CHI3L2 (NM_001025197) Human Untagged Clone

CAT#: SC302362

CHI3L2 (untagged)-Human chitinase 3-like 2 (CHI3L2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHI3L2
• Synonyms: CHIL2; YKL-39; YKL39
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC302362 sequence for NM_001025197 edited (data generated by NextGen Sequencing)
  ATGGGAGCAACCACCATGGACCAGAAGTCTCTCTGGGCAGGATCTGCCTACAAACTGGTT
  TGCTACTTTACCAACTGGTCCCAGGACCGGCAGGAACCAGGAAAATTCACCCCTGAGAAT
  ATTGACCCCTTCCTATGCTCTCATCTCATCTATTCATTCGCCAGCATCGAAAACAACAAG
  GTTATCATCAAGGACAAGAGTGAAGTGATGCTCTACCAGACCATCAACAGTCTCAAAACC
  AAGAATCCCAAACTGAAAATTCTCTTGTCCATTGGAGGGTACCTGTTTGGTTCCAAAGGG
  TTCCACCCTATGGTGGATTCTTCTACATCACGCTTGGAATTCATTAACTCCATAATCCTG
  TTTCTGAGGAACCATAACTTTGATGGACTGGATGTAAGCTGGATCTACCCAGATCAGAAA
  GAAAACACTCATTTCACTGTGCTGATTCATGAGTTAGCAGAAGCCTTTCAGAAGGACTTC
  ACAAAATCCACCAAGGAAAGGCTTCTCTTGACTGTGGGCGTATCTGCAGGGAGGCAAATG
  ATTGATAACAGCTATCAAGTTGAGAAACTGGCAAAAGATCTGGATTTCATCAACCTCCTG
  TCCTTTGACTTCCATGGGTCTTGGGAAAAGCCCCTTATCACTGGCCACAACAGCCCTCTG
  AGCAAGGGGTGGCAGGACAGAGGGCCAAGCTCCTACTACAATGTGGAATATGCTGTGGGG
  TACTGGATACATAAGGGAATGCCATCAGAGAAGGTGGTCATGGGCATCCCCACATATGGG
  CACTCCTTCACACTGGCCTCTGCAGAAACCACCGTGGGGGCCCCTGCCTCTGGCCCTGGA
  GCTGCTGGACCCATCACAGAGTCTTCAGGCTTCCTGGCCTATTATGAGATCTGCCAGTTC
  CTGAAAGGAGCCAAGATCACGCGGCTCCAGGATCAGCAGGTTCCCTACGCAGTCAAGGGG
  AACCAGTGGGTGGGCTATGATGATGTGAAGAGTATGGAGACCAAGGTTCAGTTCTTAAAG
  AATTTAAACCTGGGAGGAGCCATGATCTGGTCTATTGACATGGATGACTTCACTGGCAAA
  TCCTGCAACCAGGGCCCTTACCCTCTTGTCCAAGCAGTCAAGAGAAGCCTTGGCTCCCTG
  TGA
  
  Clone variation with respect to NM_001025197.1
  515 c=>t
• Restriction Sites: Please inquire
• ACCN: NM_001025197
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone is found to be a perfect match to NM_001025197.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025197.1, NP_001020368.1
• RefSeq Size: 1456 bp
• RefSeq ORF: 1143 bp
• Locus ID: 1117
• UniProt ID: Q15782
• Cytogenetics: 1p13.2
• Gene Summary: The protein encoded by this gene is similar to bacterial chitinases but lacks chitinase activity. The encoded protein is secreted and is involved in cartilage biogenesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.