APLP1 (NM_001024807) Human Untagged Clone

CAT#: SC302299

APLP1 (untagged)-Human amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APLP1
• Synonyms: APLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC302299 representing NM_001024807.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGCCCGCCAGCCCCGCTGCTCGCGGTCTAAGTCGCCGCCCGGGCCAGCCGCCGCTGCCGCTGCTG
  CTGCCACTATTGCTGCTGCTTCTGCGCGCGCAGCCCGCCATCGGGAGCCTGGCCGGTGGGAGCCCCGGC
  GCGGCCGAGGCCCCGGGGTCGGCCCAGGTGGCTGGACTATGCGGGCGCCTAACCCTTCACCGGGACCTG
  CGCACCGGCCGCTGGGAACCAGACCCACAGCGCTCTCGACGCTGTCTCCGGGACCCGCAGCGCGTGCTG
  GAGTACTGCAGACAGATGTACCCGGAGCTGCAGATTGCACGTGTGGAGCAGGCTACGCAGGCCATCCCC
  ATGGAGCGCTGGTGCGGGGGTTCCCGGAGCGGCAGCTGCGCCCACCCCCACCACCAGGTTGTGCCCTTC
  CGCTGCCTGCCTGGTGAATTTGTGAGTGAGGCCCTGCTGGTGCCTGAAGGCTGCCGGTTCTTGCACCAG
  GAGCGCATGGACCAATGTGAGAGTTCAACCCGGAGGCATCAGGAGGCACAGGAGGCCTGCAGCTCCCAG
  GGCCTCATCCTGCACGGCTCGGGCATGCTCTTACCCTGTGGCTCGGATCGGTTCCGTGGTGTGGAGTAT
  GTGTGCTGTCCCCCTCCAGGGACCCCCGACCCATCTGGGACAGCAGTTGGTGACCCCTCCACCCGGTCC
  TGGCCCCCGGGGAGCAGAGTAGAGGGGGCTGAGGACGAGGAAGAGGAGGAATCCTTCCCACAGCCAGTA
  GATGATTACTTCGTGGAGCCTCCGCAGGCTGAAGAGGAAGAGGAAACGGTCCCACCCCCAAGCTCCCAT
  ACACTTGCAGTGGTCGGCAAAGTCACTCCCACCCCGAGGCCCACAGACGGTGTGGATATTTACTTTGGC
  ATGCCTGGGGAAATCAGTGAGCACGAGGGGTTCCTGAGGGCCAAGATGGACCTGGAGGAGCGTAGGATG
  CGCCAGATTAATGAGGTGATGCGTGAATGGGCCATGGCAGACAACCAGTCCAAGAACCTGCCTAAAGCC
  GACAGACAGGCCCTGAATGAGCACTTCCAGTCCATTCTGCAGACTCTGGAGGAGCAGGTGTCTGGTGAG
  CGACAGCGCCTGGTGGAAACCCACGCCACCCGCGTCATCGCCCTTATCAACGACCAGCGCCGGGCTGCC
  TTGGAGGGCTTCCTGGCAGCCCTGCAGGCAGATCCGCCTCAGGCGGAGCGTGTCCTGTTGGCCCTGCGG
  CGCTACCTGCGTGCGGAGCAGAAGGAACAGAGGCACACGCTGCGCCACTACCAGCATGTGGCCGCCGTG
  GATCCCGAGAAGGCACAGCAGATGCGCTTCCAGGTGCATACCCACCTTCAAGTGATTGAGGAGAGGGTG
  AATCAGAGCCTGGGCCTGCTTGACCAGAACCCCCACCTGGCTCAGGAGCTGCGGCCCCAAATCCAGGAA
  CTCCTCCACTCTGAACACCTGGGTCCCAGTGAATTGGAAGCCCCTGCCCCTGGGGGCAGCAGCGAGGAC
  AAGGGTGGGCTGCAGCCTCCAGATTCCAAGGATGCAGACACCCCCATGACCCTTCCAAAAGGGTCCACA
  GAACAAGATGCTGCATCCCCTGAGAAAGAGAAGATGAACCCGCTGGAACAGTATGAGCGAAAGGTGAAT
  GCGTCTGTTCCAAGGGGTTTCCCTTTCCACTCATCGGAGATTCAGAGGGATGAGCTGGCACCAGCTGGG
  ACAGGGGTGTCCCGTGAGGCTGTGTCGGGTCTGCTGATCATGGGAGCGGGCGGAGGCTCCCTCATCGTC
  CTCTCCATGCTGCTCCTGCGCAGGAAGAAGCCCTACGGGGCTATCAGCCATGGCGTGGTGGAGGTGGAC
  CCCATGCTGACCCTGGAGGAGCAGCAGCTCCGCGAACTGCAGCGGCACGGCTATGAGAACCCCACTTAC
  CGCTTCCTGGAGGAACGACCCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024807
• Insert Size: 1956 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024807.2
• RefSeq Size: 2517 bp
• RefSeq ORF: 1956 bp
• Locus ID: 333
• UniProt ID: P51693
• Cytogenetics: 19q13.12
• Protein Families: Druggable Genome, Transmembrane
• MW: 72.2 kDa
• Gene Summary: This gene encodes a member of the highly conserved amyloid precursor protein gene family. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).