Exosome Component 9 (EXOSC9) (NM_001034194) Human Untagged Clone

CAT#: SC302795

EXOSC9 (untagged)-Human exosome component 9 (EXOSC9), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Exosome Component 9
• Synonyms: p5; p6; PCH1D; PM/Scl-75; PMSCL1; RRP45; Rrp45p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC302795 representing NM_001034194.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGGAAACGCCACTCTCAAACTGCGAACGCCGCTTCCTACTCCGTGCCATCGAAGAGAAGAAGCGG
  CTGGATGGCAGACAAACCTATGATTATAGGAACATCAGGATCTCATTTGGAACAGATTACGGATGCTGC
  ATTGTGGAACTTGGAAAAACAAGAGTTCTTGGACAGGTTTCCTGTGAACTTGTGTCTCCAAAACTCAAT
  CGGGCAACAGAAGGTATTCTTTTTTTTAACCTTGAACTCTCTCAGATGGCCGCTCCAGCTTTCGAACCT
  GGCAGGCAGTCAGATCTCTTGGTGAAGTTGAATCGACTCATGGAAAGATGTCTAAGAAATTCGAAGTGT
  ATAGACACTGAGTCTCTCTGTGTTGTTGCTGGTGAAAAGGTTTGGCAAATACGTGTAGACCTACATTTA
  TTAAATCATGATGGAAATATTATTGATGCTGCCAGCATTGCTGCAATCGTGGCCTTATGTCATTTCCGA
  AGACCTGATGTCTCTGTCCAAGGAGATGAAGTAACACTGTATACACCTGAAGAGCGTGATCCTGTACCA
  TTAAGTATCCACCACATGCCCATTTGTGTCAGTTTTGCCTTTTTCCAGCAAGGAACATATTTATTGGTG
  GATCCCAATGAACGAGAAGAACGTGTGATGGATGGCTTGCTGGTGATTGCCATGAACAAACATCGAGAG
  ATTTGTACTATCCAGTCCAGTGGTGGGATAATGCTACTAAAAGATCAAGTTCTGAGATGCAGTAAAATC
  GCTGGTGTGAAAGTAGCAGAAATTACAGAGCTAATATTGAAAGCTTTGGAGAATGACCAAAAAGTAAGG
  AAAGAAGGTGGAAAGTTTGGTTTTGCAGAGTCTATAGCAAATCAAAGGATCACAGCATTTAAAATGGAA
  AAGGCCCCTATTGATACCTCGGATGTAGAAGAAAAAGCAGAAGAAATCATTGCTGAAGCAGAACCTCCT
  TCAGAAGTTGTTTCTACACCTGTGCTATGGACTCCTGGAACTGCCCAAATTGGAGAGGGAGTAGAAAAC
  TCCTGGGGTGATCTTGAAGACTCTGAGAAGGAAGATGATGAAGGCGGTGGTGATCAAGCTATCATTCTT
  GATGGTATAAAAATGGACACTGGAGTAGAAGTCTCTGATATTGGAAGCCAAGAGCTGGGGTTTCACCAT
  GTTGGCCAGACTGGACTCGAGTTCCTGACCTCAGATGCTCCCATAATACTCTCAGATAGTGAAGAAGAA
  GAAATGATCATTTTGGAACCAGACAAGAATCCAAAGAAAATAAGAACACAGACCACCAGTGCAAAACAA
  GAAAAAGCACCAAGTAAAAAGCCAGTGAAAAGAAGAAAAAAGAAGAGAGCTGCCAATTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001034194
• Insert Size: 1371 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034194.1
• RefSeq Size: 1644 bp
• RefSeq ORF: 1371 bp
• Locus ID: 5393
• UniProt ID: Q06265
• Cytogenetics: 4q27
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation
• MW: 50.8 kDa
• Gene Summary: This gene encodes a component of the human exosome, a exoribonuclease complex which processes and degrades RNA in the nucleus and cytoplasm. This component may play a role in mRNA degradation and the polymyositis/scleroderma autoantigen complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).