGGCGGCCGCTGAATTCAGATCTGGTACCGGTCCGG
  AATTCCCGGGATATCGTCGACCCACGCGTCCGAGCGGTCGCTGAGCGGGGCGCAGGTGAC
  TAAATTTCGACGGGGTCTTCTCACCGGTTTCATTCAGTTGGCCACTGCTGAGCAGCTGAG
  AAGGTGGCGACGTAGGGGCCATGGGGCTGGGCCGGGTCCTGCTGTTTCTGGCCGTCGCCT
  TCCCTTTTGCACCCCCGGCAGCCGCCGCTGAGCCCCACAGTCTTCGTTACAACCTCATGG
  TGCTGTCCCAGGATGGATCTGTGCAGTCAGGGTTTCTCGCTGAGGGACATCTGGATGGTC
  AGCCCTTCCTGCGCTATGACAGGCAGAAACGCAGGGCAAAGCCCCAGGGACAGTGGGCAG
  AAGATGTCCTGGGAGCTGAGACCTGGGACACAGAGACCGAGGACTTGACAGAGAATGGGC
  AAGACCTCAGGAGGACCCTGACTCATATCAAGGACCAGAAAGGAGGCTTGCATTCCCTCC
  AGGAGATTAGGGTCTGTGAGATCCATGAAGACAGCAGCACCAGGGGCTCCCGGCATTTCT
  ACTACGATGGGGAGCTCTTCCTCTCCCAAAACCTGGAGACTCAAGAATCGACAGTGCCCC
  AGTCCTCCAGAGCTCAGACCTTGGCTATGAACGTCACAAATTTCTGGAAGGAAGATGCCA
  TGAAGACCAAGACACACTATCGCGCTATGCAGGCAGACTGCCTGCAGAAACTACAGCGAT
  ATCTGAAATCCCGGGGTGGCCATCAGAGACAGTGCCCCCCATGGTGAATGTCACCTGCAG
  CGAGGTCTCAGAGGGCAACATCACCGTGACATGCAGGGCTTCCAGCTTCTATCCCCGGAA
  TATCCACACTGACCTG
  
• Restriction Sites: Please inquire
• ACCN: NM_005931
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005931.2, NP_005922.1
• RefSeq Size: 2385 bp
• RefSeq ORF: 1152 bp
• Locus ID: 4277
• UniProt ID: Q29980
• Cytogenetics: 6p21.33
• Domains: MHC_I, ig, IGc1
• Protein Families: Druggable Genome
• Protein Pathways: Natural killer cell mediated cytotoxicity
• Gene Summary: This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).