AMPD3 (NM_001025390) Human Untagged Clone

CAT#: SC302401

AMPD3 (untagged)-Human adenosine monophosphate deaminase 3 (AMPD3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMPD3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC302401 representing NM_001025390.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCCAGGCTCAGCTGAGATGCCGCGGCAGTTTCCCAAGCTGAACATCTCTGAAGTGGATGAGCAA
  GTCCGGCTCCTGGCGGAGAAGGTGTTTGCTAAAGTGCTCCGAGAAGAGGACAGCAAAGATGCCCTGTCC
  CTGTTCACTGTCCCAGAGGACTGCCCCATCGGGCAAAAGGAAGCCAAGGAGAGGGAGCTGCAGAAGGAG
  CTGGCAGAGCAGAAGTCTGTGGAGACCGCAAAAAGAAAGAAAAGTTTCAAGATGATTCGGTCCCAGTCC
  CTGTCTCTGCAAATGCCGCCACAGCAAGATTGGAAGGGCCCCCCGGCAGCCAGTCCGGCCATGTCTCCC
  ACAACCCCTGTGGTCACTGGAGCCACTTCCCTGCCCACGCCAGCACCCTATGCCATGCCTGAGTTCCAG
  CGGGTCACCATCAGCGGAGATTACTGTGCCGGGATCACTTTGGAGGACTATGAGCAGGCAGCCAAGAGT
  CTGGCCAAGGCCCTAATGATCCGGGAGAAGTATGCGCGGCTCGCCTACCACCGCTTCCCGCGGATCACA
  TCCCAGTACCTGGGTCATCCGCGGGCGGATACTGCACCTCCGGAAGAGGGCCTTCCAGACTTCCACCCT
  CCTCCACTGCCCCAGGAAGACCCCTACTGCCTGGATGATGCACCCCCCAACCTGGATTACTTGGTCCAC
  ATGCAGGGGGGCATCCTCTTTGTGTATGATAACAAGAAGATGCTGGAGCACCAGGAGCCGCACAGCCTA
  CCCTACCCCGACCTGGAGACCTACACGGTGGACATGAGCCACATCCTGGCTCTCATCACCGATGGCCCC
  ACGAAAACCTATTGTCACCGGCGACTGAACTTTCTGGAATCCAAGTTCAGCCTTCATGAGATGTTAAAC
  GAAATGTCCGAGTTCAAAGAGTTGAAGAGTAACCCCCACCGGGACTTCTATAACGTGAGAAAGGTGGAC
  ACACACATCCATGCGGCCGCCTGCATGAACCAAAAGCATCTGCTGCGCTTCATCAAGCACACATACCAG
  ACGGAGCCTGACAGGACTGTGGCAGAGAAGCGGGGCCGGAAGATCACCCTGCGGCAGGTGTTTGACGGC
  CTGCACATGGACCCCTACGACCTCACTGTGGACTCACTGGATGTCCACGCGGGCCGGCAGACATTCCAC
  CGCTTTGACAAGTTCAACTCCAAATACAACCCTGTGGGGGCCAGTGAGCTGCGTGACCTGTATTTGAAA
  ACTGAAAACTATCTGGGAGGAGAGTACTTTGCTCGGATGGTCAAGGAGGTTGCCCGGGAGCTGGAGGAG
  AGCAAGTACCAGTACTCAGAGCCACGGCTCTCCATCTACGGCCGCAGTCCTGAGGAGTGGCCCAACCTG
  GCCTACTGGTTCATCCAGCACAAGGTCTACTCTCCCAACATGCGCTGGATCATCCAGGTGCCCCGGATT
  TATGACATATTTAGGTCAAAGAAGCTGCTGCCAAACTTTGGGAAGATGCTGGAGAACATCTTCCTGCCC
  CTTTTCAAGGCCACTATCAACCCCCAAGATCATCGAGAGCTTCACCTCTTCCTTAAATATGTGACGGGG
  TTTGACAGCGTGGATGATGAGTCCAAGCACAGCGACCACATGTTTTCCGACAAGAGCCCAAACCCGGAC
  GTCTGGACCAGTGAGCAGAACCCACCCTACAGCTACTACCTGTACTACATGTATGCCAACATCATGGTG
  CTCAACAACCTCCGCAGGGAGCGCGGCCTGAGCACGTTCCTGTTCCGGCCGCACTGTGGGGAAGCCGGC
  TCCATCACCCACCTGGTGTCTGCCTTCCTCACTGCTGACAACATTTCCCACGGGCTGCTCCTCAAGAAG
  AGTCCGGTATTGCAGTATCTCTACTACCTTGCTCAGATCCCCATTGCCATGTCTCCTCTTAGCAACAAC
  AGTTTGTTCCTCGAATATTCCAAGAACCCTCTGAGGGAATTCCTACACAAGGGACTGCATGTTTCTCTT
  TCCACCGATGACCCCATGCAGTTCCACTACACGAAGGAAGCACTTATGGAAGAATATGCCATTGCAGCT
  CAAGTGTGGAAGCTGAGCACCTGCGACCTGTGTGAGATCGCCAGGAACAGCGTGCTGCAGAGCGGCCTC
  TCGCATCAGGAAAAGCAAAAGTTTCTGGGACAAAATTATTATAAAGAAGGACCTGAAGGAAATGATATT
  CGAAAGACAAATGTGGCTCAGATCCGGATGGCATTCCGATATGAGACCTTATGCAATGAGCTCAGCTTC
  CTGTCTGATGCTATGAAATCAGAAGAGATCACCGCCTTGACCAACTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025390
• Insert Size: 2325 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025390.1
• RefSeq Size: 4473 bp
• RefSeq ORF: 2325 bp
• Locus ID: 272
• UniProt ID: Q01432
• Cytogenetics: 11p15.4
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• MW: 89.5 kDa
• Gene Summary: This gene encodes a member of the AMP deaminase gene family. The encoded protein is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. This gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. Mutations in this gene lead to the clinically asymptomatic, autosomal recessive condition erythrocyte AMP deaminase deficiency. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) contains an alternate, in-frame exon for its 5' UTR and 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 1C, which has a shorter, distinct N-terminus, compared to isoform 1A.